DNA gel particles: an overview

• Autor(a) principal: Morán, M. Carmen
• Data de Publicação: 2014
• Outros Autores: Vinardell, M. Pilar, Infante, M. Rosa, Miguel, M. Graça, Lindman, Björn
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10316/27384; https://doi.org/10.1016/j.cis.2013.09.002
• topic: DNA gels; DNA–oppositely charged agent interaction; Particles; DNA release; Hemocompatibility; Cytotoxicity

A general understanding of interactions between DNA and oppositely charged compounds forms the basis for developing novel DNA-based materials, including gel particles. The association strength, which is altered by varying the chemical structure of the cationic cosolute, determines the spatial homogeneity of the gelation process, creating DNA reservoir devices and DNA matrix devices that can be designed to release either single- (ssDNA) or double-stranded (dsDNA) DNA. This review covers recent developments on the topic of DNA gel particles formed in water–water emulsion-type interfaces. The degree of DNA entrapment, particle morphology, swelling/dissolution behavior and DNA release responses are discussed as functions of the nature of the cationic agent used. On the basis of designing DNA gel particles for therapeutic purposes, recent studies on the determination of the surface hydrophobicity and the hemolytic and the cytotoxic assessments of the obtained DNA gel particles have been also reported.