Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe Nucleus

Detalhes bibliográficos
Autor(a) principal: Turco, Katherine
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/24639
Resumo: Dissertação de mestrado em Biologia Celular e Molecular, apresentada ao Departamento Ciências da Vida da Faculdade de Ciências e Tecnologia da Universidade de Coimbra.
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spelling Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe NucleusSerotonin5-HTDorsal raphe nucleusPiriform cortexOlfactory bulbProjectionsRetrograde tracingRetrograde labelingHiRetCAV2Dissertação de mestrado em Biologia Celular e Molecular, apresentada ao Departamento Ciências da Vida da Faculdade de Ciências e Tecnologia da Universidade de Coimbra.The serotonin system is considered one of the most complex neuromodulatory systems in the central nervous system and is one of the most frequently targeted systems for pharmacological studies addressing a wide range of psychiatric dysfunctions. Serotonergic neurons are topographically organized with anatomically distinct groups receiving synaptic input from specific brain structures, suggesting organization in both function and projection output to a wide range of forebrain and brainstem circuits. There still remains much confusion about the role of serotonin, and many of the functions effected by serotonin modulation, including affective control, behavioral inhibition and sensorimotor gating, may be linked to the structural organization mapped within the system. The aim of this project is to develop and implement labeling methods to visualize and control specific projection pathways of serotonin neurons originating in the dorsal raphe nucleus. Retrograde labeling tools were explored and optimized. We were able to optimize the experimental protocol for retrograde labeling using latex microspheres, and the results confirmed that there are projections from the dorsal raphe nucleus to both the piriform cortex and the olfactory bulb with a majority of these neurons being serotonergic. Piriform cortex projecting cells were more numerous and clustered in the posterior portion of the dorsal raphe region. Two types of retrograde viruses were also tested, though with less success and efficiency. They do, however, offer future possibilities for research exploring the structural and functional anatomy of the serotonin system.2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10316/24639http://hdl.handle.net/10316/24639TID:201668653engTurco, Katherineinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-01-20T17:48:32Zoai:estudogeral.uc.pt:10316/24639Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:56:48.765251Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe Nucleus
title Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe Nucleus
spellingShingle Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe Nucleus
Turco, Katherine
Serotonin
5-HT
Dorsal raphe nucleus
Piriform cortex
Olfactory bulb
Projections
Retrograde tracing
Retrograde labeling
HiRet
CAV2
title_short Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe Nucleus
title_full Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe Nucleus
title_fullStr Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe Nucleus
title_full_unstemmed Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe Nucleus
title_sort Mapping the Serotonergic System : Topographical Organization of Serotonergic Projections from the Dorsal Raphe Nucleus
author Turco, Katherine
author_facet Turco, Katherine
author_role author
dc.contributor.author.fl_str_mv Turco, Katherine
dc.subject.por.fl_str_mv Serotonin
5-HT
Dorsal raphe nucleus
Piriform cortex
Olfactory bulb
Projections
Retrograde tracing
Retrograde labeling
HiRet
CAV2
topic Serotonin
5-HT
Dorsal raphe nucleus
Piriform cortex
Olfactory bulb
Projections
Retrograde tracing
Retrograde labeling
HiRet
CAV2
description Dissertação de mestrado em Biologia Celular e Molecular, apresentada ao Departamento Ciências da Vida da Faculdade de Ciências e Tecnologia da Universidade de Coimbra.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/24639
http://hdl.handle.net/10316/24639
TID:201668653
url http://hdl.handle.net/10316/24639
identifier_str_mv TID:201668653
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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