Upscaling and consistent sialylation of plant produced antibodies
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/10930 |
Resumo: | Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Upscaling and consistent sialylation of plant produced antibodiesNicotiana benthamianaglycoengineeringbiopharmaceutical productsantibodiesRNAiMestrado em Engenharia Agronómica - Instituto Superior de AgronomiaGlycoproteins are a major part of biopharmaceutical products. For their production, a high level of homogeny is required, not only for the protein but also for attached glycans, since these oligossacharides influence protein function. For this goal, plants emerge as an ideal biopharmaceutical production platform. By infiltrating adult plant leaves with a suspension of Agrobacterium tumefaciens containing a plasmid for the gene of interest, a high amount of protein can be produced in a short period. In this study, the production of two antibodies in genetically glycoengineered Nicotiana benthamiana was described. These antibodies, Rituximab and KBPA, served as models for Immunoglobulin groups G and M. Additionally, co-infiltration of the genes for these antibodies with glycoengineering constructs allowed for specific glycan structures attached to these proteins. Finally, the down-regulation of β-N-acetylhexosaminidase 3 with RNAi technology was described, inhibiting the effect of this glycosidase on plant produced proteins.ISA/ULViegas, WandaSteinkellner, HertaCastilho, AlexandraRepositório da Universidade de LisboaSádio, Flávio da Silva Amaro2016-02-11T15:09:26Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/10930TID:201921863engSádio, F.S.A. - Upscaling and consistent sialylation of plant produced antibodies. Lisboa: ISA, 2015, 70 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:41:13Zoai:www.repository.utl.pt:10400.5/10930Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:57:18.609966Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Upscaling and consistent sialylation of plant produced antibodies |
title |
Upscaling and consistent sialylation of plant produced antibodies |
spellingShingle |
Upscaling and consistent sialylation of plant produced antibodies Sádio, Flávio da Silva Amaro Nicotiana benthamiana glycoengineering biopharmaceutical products antibodies RNAi |
title_short |
Upscaling and consistent sialylation of plant produced antibodies |
title_full |
Upscaling and consistent sialylation of plant produced antibodies |
title_fullStr |
Upscaling and consistent sialylation of plant produced antibodies |
title_full_unstemmed |
Upscaling and consistent sialylation of plant produced antibodies |
title_sort |
Upscaling and consistent sialylation of plant produced antibodies |
author |
Sádio, Flávio da Silva Amaro |
author_facet |
Sádio, Flávio da Silva Amaro |
author_role |
author |
dc.contributor.none.fl_str_mv |
Viegas, Wanda Steinkellner, Herta Castilho, Alexandra Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Sádio, Flávio da Silva Amaro |
dc.subject.por.fl_str_mv |
Nicotiana benthamiana glycoengineering biopharmaceutical products antibodies RNAi |
topic |
Nicotiana benthamiana glycoengineering biopharmaceutical products antibodies RNAi |
description |
Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2016-02-11T15:09:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/10930 TID:201921863 |
url |
http://hdl.handle.net/10400.5/10930 |
identifier_str_mv |
TID:201921863 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sádio, F.S.A. - Upscaling and consistent sialylation of plant produced antibodies. Lisboa: ISA, 2015, 70 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ISA/UL |
publisher.none.fl_str_mv |
ISA/UL |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799131056288301056 |