Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/82950 |
Resumo: | The development of optical biosensors based on structural colors generated by short-range ordered colloidal particles is attracting growing interest due to their non-iridescent and non-fading features. In this study, a biomimetic approach using biopolymers for the various steps of sensor construction is presented. Bacterial nanocellulose (BNC) has many foreseen applications in biomedical engineering because of its biocompatibility, good mechanical strength, and large modifiable surface area. Herein, a novel approach is taken by using functionalized BNC as a substrate to build a molecularly imprinted photonic sensing layer. BNC was modified with polydopamine (PDA), which improved the adhesion and mechanical properties of the BNC substrate while providing simultaneously a black background for color saturation. A molecularly imprinted polymer (MIP) also made of PDA was used to create the recognition sites for the biomarker lysozyme. A monodisperse colloidal suspension of silica particles was first synthesized and used as core of the MIP shell, and then the photonic structure was assembled on the PDA-BNC membrane. The biosensor showed a detection limit of about 0.8nmolL1 of lysozyme in spiked human serum and demonstrated to be selective against cystatin C. These properties, combined with biocompatible, eco-friendly, and low-cost materials, offer a sustainable sensing platform with great potential for healthcare applications. |
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Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensingBacterial nanocellulosePolydopamineMolecular imprinting technologyPhotonic polymersLysozymeThe development of optical biosensors based on structural colors generated by short-range ordered colloidal particles is attracting growing interest due to their non-iridescent and non-fading features. In this study, a biomimetic approach using biopolymers for the various steps of sensor construction is presented. Bacterial nanocellulose (BNC) has many foreseen applications in biomedical engineering because of its biocompatibility, good mechanical strength, and large modifiable surface area. Herein, a novel approach is taken by using functionalized BNC as a substrate to build a molecularly imprinted photonic sensing layer. BNC was modified with polydopamine (PDA), which improved the adhesion and mechanical properties of the BNC substrate while providing simultaneously a black background for color saturation. A molecularly imprinted polymer (MIP) also made of PDA was used to create the recognition sites for the biomarker lysozyme. A monodisperse colloidal suspension of silica particles was first synthesized and used as core of the MIP shell, and then the photonic structure was assembled on the PDA-BNC membrane. The biosensor showed a detection limit of about 0.8nmolL1 of lysozyme in spiked human serum and demonstrated to be selective against cystatin C. These properties, combined with biocompatible, eco-friendly, and low-cost materials, offer a sustainable sensing platform with great potential for healthcare applications.EC -European Commission(FET-Open/H2020/GA829040)info:eu-repo/semantics/publishedVersionElsevier B.V.Universidade do MinhoSuleimenova, AkmaralFrasco, Manuela FariaSilva, Francisco Almeida Garrett SoaresGama, F. M.Fortunato, ElviraSales, M. Goreti F.2023-052023-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/82950engSuleimenova, Akmaral; Frasco, Manuela; Soares da Silva, F. A. G.; Gama, F. M.; Fortunato, Elvira; Sales, M. G. F., Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing. Biosensors and Bioelectronics: X, 13(100310), 20232590-137010.1016/j.biosx.2023.100310https://www.sciencedirect.com/journal/biosensors-and-bioelectronics-xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:22Zoai:repositorium.sdum.uminho.pt:1822/82950Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:21.300332Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
title |
Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
spellingShingle |
Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing Suleimenova, Akmaral Bacterial nanocellulose Polydopamine Molecular imprinting technology Photonic polymers Lysozyme |
title_short |
Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
title_full |
Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
title_fullStr |
Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
title_full_unstemmed |
Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
title_sort |
Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
author |
Suleimenova, Akmaral |
author_facet |
Suleimenova, Akmaral Frasco, Manuela Faria Silva, Francisco Almeida Garrett Soares Gama, F. M. Fortunato, Elvira Sales, M. Goreti F. |
author_role |
author |
author2 |
Frasco, Manuela Faria Silva, Francisco Almeida Garrett Soares Gama, F. M. Fortunato, Elvira Sales, M. Goreti F. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Suleimenova, Akmaral Frasco, Manuela Faria Silva, Francisco Almeida Garrett Soares Gama, F. M. Fortunato, Elvira Sales, M. Goreti F. |
dc.subject.por.fl_str_mv |
Bacterial nanocellulose Polydopamine Molecular imprinting technology Photonic polymers Lysozyme |
topic |
Bacterial nanocellulose Polydopamine Molecular imprinting technology Photonic polymers Lysozyme |
description |
The development of optical biosensors based on structural colors generated by short-range ordered colloidal particles is attracting growing interest due to their non-iridescent and non-fading features. In this study, a biomimetic approach using biopolymers for the various steps of sensor construction is presented. Bacterial nanocellulose (BNC) has many foreseen applications in biomedical engineering because of its biocompatibility, good mechanical strength, and large modifiable surface area. Herein, a novel approach is taken by using functionalized BNC as a substrate to build a molecularly imprinted photonic sensing layer. BNC was modified with polydopamine (PDA), which improved the adhesion and mechanical properties of the BNC substrate while providing simultaneously a black background for color saturation. A molecularly imprinted polymer (MIP) also made of PDA was used to create the recognition sites for the biomarker lysozyme. A monodisperse colloidal suspension of silica particles was first synthesized and used as core of the MIP shell, and then the photonic structure was assembled on the PDA-BNC membrane. The biosensor showed a detection limit of about 0.8nmolL1 of lysozyme in spiked human serum and demonstrated to be selective against cystatin C. These properties, combined with biocompatible, eco-friendly, and low-cost materials, offer a sustainable sensing platform with great potential for healthcare applications. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05 2023-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/82950 |
url |
https://hdl.handle.net/1822/82950 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Suleimenova, Akmaral; Frasco, Manuela; Soares da Silva, F. A. G.; Gama, F. M.; Fortunato, Elvira; Sales, M. G. F., Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing. Biosensors and Bioelectronics: X, 13(100310), 2023 2590-1370 10.1016/j.biosx.2023.100310 https://www.sciencedirect.com/journal/biosensors-and-bioelectronics-x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132722586714112 |