Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensor
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042010000100003 |
Resumo: | Myeloperoxidase (MPO) is a neutrophil enzyme that employs hydrogen peroxide (H2O2) to catalyze the oxidation of halides and thiocyanate to their respective hypohalous acids. In this study, the inhibitory effect of nitrite (NO2-) on MPO-catalytic activity was investigated electrochemically. H2O2 consumption during steady-state catalysis was monitored amperometrically by a carbon fiber based H2O2-biosensor at 25 ºC. Optimized initial concentrations were 50 nM MPO, 10 μM H2O2, and a selected halide or thiocyanate concentration from physiological range. Under these conditions, reactions were monophasic and rapid (complete H2O2 consumption occurs in < 10 s). As concentration of NO2- increases, reactions change to biphasic (rapid step followed by a slow step) and both steps have been inhibited by NO2-. Our results confirmed the inhibitory effect of NO2- and demonstrated for the first time that NO2- is a strong inhibitor towards MPO-catalyzed oxidation of iodide and bromide; and a weak inhibitor towards MPO-catalyzed oxidation of chloride and thiocyanate. |
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Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensornitric oxidenitritemyeloperoxidasecatalytic activityH2O2-biosensorMyeloperoxidase (MPO) is a neutrophil enzyme that employs hydrogen peroxide (H2O2) to catalyze the oxidation of halides and thiocyanate to their respective hypohalous acids. In this study, the inhibitory effect of nitrite (NO2-) on MPO-catalytic activity was investigated electrochemically. H2O2 consumption during steady-state catalysis was monitored amperometrically by a carbon fiber based H2O2-biosensor at 25 ºC. Optimized initial concentrations were 50 nM MPO, 10 μM H2O2, and a selected halide or thiocyanate concentration from physiological range. Under these conditions, reactions were monophasic and rapid (complete H2O2 consumption occurs in < 10 s). As concentration of NO2- increases, reactions change to biphasic (rapid step followed by a slow step) and both steps have been inhibited by NO2-. Our results confirmed the inhibitory effect of NO2- and demonstrated for the first time that NO2- is a strong inhibitor towards MPO-catalyzed oxidation of iodide and bromide; and a weak inhibitor towards MPO-catalyzed oxidation of chloride and thiocyanate.Sociedade Portuguesa de Electroquímica2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042010000100003Portugaliae Electrochimica Acta v.28 n.1 2010reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042010000100003Tahboub,Yahya R.Abu-Soud,Husam M.info:eu-repo/semantics/openAccess2024-02-06T17:07:03Zoai:scielo:S0872-19042010000100003Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:20:08.426222Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensor |
title |
Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensor |
spellingShingle |
Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensor Tahboub,Yahya R. nitric oxide nitrite myeloperoxidase catalytic activity H2O2-biosensor |
title_short |
Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensor |
title_full |
Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensor |
title_fullStr |
Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensor |
title_full_unstemmed |
Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensor |
title_sort |
Steady-State Study of Inhibitory Effect of Nitrite on Myeloperoxidase Catalytic Activity by Hydrogen Peroxide Biosensor |
author |
Tahboub,Yahya R. |
author_facet |
Tahboub,Yahya R. Abu-Soud,Husam M. |
author_role |
author |
author2 |
Abu-Soud,Husam M. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Tahboub,Yahya R. Abu-Soud,Husam M. |
dc.subject.por.fl_str_mv |
nitric oxide nitrite myeloperoxidase catalytic activity H2O2-biosensor |
topic |
nitric oxide nitrite myeloperoxidase catalytic activity H2O2-biosensor |
description |
Myeloperoxidase (MPO) is a neutrophil enzyme that employs hydrogen peroxide (H2O2) to catalyze the oxidation of halides and thiocyanate to their respective hypohalous acids. In this study, the inhibitory effect of nitrite (NO2-) on MPO-catalytic activity was investigated electrochemically. H2O2 consumption during steady-state catalysis was monitored amperometrically by a carbon fiber based H2O2-biosensor at 25 ºC. Optimized initial concentrations were 50 nM MPO, 10 μM H2O2, and a selected halide or thiocyanate concentration from physiological range. Under these conditions, reactions were monophasic and rapid (complete H2O2 consumption occurs in < 10 s). As concentration of NO2- increases, reactions change to biphasic (rapid step followed by a slow step) and both steps have been inhibited by NO2-. Our results confirmed the inhibitory effect of NO2- and demonstrated for the first time that NO2- is a strong inhibitor towards MPO-catalyzed oxidation of iodide and bromide; and a weak inhibitor towards MPO-catalyzed oxidation of chloride and thiocyanate. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042010000100003 |
url |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042010000100003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042010000100003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Electroquímica |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Electroquímica |
dc.source.none.fl_str_mv |
Portugaliae Electrochimica Acta v.28 n.1 2010 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799137290360979456 |