Improvement of butyrylcholinesterase enzymeinhibition and medicinal properties of extracts ofAristotelia serrata leaves by ultrasound extraction

• Autor(a) principal: Majid, Hafiz
• Data de Publicação: 2020
• Outros Autores: Silva, Filipa V.M.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.5/22015
• topic: plant extracts; ultrasound extraction; optimization; yield; antioxidant activity; Alzheimer’s Disease

Aristotelia serrata, wineberry, or makomako, is a small deciduous fast-growing shrub of New Zealand that produces a berry. In this study leaf extracts were produced using ultra-sound technology. Alzheimer’s Disease is a progressive mental deterioration related with ageing and senility, in which approved drugs for treatment inhibit butyrylcholinesterase(BChE), an enzyme present in the human brain. The ultrasound assisted extraction of natural anti-butyrylcholinesterase and antioxidants from this plant, and the yield of extraction were optimized using response surface methodology. The effect of acoustic power density(0.13–0.65 W/mL), time (1–20 min), and ethanol concentration (20–80%) on BChE inhibition,antioxidant activity, and extraction yield was determined, the optimum extraction conditions being 0.53 W/mL, 17.12 min and 74% ethanol. The optimized extracts were produced to experimentally validate the predicted responses. The IC50 concentrations were experimentally determined (26.2 ug/mL for BChE, 5.5 ug/mL for radical scavenging activity). A. serrata leaf extracts have potential to be used as complements to medicines traditionally used for memory dysfunction