Upscaling and consistent sialylation of plant produced antibodies

Detalhes bibliográficos
Autor(a) principal: Sádio, Flávio da Silva Amaro
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/10930
Resumo: Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
id RCAP_cd0e65768e1250376c159db1d96a3391
oai_identifier_str oai:www.repository.utl.pt:10400.5/10930
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Upscaling and consistent sialylation of plant produced antibodiesNicotiana benthamianaglycoengineeringbiopharmaceutical productsantibodiesRNAiMestrado em Engenharia Agronómica - Instituto Superior de AgronomiaGlycoproteins are a major part of biopharmaceutical products. For their production, a high level of homogeny is required, not only for the protein but also for attached glycans, since these oligossacharides influence protein function. For this goal, plants emerge as an ideal biopharmaceutical production platform. By infiltrating adult plant leaves with a suspension of Agrobacterium tumefaciens containing a plasmid for the gene of interest, a high amount of protein can be produced in a short period. In this study, the production of two antibodies in genetically glycoengineered Nicotiana benthamiana was described. These antibodies, Rituximab and KBPA, served as models for Immunoglobulin groups G and M. Additionally, co-infiltration of the genes for these antibodies with glycoengineering constructs allowed for specific glycan structures attached to these proteins. Finally, the down-regulation of β-N-acetylhexosaminidase 3 with RNAi technology was described, inhibiting the effect of this glycosidase on plant produced proteins.ISA/ULViegas, WandaSteinkellner, HertaCastilho, AlexandraRepositório da Universidade de LisboaSádio, Flávio da Silva Amaro2016-02-11T15:09:26Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/10930TID:201921863engSádio, F.S.A. - Upscaling and consistent sialylation of plant produced antibodies. Lisboa: ISA, 2015, 70 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:41:13Zoai:www.repository.utl.pt:10400.5/10930Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:57:18.609966Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Upscaling and consistent sialylation of plant produced antibodies
title Upscaling and consistent sialylation of plant produced antibodies
spellingShingle Upscaling and consistent sialylation of plant produced antibodies
Sádio, Flávio da Silva Amaro
Nicotiana benthamiana
glycoengineering
biopharmaceutical products
antibodies
RNAi
title_short Upscaling and consistent sialylation of plant produced antibodies
title_full Upscaling and consistent sialylation of plant produced antibodies
title_fullStr Upscaling and consistent sialylation of plant produced antibodies
title_full_unstemmed Upscaling and consistent sialylation of plant produced antibodies
title_sort Upscaling and consistent sialylation of plant produced antibodies
author Sádio, Flávio da Silva Amaro
author_facet Sádio, Flávio da Silva Amaro
author_role author
dc.contributor.none.fl_str_mv Viegas, Wanda
Steinkellner, Herta
Castilho, Alexandra
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Sádio, Flávio da Silva Amaro
dc.subject.por.fl_str_mv Nicotiana benthamiana
glycoengineering
biopharmaceutical products
antibodies
RNAi
topic Nicotiana benthamiana
glycoengineering
biopharmaceutical products
antibodies
RNAi
description Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2016-02-11T15:09:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/10930
TID:201921863
url http://hdl.handle.net/10400.5/10930
identifier_str_mv TID:201921863
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sádio, F.S.A. - Upscaling and consistent sialylation of plant produced antibodies. Lisboa: ISA, 2015, 70 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA/UL
publisher.none.fl_str_mv ISA/UL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131056288301056

Registros relacionados