Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress

Detalhes bibliográficos
Autor(a) principal: Oliveira, Ana S.
Data de Publicação: 2023
Outros Autores: Saraiva, Lígia M., Carvalho, Sandra M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158524
Resumo: Funding Information: This work was financially supported by Fundação para a Ciência e Tecnologia (FCT) Project – PTDC/BIA-MIC/31566/2017, R&D unit MOSTMICRO-ITQB (UIDB/04612/2020 and UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). AO has a fellowship UI/BD/153389/2022 from FCT. The NMR data were acquired from CERMAX, ITQB-NOVA, Oeiras, Portugal, with equipment funded by FCT, project AAC 01/SAICT/2016. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgments Publisher Copyright: Copyright © 2023 Oliveira, Saraiva and Carvalho.
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spelling Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stressbiofilm metabolismconfocal laser scanning microscopy (CLSM)nitrosative stressnuclear magnetic resonance (NMR)Staphylococcus epidermidisMicrobiologyImmunologyMicrobiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: This work was financially supported by Fundação para a Ciência e Tecnologia (FCT) Project – PTDC/BIA-MIC/31566/2017, R&D unit MOSTMICRO-ITQB (UIDB/04612/2020 and UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). AO has a fellowship UI/BD/153389/2022 from FCT. The NMR data were acquired from CERMAX, ITQB-NOVA, Oeiras, Portugal, with equipment funded by FCT, project AAC 01/SAICT/2016. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgments Publisher Copyright: Copyright © 2023 Oliveira, Saraiva and Carvalho.Staphylococcus epidermidis is a commensal skin bacterium that forms host- and antibiotic-resistant biofilms that are a major cause of implant-associated infections. Most research has focused on studying the responses to host-imposed stresses on planktonic bacteria. In this work, we addressed the open question of how S. epidermidis thrives on toxic concentrations of nitric oxide (NO) produced by host innate immune cells during biofilm assembly. We analyzed alterations of gene expression, metabolism, and matrix structure of biofilms of two clinical isolates of S. epidermidis, namely, 1457 and RP62A, formed under NO stress conditions. In both strains, NO lowers the amount of biofilm mass and causes increased production of lactate and decreased acetate excretion from biofilm glucose metabolism. Transcriptional analysis revealed that NO induces icaA, which is directly involved in polysaccharide intercellular adhesion (PIA) production, and genes encoding proteins of the amino sugar pathway (glmM and glmU) that link glycolysis to PIA synthesis. However, the strains seem to have distinct regulatory mechanisms to boost lactate production, as NO causes a substantial upregulation of ldh gene in strain RP62A but not in strain 1457. The analysis of the matrix components of the staphylococcal biofilms, assessed by confocal laser scanning microscopy (CLSM), showed that NO stimulates PIA and protein production and interferes with biofilm structure in a strain-dependent manner, but independently of the Ldh level. Thus, NO resistance is attained by remodeling the staphylococcal matrix architecture and adaptation of main metabolic processes, likely providing in vivo fitness of S. epidermidis biofilms contacting NO-proficient macrophages.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNOliveira, Ana S.Saraiva, Lígia M.Carvalho, Sandra M.2023-09-30T22:18:50Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158524eng2235-2988PURE: 72373157https://doi.org/10.3389/fcimb.2023.1200923info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:00Zoai:run.unl.pt:10362/158524Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:11.258326Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress
title Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress
spellingShingle Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress
Oliveira, Ana S.
biofilm metabolism
confocal laser scanning microscopy (CLSM)
nitrosative stress
nuclear magnetic resonance (NMR)
Staphylococcus epidermidis
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress
title_full Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress
title_fullStr Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress
title_full_unstemmed Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress
title_sort Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress
author Oliveira, Ana S.
author_facet Oliveira, Ana S.
Saraiva, Lígia M.
Carvalho, Sandra M.
author_role author
author2 Saraiva, Lígia M.
Carvalho, Sandra M.
author2_role author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Oliveira, Ana S.
Saraiva, Lígia M.
Carvalho, Sandra M.
dc.subject.por.fl_str_mv biofilm metabolism
confocal laser scanning microscopy (CLSM)
nitrosative stress
nuclear magnetic resonance (NMR)
Staphylococcus epidermidis
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
topic biofilm metabolism
confocal laser scanning microscopy (CLSM)
nitrosative stress
nuclear magnetic resonance (NMR)
Staphylococcus epidermidis
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding Information: This work was financially supported by Fundação para a Ciência e Tecnologia (FCT) Project – PTDC/BIA-MIC/31566/2017, R&D unit MOSTMICRO-ITQB (UIDB/04612/2020 and UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). AO has a fellowship UI/BD/153389/2022 from FCT. The NMR data were acquired from CERMAX, ITQB-NOVA, Oeiras, Portugal, with equipment funded by FCT, project AAC 01/SAICT/2016. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgments Publisher Copyright: Copyright © 2023 Oliveira, Saraiva and Carvalho.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:18:50Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158524
url http://hdl.handle.net/10362/158524
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2235-2988
PURE: 72373157
https://doi.org/10.3389/fcimb.2023.1200923
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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