In vitro adsorption of aflatoxin B1, ochratoxin A, and zearalenone by micronized grape stems and olive pomace in buffer solutions

• Autor(a) principal: Fernandes, J. -M.
• Data de Publicação: 2019
• Outros Autores: Calado, Thalita, Guimarães, Ana Cristina Silva Esperança, Rodrigues, Miguel A. M., Abrunhosa, Luís
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/60843
• topic: Aflatoxin B1; Binders; Feed additives; Isotherms; Ochratoxin A; Zearalenone; Science & Technology

This work characterizes the adsorption of aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEN) by dry micronized olive pomace (OliPom) and grape stems (GrapStem). Their performance was compared with that of three other materials, activated carbon (ActCarb), bentonite (Bent), and a commercial product (ComProd). Experiments were conducted in vitro at several pH values using buffer solutions. For OTA and ZEA, the strongest adsorbent was ActCarb, with 5 mg/mL being sufficient to bind >99% of all the mycotoxins. For AFB1, ComProd and Bent were the most effective adsorbents, as 0.5 mg/mL bound >95% of this mycotoxin. Among the two agro by-products, GrapStem was the strongest binder, with 10 mg/mL being sufficient to bind at least 90% of all the mycotoxins (except OTA at pH 7). OliPom was the least efficient material, but at a concentration of 30 mg/mL, its performance was similar to GrapStem. Adsorption isotherms were calculated, and ActCarb showed the maximum adsorption capacity (Qmax), with values that ranged from 19 to 24 g/mg for pH 2 and from 17 to 20 g/mg for pH 7. ComProd, Bent, and GrapStem showed more similar Qmax between them (1.44.4 g/mg for pH 2 and 0.54.8 g/mg for pH 7).