High seroprevalence of Leishmania infantum is linked to immune activation in people with HIV
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/164497 |
Resumo: | Funding Information: This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001, RK; Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB, grant APP0032/2016, RK); Brazilian National Council for Scientific and Technological Development (CNPq, grant 65083/2015-8, LS); FAPESB/CNPq (008/2014 PRONEX grant 8111/2014, AB); Fonds voor Wetenschappelijk Onderzoek Vlaanderen (grant G0D6817N, A-MV and JW). None of the funding organizations had any role in the study design, data collection, data interpretation or writing of this report. Publisher Copyright: Copyright © 2023 de Moraes, Santos, Arruda, Silva, Silva, Silva, Ramos, Santos, Torres, Orge, Teixeira, Vieira, Ramírez, Soto, Grassi, Siqueira, Costa, Costa, Andrade, Akrami, de Oliveira, Boaventura, Barral-Netto, Barral, Vandamme, Van Weyenbergh and Khouri. |
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High seroprevalence of Leishmania infantum is linked to immune activation in people with HIVa two-stage cross-sectional study in Bahia, BrazilHIV-1hostpathogen interactionimmune activationLeishmania infantumvisceral leishmaniasisQR180 ImmunologyRA0421 Public health. Hygiene. Preventive MedicineMicrobiologyMicrobiology (medical)ParasitologyVirologyImmunologyApplied Microbiology and BiotechnologySDG 3 - Good Health and Well-beingSDG 9 - Industry, Innovation, and InfrastructureSDG 10 - Reduced InequalitiesFunding Information: This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001, RK; Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB, grant APP0032/2016, RK); Brazilian National Council for Scientific and Technological Development (CNPq, grant 65083/2015-8, LS); FAPESB/CNPq (008/2014 PRONEX grant 8111/2014, AB); Fonds voor Wetenschappelijk Onderzoek Vlaanderen (grant G0D6817N, A-MV and JW). None of the funding organizations had any role in the study design, data collection, data interpretation or writing of this report. Publisher Copyright: Copyright © 2023 de Moraes, Santos, Arruda, Silva, Silva, Silva, Ramos, Santos, Torres, Orge, Teixeira, Vieira, Ramírez, Soto, Grassi, Siqueira, Costa, Costa, Andrade, Akrami, de Oliveira, Boaventura, Barral-Netto, Barral, Vandamme, Van Weyenbergh and Khouri.Visceral leishmaniasis is an opportunistic disease in HIV-1 infected individuals, unrecognized as a determining factor for AIDS diagnosis. The growing geographical overlap of HIV-1 and Leishmania infections is an emerging challenge worldwide, as co-infection increases morbidity and mortality for both infections. Here, we determined the prevalence of people living with HIV (PWH) with a previous or ongoing infection by Leishmania infantum and investigated the virological and immunological factors associated with co-infection. We adopted a two-stage cross-sectional cohort (CSC) design (CSC-I, n = 5,346 and CSC-II, n = 317) of treatment-naïve HIV-1-infected individuals in Bahia, Brazil. In CSC-I, samples collected between 1998 and 2013 were used for serological screening for leishmaniasis by an in-house Enzyme-Linked Immunosorbent Assay (ELISA) with SLA (Soluble Leishmania infantum Antigen), resulting in a prevalence of previous or ongoing infection of 16.27%. Next, 317 PWH were prospectively recruited from July 2014 to December 2015 with the collection of sociodemographic and clinical data. Serological validation by two different immunoassays confirmed a prevalence of 15.46 and 8.20% by anti-SLA, and anti-HSP70 serology, respectively, whereas 4.73% were double-positive (DP). Stratification of these 317 individuals in DP and double-negative (DN) revealed a significant reduction of CD4+ counts and CD4+/CD8+ ratios and a tendency of increased viral load in the DP group, as compared to DN. No statistical differences in HIV-1 subtype distribution were observed between the two groups. However, we found a significant increase of CXCL10 (p = 0.0076) and a tendency of increased CXCL9 (p = 0.061) in individuals with DP serology, demonstrating intensified immune activation in this group. These findings were corroborated at the transcriptome level in independent Leishmania- and HIV-1-infected cohorts (Swiss HIV Cohort and Piaui Northeast Brazil Cohort), indicating that CXCL10 transcripts are shared by the IFN-dominated immune activation gene signatures of both pathogens and positively correlated to viral load in untreated PWH. This study demonstrated a high prevalence of PWH with L. infantum seropositivity in Bahia, Brazil, linked to IFN-mediated immune activation and a significant decrease in CD4+ levels. Our results highlight the urgent need to increase awareness and define public health strategies for the management and prevention of HIV-1 and L. infantum co-infection.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)TB, HIV and opportunistic diseases and pathogens (THOP)RUNde Moraes, LaiseSantos, Luciane AmorimArruda, Liã BárbaraSilva, Maria da Purificação Pereira daSilva, Márcio de OliveiraSilva, José Adriano GóesRamos, AndréSantos, Marcos Bastos dosTorres, Felipe GuimarãesOrge, CibeleTeixeira, Antonio Marcos dos SantosVieira, Thiago SantosRamírez, LauraSoto, ManuelGrassi, Maria Fernanda RiosSiqueira, Isadora Cristina deCosta, Dorcas LamounierCosta, Carlos Henrique NeryAndrade, Bruno de BezerrilAkrami, Kevande Oliveira, Camila IndianiBoaventura, Viviane SampaioBarral-Netto, ManoelBarral, AldinaVandamme, Anne MiekeVan Weyenbergh, JohanKhouri, Ricardo2024-03-05T23:49:28Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/164497eng1664-302XPURE: 83835717https://doi.org/10.3389/fmicb.2023.1221682info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:52:40Zoai:run.unl.pt:10362/164497Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:12.556185Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
