Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254

Detalhes bibliográficos
Autor(a) principal: López-Cortés, Alejandro
Data de Publicação: 2005
Outros Autores: Bursakov, Sergey, Figueiredo, Angelo, Thapper, Anders E., Todorovic, Smilja, Moura, José J. G., Ollivier, Bernard, Moura, Isabel, Fauque, Guy
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/7028
Resumo: Bioinorganic Chemistry and Applications Volume 3 (2005), Issue 1-2, Pages 81-91
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spelling Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254Bioinorganic Chemistry and Applications Volume 3 (2005), Issue 1-2, Pages 81-91Two proteins were purified and preliminarily characterized from the soluble extract of cells (310 g, wet weight) of the aminolytic and peptidolytic sulfate-reducing bacterium Desulfovibrio (D.) aminophilus DSM 12254. The iron-sulfur flavoenzyme adenylylsulfate (adenosine 5"-phosphosulfate, APS)reductase, a key enzyme in the microbial dissimilatory sulfate reduction, has been purified in three chromatographic steps(DEAE-Biogel A, Source 15, and Superdex 200 columns). It contains two different subunits with molecular masses of 75 and 18 kDa. The fraction after the last purification step had a purity index (A278 ,m/A388 nm) of 5.34, which was used for further EPR spectroscopic studies. The D. aminophilus APS reductase is very similar to the homologous enzymes isolated from D. gigas and D. desulfuricans ATCC 27774. A tetraheme cytochrome c3 (His-heme iron-His) has been purified in three chromatographic steps (DEAE- Biogel A,Source 15, and Biogel-HTP columns) and preliminarily characterized. It has a purity index ([A.s.s3 nm" A570 nm]rcd/m280nm ox) of 2.9 and a molecular mass of around 15 kDa, and its spectroscopic characterization(NMR and EPR) has been carried out. This hemoprotein presents similarities with the tetraheme cytochrome c3 from Desulfomicrobium (Des.) norvegicurn (NMR spectra, and N-terminal amino acid sequence).Hindawi Publishing CorporationRUNLópez-Cortés, AlejandroBursakov, SergeyFigueiredo, AngeloThapper, Anders E.Todorovic, SmiljaMoura, José J. G.Ollivier, BernardMoura, IsabelFauque, Guy2012-02-13T16:52:28Z20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/7028eng1565-3633info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:38:34Zoai:run.unl.pt:10362/7028Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:17:10.290512Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
title Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
spellingShingle Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
López-Cortés, Alejandro
title_short Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
title_full Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
title_fullStr Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
title_full_unstemmed Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
title_sort Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
author López-Cortés, Alejandro
author_facet López-Cortés, Alejandro
Bursakov, Sergey
Figueiredo, Angelo
Thapper, Anders E.
Todorovic, Smilja
Moura, José J. G.
Ollivier, Bernard
Moura, Isabel
Fauque, Guy
author_role author
author2 Bursakov, Sergey
Figueiredo, Angelo
Thapper, Anders E.
Todorovic, Smilja
Moura, José J. G.
Ollivier, Bernard
Moura, Isabel
Fauque, Guy
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv López-Cortés, Alejandro
Bursakov, Sergey
Figueiredo, Angelo
Thapper, Anders E.
Todorovic, Smilja
Moura, José J. G.
Ollivier, Bernard
Moura, Isabel
Fauque, Guy
description Bioinorganic Chemistry and Applications Volume 3 (2005), Issue 1-2, Pages 81-91
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
2012-02-13T16:52:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/7028
url http://hdl.handle.net/10362/7028
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dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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