A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites

Detalhes bibliográficos
Autor(a) principal: Oliveira, Maria João
Data de Publicação: 2023
Outros Autores: Caetano, Soraia, Dalot, Ana, Sabino, Filipe, Calmeiro, Tomás R., Fortunato, Elvira, Martins, Rodrigo, Pereira, Eulália, Prudêncio, Miguel, Byrne, Hugh J., Franco, Ricardo, Águas, Hugo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/159670
Resumo: M. P. acknowledges “la Caixa” Foundation for Grant HR21-848. Publisher Copyright: © 2023 The Royal Society of Chemistry.
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spelling A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasitesAnalytical ChemistryBiochemistryEnvironmental ChemistrySpectroscopyElectrochemistrySDG 3 - Good Health and Well-beingM. P. acknowledges “la Caixa” Foundation for Grant HR21-848. Publisher Copyright: © 2023 The Royal Society of Chemistry.Early and accurate detection of infection by pathogenic microorganisms, such as Plasmodium, the causative agent of malaria, is critical for clinical diagnosis and ultimately determines the patient's outcome. We have combined a polystyrene-based microfluidic device with an immunoassay which utilises Surface-Enhanced Raman Spectroscopy (SERS) to detect malaria. The method can be easily translated to a point-of-care testing format and shows excellent sensitivity and specificity, when compared to the gold standard for laboratorial detection of Plasmodium infections. The device can be fabricated in less than 30 min by direct patterning on shrinkable polystyrene sheets of adaptable three-dimensional microfluidic chips. To validate the microfluidic system, samples of P. falciparum-infected red blood cell cultures were used. The SERS-based immunoassay enabled the detection of 0.0012 ± 0.0001% parasitaemia in a P. falciparum-infected red blood cell culture supernatant, an ∼7-fold higher sensitivity than that attained by most rapid diagnostic tests. Our approach successfully overcomes the main challenges of the current Plasmodium detection methods, including increased reproducibility, sensitivity, and specificity. Furthermore, our system can be easily adapted for detection of other pathogens and has excellent properties for early diagnosis of infectious diseases, a decisive step towards lowering their high burden on healthcare systems worldwide.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisUCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaUNINOVA-Instituto de Desenvolvimento de Novas TecnologiasRUNOliveira, Maria JoãoCaetano, SoraiaDalot, AnaSabino, FilipeCalmeiro, Tomás R.Fortunato, ElviraMartins, RodrigoPereira, EuláliaPrudêncio, MiguelByrne, Hugh J.Franco, RicardoÁguas, Hugo2023-11-07T22:08:54Z2023-08-212023-08-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/159670eng0003-2654PURE: 75585659https://doi.org/10.1039/d3an00971hinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-18T01:43:42Zoai:run.unl.pt:10362/159670Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:38.651676Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites
title A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites
spellingShingle A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites
Oliveira, Maria João
Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry
SDG 3 - Good Health and Well-being
title_short A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites
title_full A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites
title_fullStr A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites
title_full_unstemmed A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites
title_sort A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites
author Oliveira, Maria João
author_facet Oliveira, Maria João
Caetano, Soraia
Dalot, Ana
Sabino, Filipe
Calmeiro, Tomás R.
Fortunato, Elvira
Martins, Rodrigo
Pereira, Eulália
Prudêncio, Miguel
Byrne, Hugh J.
Franco, Ricardo
Águas, Hugo
author_role author
author2 Caetano, Soraia
Dalot, Ana
Sabino, Filipe
Calmeiro, Tomás R.
Fortunato, Elvira
Martins, Rodrigo
Pereira, Eulália
Prudêncio, Miguel
Byrne, Hugh J.
Franco, Ricardo
Águas, Hugo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCM - Departamento de Ciência dos Materiais
UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
RUN
dc.contributor.author.fl_str_mv Oliveira, Maria João
Caetano, Soraia
Dalot, Ana
Sabino, Filipe
Calmeiro, Tomás R.
Fortunato, Elvira
Martins, Rodrigo
Pereira, Eulália
Prudêncio, Miguel
Byrne, Hugh J.
Franco, Ricardo
Águas, Hugo
dc.subject.por.fl_str_mv Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry
SDG 3 - Good Health and Well-being
topic Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry
SDG 3 - Good Health and Well-being
description M. P. acknowledges “la Caixa” Foundation for Grant HR21-848. Publisher Copyright: © 2023 The Royal Society of Chemistry.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-07T22:08:54Z
2023-08-21
2023-08-21T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/159670
url http://hdl.handle.net/10362/159670
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0003-2654
PURE: 75585659
https://doi.org/10.1039/d3an00971h
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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