An NMR structural study of nickel-substituted rubredoxin

Detalhes bibliográficos
Autor(a) principal: Goodfellow, Brian J.
Data de Publicação: 2010
Outros Autores: Duarte, Iven C. N., Macedo, Anjos L., Volkman, Brian F., Nunes, Sofia G., Moura, Isabel, Markley, John L., Moura, José J. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8630
Resumo: J Biol Inorg Chem (2010) 15:409–420 DOI 10.1007/s00775-009-0613-6
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spelling An NMR structural study of nickel-substituted rubredoxinNMRRubredoxin[Fe–4S] centreParamagnetic proteinNickelJ Biol Inorg Chem (2010) 15:409–420 DOI 10.1007/s00775-009-0613-6The Ni(II) and Zn(II) derivatives of Desulfovibrio vulgaris rubredoxin (DvRd) have been studied by NMR spectroscopy to probe the structure at the metal centre. The βCH2 proton pairs from the cysteines that bind the Ni(II) atom have been identified using 1D nuclear Overhauser enhancement (NOE) difference spectra and sequence specifically assigned via NOE correlations to neighbouring protons and by comparison with the published X-ray crystal structure of a Ni(II) derivative of Clostridium pasteurianum rubredoxin. The solution structures of DvRd(Zn) and DvRd(Ni) have been determined and the paramagnetic form refined using pseudocontact shifts. The determination of the magnetic susceptibility anisotropy tensor allowed the contact and pseudocontact contributions to the observed chemical shifts to be obtained. Analysis of the pseudocontact and contact chemical shifts of the cysteine Hβ protons and backbone protons close to the metal centre allowed conclusions to be drawn as to the geometry and hydrogen-bonding pattern at the metal binding site. The importance of NH–S hydrogen bonds at the metal centre for the delocalization of electron spin density is confirmed for rubredoxins and can be extrapolated to metal centres in Cu proteins: amicyanin, plastocyanin, stellacyanin, azurin and pseudoazurin.SpringerRUNGoodfellow, Brian J.Duarte, Iven C. N.Macedo, Anjos L.Volkman, Brian F.Nunes, Sofia G.Moura, IsabelMarkley, John L.Moura, José J. G.2013-01-30T16:01:50Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8630eng1432-1327info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:27Zoai:run.unl.pt:10362/8630Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:19.941010Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An NMR structural study of nickel-substituted rubredoxin
title An NMR structural study of nickel-substituted rubredoxin
spellingShingle An NMR structural study of nickel-substituted rubredoxin
Goodfellow, Brian J.
NMR
Rubredoxin
[Fe–4S] centre
Paramagnetic protein
Nickel
title_short An NMR structural study of nickel-substituted rubredoxin
title_full An NMR structural study of nickel-substituted rubredoxin
title_fullStr An NMR structural study of nickel-substituted rubredoxin
title_full_unstemmed An NMR structural study of nickel-substituted rubredoxin
title_sort An NMR structural study of nickel-substituted rubredoxin
author Goodfellow, Brian J.
author_facet Goodfellow, Brian J.
Duarte, Iven C. N.
Macedo, Anjos L.
Volkman, Brian F.
Nunes, Sofia G.
Moura, Isabel
Markley, John L.
Moura, José J. G.
author_role author
author2 Duarte, Iven C. N.
Macedo, Anjos L.
Volkman, Brian F.
Nunes, Sofia G.
Moura, Isabel
Markley, John L.
Moura, José J. G.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Goodfellow, Brian J.
Duarte, Iven C. N.
Macedo, Anjos L.
Volkman, Brian F.
Nunes, Sofia G.
Moura, Isabel
Markley, John L.
Moura, José J. G.
dc.subject.por.fl_str_mv NMR
Rubredoxin
[Fe–4S] centre
Paramagnetic protein
Nickel
topic NMR
Rubredoxin
[Fe–4S] centre
Paramagnetic protein
Nickel
description J Biol Inorg Chem (2010) 15:409–420 DOI 10.1007/s00775-009-0613-6
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2013-01-30T16:01:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8630
url http://hdl.handle.net/10362/8630
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1432-1327
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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