Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] cluster

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Pedro M.
Data de Publicação: 2006
Outros Autores: Macedo, Anjos L., Moura, Isabel, Moura, José J. G., Goodfellow, Brian J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8710
Resumo: J Biol Inorg Chem (2006) 11: 307–315 DOI 10.1007/s00775-005-0077-2
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spelling Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] clusterJ Biol Inorg Chem (2006) 11: 307–315 DOI 10.1007/s00775-005-0077-2Desulfovibrio gigas ferredoxin II (DgFdII) is a small protein with a polypeptide chain composed of 58 amino acids, containing one Fe3S4 cluster per monomer. Upon studying the redox cycle of this protein, we detected a stable intermediate (FdIIint) with four 1H resonances at 24.1, 20.5, 20.8 and 13.7 ppm. The differences between FdIIox and FdIIint were attributed to conformational changes resulting from the breaking/formation of an internal disulfide bridge. The same 1H NMR methodology used to fully assign the three cysteinyl ligands of the [3Fe-4S] core in the oxidized state (DgFdIIox) was used here for the assignment of the same three ligands in the intermediate state (DgFdIIint). The spin-coupling model used for the oxidized form of DgFdII where magnetic exchange coupling constants of around 300 cm-1 and hyperfine coupling constants equal to 1 MHz for all the three iron centres were found, does not explain the isotropic shift temperature dependence for the three cysteinyl cluster ligands in DgFdIIint. This study, together with the spin delocalization mechanism proposed here for DgFdIIint, allows the detection of structural modifications at the [3Fe-4S] cluster in DgFdIIox and DgFdIIint.SpringerRUNRodrigues, Pedro M.Macedo, Anjos L.Moura, IsabelMoura, José J. G.Goodfellow, Brian J.2013-02-06T11:45:46Z20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8710eng0949-8257info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:36Zoai:run.unl.pt:10362/8710Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:23.513819Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] cluster
title Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] cluster
spellingShingle Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] cluster
Rodrigues, Pedro M.
title_short Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] cluster
title_full Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] cluster
title_fullStr Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] cluster
title_full_unstemmed Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] cluster
title_sort Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] cluster
author Rodrigues, Pedro M.
author_facet Rodrigues, Pedro M.
Macedo, Anjos L.
Moura, Isabel
Moura, José J. G.
Goodfellow, Brian J.
author_role author
author2 Macedo, Anjos L.
Moura, Isabel
Moura, José J. G.
Goodfellow, Brian J.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Rodrigues, Pedro M.
Macedo, Anjos L.
Moura, Isabel
Moura, José J. G.
Goodfellow, Brian J.
description J Biol Inorg Chem (2006) 11: 307–315 DOI 10.1007/s00775-005-0077-2
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
2013-02-06T11:45:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8710
url http://hdl.handle.net/10362/8710
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0949-8257
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
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