Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491

Detalhes bibliográficos
Autor(a) principal: Mota, Cristiano S.
Data de Publicação: 2011
Outros Autores: Valette, Odile, J. González, Pablo, Brondino, Carlos D., Moura, José J. G., Moura, Isabel, Dolla, Alain, Rivas, Maria G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/6968
Resumo: Journal of Bacteriology. 2011 Jun; Vol. 193 issue 12 pages 2917-2923
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spelling Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491Journal of Bacteriology. 2011 Jun; Vol. 193 issue 12 pages 2917-2923Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 M molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 M tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.American Society for MicrobiologyRUNMota, Cristiano S.Valette, OdileJ. González, PabloBrondino, Carlos D.Moura, José J. G.Moura, IsabelDolla, AlainRivas, Maria G.2012-02-08T16:19:41Z2011-042011-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/6968eng0021-9193info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:38:28Zoai:run.unl.pt:10362/6968Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:17:07.594927Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491
title Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491
spellingShingle Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491
Mota, Cristiano S.
title_short Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491
title_full Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491
title_fullStr Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491
title_full_unstemmed Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491
title_sort Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by desulfovibrio alaskensis NCIMB 13491
author Mota, Cristiano S.
author_facet Mota, Cristiano S.
Valette, Odile
J. González, Pablo
Brondino, Carlos D.
Moura, José J. G.
Moura, Isabel
Dolla, Alain
Rivas, Maria G.
author_role author
author2 Valette, Odile
J. González, Pablo
Brondino, Carlos D.
Moura, José J. G.
Moura, Isabel
Dolla, Alain
Rivas, Maria G.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Mota, Cristiano S.
Valette, Odile
J. González, Pablo
Brondino, Carlos D.
Moura, José J. G.
Moura, Isabel
Dolla, Alain
Rivas, Maria G.
description Journal of Bacteriology. 2011 Jun; Vol. 193 issue 12 pages 2917-2923
publishDate 2011
dc.date.none.fl_str_mv 2011-04
2011-04-01T00:00:00Z
2012-02-08T16:19:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/6968
url http://hdl.handle.net/10362/6968
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9193
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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