High seroprevalence of Leishmania infantum is linked to immune activation in people with HIV a two-stage cross-sectional study in Bahia, Brazil |
| title |
High seroprevalence of Leishmania infantum is linked to immune activation in people with HIV |
| spellingShingle |
High seroprevalence of Leishmania infantum is linked to immune activation in people with HIV de Moraes, Laise HIV-1 hostpathogen interaction immune activation Leishmania infantum visceral leishmaniasis QR180 Immunology RA0421 Public health. Hygiene. Preventive Medicine Microbiology Microbiology (medical) Parasitology Virology Immunology Applied Microbiology and Biotechnology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 10 - Reduced Inequalities |
| title_short |
High seroprevalence of Leishmania infantum is linked to immune activation in people with HIV |
| title_full |
High seroprevalence of Leishmania infantum is linked to immune activation in people with HIV |
| title_fullStr |
High seroprevalence of Leishmania infantum is linked to immune activation in people with HIV |
| title_full_unstemmed |
High seroprevalence of Leishmania infantum is linked to immune activation in people with HIV |
| title_sort |
High seroprevalence of Leishmania infantum is linked to immune activation in people with HIV |
| author |
de Moraes, Laise |
| author_facet |
de Moraes, Laise Santos, Luciane Amorim Arruda, Liã Bárbara Silva, Maria da Purificação Pereira da Silva, Márcio de Oliveira Silva, José Adriano Góes Ramos, André Santos, Marcos Bastos dos Torres, Felipe Guimarães Orge, Cibele Teixeira, Antonio Marcos dos Santos Vieira, Thiago Santos Ramírez, Laura Soto, Manuel Grassi, Maria Fernanda Rios Siqueira, Isadora Cristina de Costa, Dorcas Lamounier Costa, Carlos Henrique Nery Andrade, Bruno de Bezerril Akrami, Kevan de Oliveira, Camila Indiani Boaventura, Viviane Sampaio Barral-Netto, Manoel Barral, Aldina Vandamme, Anne Mieke Van Weyenbergh, Johan Khouri, Ricardo |
| author_role |
author |
| author2 |
Santos, Luciane Amorim Arruda, Liã Bárbara Silva, Maria da Purificação Pereira da Silva, Márcio de Oliveira Silva, José Adriano Góes Ramos, André Santos, Marcos Bastos dos Torres, Felipe Guimarães Orge, Cibele Teixeira, Antonio Marcos dos Santos Vieira, Thiago Santos Ramírez, Laura Soto, Manuel Grassi, Maria Fernanda Rios Siqueira, Isadora Cristina de Costa, Dorcas Lamounier Costa, Carlos Henrique Nery Andrade, Bruno de Bezerril Akrami, Kevan de Oliveira, Camila Indiani Boaventura, Viviane Sampaio Barral-Netto, Manoel Barral, Aldina Vandamme, Anne Mieke Van Weyenbergh, Johan Khouri, Ricardo |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) TB, HIV and opportunistic diseases and pathogens (THOP) RUN |
| dc.contributor.author.fl_str_mv |
de Moraes, Laise Santos, Luciane Amorim Arruda, Liã Bárbara Silva, Maria da Purificação Pereira da Silva, Márcio de Oliveira Silva, José Adriano Góes Ramos, André Santos, Marcos Bastos dos Torres, Felipe Guimarães Orge, Cibele Teixeira, Antonio Marcos dos Santos Vieira, Thiago Santos Ramírez, Laura Soto, Manuel Grassi, Maria Fernanda Rios Siqueira, Isadora Cristina de Costa, Dorcas Lamounier Costa, Carlos Henrique Nery Andrade, Bruno de Bezerril Akrami, Kevan de Oliveira, Camila Indiani Boaventura, Viviane Sampaio Barral-Netto, Manoel Barral, Aldina Vandamme, Anne Mieke Van Weyenbergh, Johan Khouri, Ricardo |
| dc.subject.por.fl_str_mv |
HIV-1 hostpathogen interaction immune activation Leishmania infantum visceral leishmaniasis QR180 Immunology RA0421 Public health. Hygiene. Preventive Medicine Microbiology Microbiology (medical) Parasitology Virology Immunology Applied Microbiology and Biotechnology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 10 - Reduced Inequalities |
| topic |
HIV-1 hostpathogen interaction immune activation Leishmania infantum visceral leishmaniasis QR180 Immunology RA0421 Public health. Hygiene. Preventive Medicine Microbiology Microbiology (medical) Parasitology Virology Immunology Applied Microbiology and Biotechnology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 10 - Reduced Inequalities |
| description |
Funding Information: This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001, RK; Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB, grant APP0032/2016, RK); Brazilian National Council for Scientific and Technological Development (CNPq, grant 65083/2015-8, LS); FAPESB/CNPq (008/2014 PRONEX grant 8111/2014, AB); Fonds voor Wetenschappelijk Onderzoek Vlaanderen (grant G0D6817N, A-MV and JW). None of the funding organizations had any role in the study design, data collection, data interpretation or writing of this report. Publisher Copyright: Copyright © 2023 de Moraes, Santos, Arruda, Silva, Silva, Silva, Ramos, Santos, Torres, Orge, Teixeira, Vieira, Ramírez, Soto, Grassi, Siqueira, Costa, Costa, Andrade, Akrami, de Oliveira, Boaventura, Barral-Netto, Barral, Vandamme, Van Weyenbergh and Khouri. |
| publishDate |
2023 |
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2023 2023-01-01T00:00:00Z 2024-03-05T23:49:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/164497 |
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http://hdl.handle.net/10362/164497 |
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eng |
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eng |
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1664-302X PURE: 83835717 https://doi.org/10.3389/fmicb.2023.1221682 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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