Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/13012 |
Resumo: | Dissertação para a obtenção do grau de Mestre em Genética Molecular e Biomedicina |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleenNotch signaling – Delta1OrganogenesisThymusSpleenHematopoietic progenitorsB cellsDissertação para a obtenção do grau de Mestre em Genética Molecular e BiomedicinaThe lymphoid organs, thymus and spleen, are essential for the differentiation of T and B cells, respectively. One of the signaling pathways involved in processes of cell differentiation is the Notch signaling pathway. Work conducted by our group demonstrated that Notch ligands, receptors and target genes are expressed in the thymic epithelium. In this work, we aimed: 1) to study the organogenesis of the thymus and spleen in chicken embryos; 2) phenotypically characterize the cells present in both organs; 3) to study the role of Notch signaling in the differentiation of hematopoietic progenitors (PH) isolated from thymus and spleen of the chicken embryo. To study the formation of the thymus and spleen and characterize the cell populations present within them during embryonic development, we stained histological sections of the organs and citospins with Hematoxylin-Eosin (HE). To elucidate the role of Notch signaling in the differentiation of the PH, we used a co-culture system of PH with stromal cells that express specific Notch ligands, Delta 1 (Dl1) and Delta 4 (Dl4). The results confirm that, the spleen is an early (E6) vascularized organ and the number of PH in the spleen increases after day E10. In the thymus, the regionalization in cortex and medulla occurs at day E8 and there are no visible neural crest cells during the several embryonic stages studied. We conclude that hematopoietic cells collected from the thymus at day E15 are more differentiated than cells collected from thymus E13. The co-cultures results demonstrated that the Notch ligand Dl1 appears to favor the lymphoid progenitor cells (LPC) proliferation/survival. Thus, the Dl1 ligand may have an important role at the early stages of T cell differentiation. Moreover, these evidences seem to confirm that the undifferentiated LPCs are able to respond more effectively to external stimulus and differentiate into T cells more efficiently.Faculdade de Ciências e TecnologiaHenriques, IsabelZilhão, RitaRUNMoreira, Pedro Miguel Lopes2014-07-29T10:27:56Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/13012enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:47:39Zoai:run.unl.pt:10362/13012Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:21:01.160071Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen |
title |
Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen |
spellingShingle |
Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen Moreira, Pedro Miguel Lopes Notch signaling – Delta1 Organogenesis Thymus Spleen Hematopoietic progenitors B cells |
title_short |
Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen |
title_full |
Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen |
title_fullStr |
Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen |
title_full_unstemmed |
Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen |
title_sort |
Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen |
author |
Moreira, Pedro Miguel Lopes |
author_facet |
Moreira, Pedro Miguel Lopes |
author_role |
author |
dc.contributor.none.fl_str_mv |
Henriques, Isabel Zilhão, Rita RUN |
dc.contributor.author.fl_str_mv |
Moreira, Pedro Miguel Lopes |
dc.subject.por.fl_str_mv |
Notch signaling – Delta1 Organogenesis Thymus Spleen Hematopoietic progenitors B cells |
topic |
Notch signaling – Delta1 Organogenesis Thymus Spleen Hematopoietic progenitors B cells |
description |
Dissertação para a obtenção do grau de Mestre em Genética Molecular e Biomedicina |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-07-29T10:27:56Z 2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/13012 |
url |
http://hdl.handle.net/10362/13012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Faculdade de Ciências e Tecnologia |
publisher.none.fl_str_mv |
Faculdade de Ciências e Tecnologia |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137851049246720 |