In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/59019 |
Resumo: | This work reports on a large-scale potential neuropeptide activity screening in human gut microbiomes deposited in public databases. In our experimental approach, the sequences of the bioactive peptides collected in the MAHMI database, mainly predicted as immunomodulatory or antitumoral, were crossed with those of the neuroactive/digestive peptides. From 91,325,790 potential bioactive peptides, only 581 returned a match when crossed against the 5949 neuroactive peptides from the NeuroPep database and the 15 digestive hormones. Relevant bacterial taxa, such as Ruminococcus sp., Clostridium sp. were found among the main producers of the matching sequences, and many of the matches corresponded to adiponectin and the hormone produced by adipocites, which is involved in glucose homeostasis. These results show, for the first time, the presence of potentially bioactive peptides produced by gut microbiota members over the nervous cells, most notably, peptides with already predicted immunomodulatory or anti-inflammatory activity. Classical (Lactobacillus sp.) and next-generation (Faecalibacterium sp.) probiotics are shown to produce these peptides, which are proposed as a potential mechanism of action of psychobiotics. Our previous experimental results showed that many of these peptides were active when incubated with immune cells, such as dendritic cells, so their effect over the nervous system innervating the gut mucosa holds significant potential and should be explored. |
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In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiotaGut microbiotaBioactive peptidesMetaproteomesPeptidomesNeuropeptidesAnti-inflammatoryImmunomodulatoryMicrobiomeCiências Agrárias::Biotecnologia Agrária e AlimentarCiências Médicas::Biotecnologia MédicaEngenharia e Tecnologia::Biotecnologia IndustrialScience & TechnologyThis work reports on a large-scale potential neuropeptide activity screening in human gut microbiomes deposited in public databases. In our experimental approach, the sequences of the bioactive peptides collected in the MAHMI database, mainly predicted as immunomodulatory or antitumoral, were crossed with those of the neuroactive/digestive peptides. From 91,325,790 potential bioactive peptides, only 581 returned a match when crossed against the 5949 neuroactive peptides from the NeuroPep database and the 15 digestive hormones. Relevant bacterial taxa, such as Ruminococcus sp., Clostridium sp. were found among the main producers of the matching sequences, and many of the matches corresponded to adiponectin and the hormone produced by adipocites, which is involved in glucose homeostasis. These results show, for the first time, the presence of potentially bioactive peptides produced by gut microbiota members over the nervous cells, most notably, peptides with already predicted immunomodulatory or anti-inflammatory activity. Classical (Lactobacillus sp.) and next-generation (Faecalibacterium sp.) probiotics are shown to produce these peptides, which are proposed as a potential mechanism of action of psychobiotics. Our previous experimental results showed that many of these peptides were active when incubated with immune cells, such as dendritic cells, so their effect over the nervous system innervating the gut mucosa holds significant potential and should be explored.Spanish “Programa Estatal de Investigación, Desarrollo e Inovación Orientada a los Retos de la Sociedad” (Grant AGL2016-78311-R); the Asociación Española Contra el Cancer (“Obtención de péptidos bioactivos contra el Cáncer Colo-Rectal a partir de secuencias genéticas de microbiomas intestinales”, grant PS-2016). This study was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER006684). SING group thanks CITI (Centro de Investigación, Transferencia e Innovación) from University of Vigo for hosting its IT infrastructure. This work was partially supported by the Asturias Regional Plan I+D+i for research groups (FYCYT-IDI/2018/000236) and by the Consellería de Educación, Universidades e Formación Profesional (Xunta de Galicia) under the scope of the strategic funding of ED431C2018/55-GRC Competitive Reference Groupinfo:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoBlanco-Míguez, AitorFdez-Riverola, FlorentinoLourenço, AnáliaSánchez, Borja2019-052019-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/59019engBlanco-Míguez, Aitor; Fdez-Riverola, Florentino; Lourenço, Anália; Sánchez, Borja, In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota. Food Research International, 119, 221-226, 20190963-996910.1016/j.foodres.2019.01.06930884651https://www.sciencedirect.com/science/article/pii/S096399691930078Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:24Zoai:repositorium.sdum.uminho.pt:1822/59019Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:39.096576Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota |
title |
In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota |
spellingShingle |
In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota Blanco-Míguez, Aitor Gut microbiota Bioactive peptides Metaproteomes Peptidomes Neuropeptides Anti-inflammatory Immunomodulatory Microbiome Ciências Agrárias::Biotecnologia Agrária e Alimentar Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial Science & Technology |
title_short |
In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota |
title_full |
In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota |
title_fullStr |
In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota |
title_full_unstemmed |
In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota |
title_sort |
In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota |
author |
Blanco-Míguez, Aitor |
author_facet |
Blanco-Míguez, Aitor Fdez-Riverola, Florentino Lourenço, Anália Sánchez, Borja |
author_role |
author |
author2 |
Fdez-Riverola, Florentino Lourenço, Anália Sánchez, Borja |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Blanco-Míguez, Aitor Fdez-Riverola, Florentino Lourenço, Anália Sánchez, Borja |
dc.subject.por.fl_str_mv |
Gut microbiota Bioactive peptides Metaproteomes Peptidomes Neuropeptides Anti-inflammatory Immunomodulatory Microbiome Ciências Agrárias::Biotecnologia Agrária e Alimentar Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial Science & Technology |
topic |
Gut microbiota Bioactive peptides Metaproteomes Peptidomes Neuropeptides Anti-inflammatory Immunomodulatory Microbiome Ciências Agrárias::Biotecnologia Agrária e Alimentar Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial Science & Technology |
description |
This work reports on a large-scale potential neuropeptide activity screening in human gut microbiomes deposited in public databases. In our experimental approach, the sequences of the bioactive peptides collected in the MAHMI database, mainly predicted as immunomodulatory or antitumoral, were crossed with those of the neuroactive/digestive peptides. From 91,325,790 potential bioactive peptides, only 581 returned a match when crossed against the 5949 neuroactive peptides from the NeuroPep database and the 15 digestive hormones. Relevant bacterial taxa, such as Ruminococcus sp., Clostridium sp. were found among the main producers of the matching sequences, and many of the matches corresponded to adiponectin and the hormone produced by adipocites, which is involved in glucose homeostasis. These results show, for the first time, the presence of potentially bioactive peptides produced by gut microbiota members over the nervous cells, most notably, peptides with already predicted immunomodulatory or anti-inflammatory activity. Classical (Lactobacillus sp.) and next-generation (Faecalibacterium sp.) probiotics are shown to produce these peptides, which are proposed as a potential mechanism of action of psychobiotics. Our previous experimental results showed that many of these peptides were active when incubated with immune cells, such as dendritic cells, so their effect over the nervous system innervating the gut mucosa holds significant potential and should be explored. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05 2019-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/59019 |
url |
http://hdl.handle.net/1822/59019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Blanco-Míguez, Aitor; Fdez-Riverola, Florentino; Lourenço, Anália; Sánchez, Borja, In silico prediction reveals the existence of potential bioactive neuropeptides produced by the human gut microbiota. Food Research International, 119, 221-226, 2019 0963-9969 10.1016/j.foodres.2019.01.069 30884651 https://www.sciencedirect.com/science/article/pii/S096399691930078X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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