Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization

Detalhes bibliográficos
Autor(a) principal: Pista, Angela
Data de Publicação: 2022
Outros Autores: Silveira, Leonor, Ribeiro, Sofia, Fontes, Mariana, Castro, Rita, Coelho, Anabela, Furtado, Rosália, Lopes, Teresa, Maia, Carla, Mixão, Verónica, Borges, Vítor, Sá, Ana, Soeiro, Vanessa, Correia, Cristina Belo, Gomes, João Paulo, Saraiva, Margarida, Oleastro, Mónica, Batista, Rita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8430
Resumo: This article belongs to the Section Food Microbiology.
id RCAP_bbf27fa4f341c8ee221655a88c67aa13
oai_identifier_str oai:repositorio.insa.pt:10400.18/8430
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic CharacterizationPathogenic Escherichia coliSalmonella spp.Campylobacter spp.Whole Genome SequencingWild AnimalsPortugalSegurança AlimentarInfecções GastrointestinaisThis article belongs to the Section Food Microbiology.Human–wildlife coexistence may increase the potential risk of direct transmission of emergent or re-emergent zoonotic pathogens to humans. Intending to assess the occurrence of three important foodborne pathogens in wild animals of two wildlife conservation centers in Portugal, we investigated 132 fecal samples for the presence of Escherichia coli (Shiga toxin-producing E. coli (STEC) and non-STEC), Salmonella spp. and Campylobacter spp. A genotypic search for genes having virulence and antimicrobial resistance (AMR) was performed by means of PCR and Whole-Genome Sequencing (WGS) and phenotypic (serotyping and AMR profiles) characterization. Overall, 62 samples tested positive for at least one of these species: 27.3% for STEC, 11.4% for non-STEC, 3.0% for Salmonella spp. and 6.8% for Campylobacter spp. AMR was detected in four E. coli isolates and the only Campylobacter coli isolated in this study. WGS analysis revealed that 57.7% (30/52) of pathogenic E. coli integrated genetic clusters of highly closely related isolates (often involving different animal species), supporting the circulation and transmission of different pathogenic E. coli strains in the studied areas. These results support the idea that the health of humans, animals and ecosystems are interconnected, reinforcing the importance of a One Health approach to better monitor and control public health threats.This work was supported by funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 773830: One Health European Joint Pro- gramme, as part of the DiSCoVeR project (Discovering the sources of Salmonella, Campylobacter, VTEC and Antimicrobial Resistance). S.R., R.C. and V.M. were beneficiaries of fellowships from the same Programme on behalf of ADONIS (S.R.), FedAMR (R.C.) and BeOne (V.M) projects.MDPIRepositório Científico do Instituto Nacional de SaúdePista, AngelaSilveira, LeonorRibeiro, SofiaFontes, MarianaCastro, RitaCoelho, AnabelaFurtado, RosáliaLopes, TeresaMaia, CarlaMixão, VerónicaBorges, VítorSá, AnaSoeiro, VanessaCorreia, Cristina BeloGomes, João PauloSaraiva, MargaridaOleastro, MónicaBatista, Rita2023-01-12T10:34:55Z2022-10-172022-10-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8430engMicroorganisms . 2022 Oct 27;10(11):2132. doi: 10.3390/microorganisms101121322076-260710.3390/microorganisms10112132info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:31Zoai:repositorio.insa.pt:10400.18/8430Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:00.442385Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization
title Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization
spellingShingle Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization
Pista, Angela
Pathogenic Escherichia coli
Salmonella spp.
Campylobacter spp.
Whole Genome Sequencing
Wild Animals
Portugal
Segurança Alimentar
Infecções Gastrointestinais
title_short Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization
title_full Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization
title_fullStr Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization
title_full_unstemmed Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization
title_sort Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization
author Pista, Angela
author_facet Pista, Angela
Silveira, Leonor
Ribeiro, Sofia
Fontes, Mariana
Castro, Rita
Coelho, Anabela
Furtado, Rosália
Lopes, Teresa
Maia, Carla
Mixão, Verónica
Borges, Vítor
Sá, Ana
Soeiro, Vanessa
Correia, Cristina Belo
Gomes, João Paulo
Saraiva, Margarida
Oleastro, Mónica
Batista, Rita
author_role author
author2 Silveira, Leonor
Ribeiro, Sofia
Fontes, Mariana
Castro, Rita
Coelho, Anabela
Furtado, Rosália
Lopes, Teresa
Maia, Carla
Mixão, Verónica
Borges, Vítor
Sá, Ana
Soeiro, Vanessa
Correia, Cristina Belo
Gomes, João Paulo
Saraiva, Margarida
Oleastro, Mónica
Batista, Rita
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Pista, Angela
Silveira, Leonor
Ribeiro, Sofia
Fontes, Mariana
Castro, Rita
Coelho, Anabela
Furtado, Rosália
Lopes, Teresa
Maia, Carla
Mixão, Verónica
Borges, Vítor
Sá, Ana
Soeiro, Vanessa
Correia, Cristina Belo
Gomes, João Paulo
Saraiva, Margarida
Oleastro, Mónica
Batista, Rita
dc.subject.por.fl_str_mv Pathogenic Escherichia coli
Salmonella spp.
Campylobacter spp.
Whole Genome Sequencing
Wild Animals
Portugal
Segurança Alimentar
Infecções Gastrointestinais
topic Pathogenic Escherichia coli
Salmonella spp.
Campylobacter spp.
Whole Genome Sequencing
Wild Animals
Portugal
Segurança Alimentar
Infecções Gastrointestinais
description This article belongs to the Section Food Microbiology.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-17
2022-10-17T00:00:00Z
2023-01-12T10:34:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8430
url http://hdl.handle.net/10400.18/8430
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms . 2022 Oct 27;10(11):2132. doi: 10.3390/microorganisms10112132
2076-2607
10.3390/microorganisms10112132
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132175883304960

Registros relacionados