Control of flow-generated biofilms with surfactants : evidence of resistance and recovery
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/6061 |
Resumo: | The action of cetyltrimethyl ammonium bromide (CTAB) and sodium dodecyl sulphate (SDS), a cationic and an anionic surfactant respectively, were investigated for their ability to control turbulent and laminar flow-generated biofilms formed by P. fluorescens. The disinfectant action of CTAB and SDS on biofilms was assessed by respiratory activity, variation of mass and structure, immediately, 3, 7 and 12 h after the application of the surfactants. Laminar flow-generated biofilms were more susceptible to the action of CTAB than those formed under turbulent flow. Total inactivation of the cells within the biofilms was not achieved for either type of flow- enerated biofilms. For SDS, higher concentrations promoted significant biofilm inactivation, for both turbulent and laminar flow-generated biofilms. CTAB and SDS application did not promote the detachment of biofilms from the surfaces. Post-surfactant treatment, biofilms recovered respiratory activity, in some cases, reaching values higher than those found without chemical treatment. After CTAB treatment, the recovery of respiratory activity was not affected by the hydrodynamic conditions. Conversely, turbulent flow-generated biofilms showed a higher potential to recover their metabolic activity than laminar flow-generated biofilms, when previously challenged with SDS. Concerning biofilm mass, no significant variation (increase or decrease) was detected after 12 h of surfactant treatment. This study shows that care is needed when selecting the correct procedure and agent for biofilm control and demonstrates the influence of hydrodynamic conditions on the persistent and recalcitrant properties of P. fluorescens biofilms. |
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Control of flow-generated biofilms with surfactants : evidence of resistance and recoveryBiofilm controlHydrodynamic conditionsRecoveryResistanceSurfactantScience & TechnologyThe action of cetyltrimethyl ammonium bromide (CTAB) and sodium dodecyl sulphate (SDS), a cationic and an anionic surfactant respectively, were investigated for their ability to control turbulent and laminar flow-generated biofilms formed by P. fluorescens. The disinfectant action of CTAB and SDS on biofilms was assessed by respiratory activity, variation of mass and structure, immediately, 3, 7 and 12 h after the application of the surfactants. Laminar flow-generated biofilms were more susceptible to the action of CTAB than those formed under turbulent flow. Total inactivation of the cells within the biofilms was not achieved for either type of flow- enerated biofilms. For SDS, higher concentrations promoted significant biofilm inactivation, for both turbulent and laminar flow-generated biofilms. CTAB and SDS application did not promote the detachment of biofilms from the surfaces. Post-surfactant treatment, biofilms recovered respiratory activity, in some cases, reaching values higher than those found without chemical treatment. After CTAB treatment, the recovery of respiratory activity was not affected by the hydrodynamic conditions. Conversely, turbulent flow-generated biofilms showed a higher potential to recover their metabolic activity than laminar flow-generated biofilms, when previously challenged with SDS. Concerning biofilm mass, no significant variation (increase or decrease) was detected after 12 h of surfactant treatment. This study shows that care is needed when selecting the correct procedure and agent for biofilm control and demonstrates the influence of hydrodynamic conditions on the persistent and recalcitrant properties of P. fluorescens biofilms.Fundação para a Ciência e a Tecnologia (FCT)Institution of Chemical Engineers (IChemE)Universidade do MinhoSimões, M.Simões, Lúcia C.Machado, IdalinaPereira, Maria OlíviaVieira, Maria João20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/6061eng"Food and bioproducts processing". ISSN 0960-3085. 84:C4 (2006) 338-345.0960-308510.1205/fbp06022info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:36:12Zoai:repositorium.sdum.uminho.pt:1822/6061Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:32:13.595044Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Control of flow-generated biofilms with surfactants : evidence of resistance and recovery |
title |
Control of flow-generated biofilms with surfactants : evidence of resistance and recovery |
spellingShingle |
Control of flow-generated biofilms with surfactants : evidence of resistance and recovery Simões, M. Biofilm control Hydrodynamic conditions Recovery Resistance Surfactant Science & Technology |
title_short |
Control of flow-generated biofilms with surfactants : evidence of resistance and recovery |
title_full |
Control of flow-generated biofilms with surfactants : evidence of resistance and recovery |
title_fullStr |
Control of flow-generated biofilms with surfactants : evidence of resistance and recovery |
title_full_unstemmed |
Control of flow-generated biofilms with surfactants : evidence of resistance and recovery |
title_sort |
Control of flow-generated biofilms with surfactants : evidence of resistance and recovery |
author |
Simões, M. |
author_facet |
Simões, M. Simões, Lúcia C. Machado, Idalina Pereira, Maria Olívia Vieira, Maria João |
author_role |
author |
author2 |
Simões, Lúcia C. Machado, Idalina Pereira, Maria Olívia Vieira, Maria João |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Simões, M. Simões, Lúcia C. Machado, Idalina Pereira, Maria Olívia Vieira, Maria João |
dc.subject.por.fl_str_mv |
Biofilm control Hydrodynamic conditions Recovery Resistance Surfactant Science & Technology |
topic |
Biofilm control Hydrodynamic conditions Recovery Resistance Surfactant Science & Technology |
description |
The action of cetyltrimethyl ammonium bromide (CTAB) and sodium dodecyl sulphate (SDS), a cationic and an anionic surfactant respectively, were investigated for their ability to control turbulent and laminar flow-generated biofilms formed by P. fluorescens. The disinfectant action of CTAB and SDS on biofilms was assessed by respiratory activity, variation of mass and structure, immediately, 3, 7 and 12 h after the application of the surfactants. Laminar flow-generated biofilms were more susceptible to the action of CTAB than those formed under turbulent flow. Total inactivation of the cells within the biofilms was not achieved for either type of flow- enerated biofilms. For SDS, higher concentrations promoted significant biofilm inactivation, for both turbulent and laminar flow-generated biofilms. CTAB and SDS application did not promote the detachment of biofilms from the surfaces. Post-surfactant treatment, biofilms recovered respiratory activity, in some cases, reaching values higher than those found without chemical treatment. After CTAB treatment, the recovery of respiratory activity was not affected by the hydrodynamic conditions. Conversely, turbulent flow-generated biofilms showed a higher potential to recover their metabolic activity than laminar flow-generated biofilms, when previously challenged with SDS. Concerning biofilm mass, no significant variation (increase or decrease) was detected after 12 h of surfactant treatment. This study shows that care is needed when selecting the correct procedure and agent for biofilm control and demonstrates the influence of hydrodynamic conditions on the persistent and recalcitrant properties of P. fluorescens biofilms. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2006-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/6061 |
url |
http://hdl.handle.net/1822/6061 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Food and bioproducts processing". ISSN 0960-3085. 84:C4 (2006) 338-345. 0960-3085 10.1205/fbp06022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institution of Chemical Engineers (IChemE) |
publisher.none.fl_str_mv |
Institution of Chemical Engineers (IChemE) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132833722138624 |