Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals

Detalhes bibliográficos
Autor(a) principal: Silva, Vanessa
Data de Publicação: 2022
Outros Autores: Correia, Elisete, Pereira, José Eduardo, González-Machado, Camino, Capita, Rosa, Alonso-Calleja, Carlos, Igrejas, Gilberto, Poeta, Patrícia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/144511
Resumo: Funding: NORTE-01-0145-FEDER-030101 the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). The Ministerio de Ciencia, Innovación y Universidades (Spain, grant number RTI2018-098267-R-C33) and the Junta de Castilla y León (Consejería de Educación, Spain, grant number LE018P20). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild AnimalsRelationship with Clonal Lineages and Antimicrobial Resistanceanimalsantimicrobial resistancebiofilmsgenetic linagesMRSAS. aureusMicrobiologyBiochemistryPharmacology, Toxicology and Pharmaceutics(all)Microbiology (medical)Infectious DiseasesPharmacology (medical)SDG 3 - Good Health and Well-beingFunding: NORTE-01-0145-FEDER-030101 the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). The Ministerio de Ciencia, Innovación y Universidades (Spain, grant number RTI2018-098267-R-C33) and the Junta de Castilla y León (Consejería de Educación, Spain, grant number LE018P20). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.This study aimed to compare the biofilm formation ability of Staphylococcus aureus isolated from a wide range of animals and study the association between biofilm formation and antimicrobial resistance and genetic lineages. A total of 214 S. aureus strains isolated from pets, livestock, and wild animals were evaluated regarding their ability to form biofilms by the microtiter biofilm assay and their structure via confocal scanning laser microscopy. Statistical analysis was used to find an association between biofilm formation and antimicrobial resistance, multidrug resistance, sequence types (STs), spa and agr-types of the isolates. The antimicrobial susceptibility of 24 h-old biofilms was assessed against minimum inhibitory concentrations (MIC) and 10× MIC of amikacin and tetracycline, and the biomass reduction was measured. The metabolic activity of biofilms after antimicrobial treatment was evaluated by the XTT assay. All isolates were had the ability to form biofilms. Yet, significant differences in biofilm biomass production were detected among animal species. Multidrug resistance had a positive association with biofilm formation as well as methicillin-resistance. Significant differences were also detected among the clonal lineages of the isolates. Both tetracycline and amikacin were able to significantly reduce the biofilm mass. However, none of the antimicrobials were able to eradicate the biofilm at the maximum concentration used. Our results provide important information on the biofilm-forming capacity of animal-adapted S. aureus isolates, which may have potential implications for the development of new biofilm-targeted therapeutics.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNSilva, VanessaCorreia, ElisetePereira, José EduardoGonzález-Machado, CaminoCapita, RosaAlonso-Calleja, CarlosIgrejas, GilbertoPoeta, Patrícia2022-10-06T22:16:59Z2022-06-042022-06-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://hdl.handle.net/10362/144511eng2079-6382PURE: 46302471https://doi.org/10.3390/antibiotics11060772info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:24:10Zoai:run.unl.pt:10362/144511Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:36.045425Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals
Relationship with Clonal Lineages and Antimicrobial Resistance
title Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals
spellingShingle Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals
Silva, Vanessa
animals
antimicrobial resistance
biofilms
genetic linages
MRSA
S. aureus
Microbiology
Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)
SDG 3 - Good Health and Well-being
title_short Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals
title_full Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals
title_fullStr Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals
title_full_unstemmed Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals
title_sort Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals
author Silva, Vanessa
author_facet Silva, Vanessa
Correia, Elisete
Pereira, José Eduardo
González-Machado, Camino
Capita, Rosa
Alonso-Calleja, Carlos
Igrejas, Gilberto
Poeta, Patrícia
author_role author
author2 Correia, Elisete
Pereira, José Eduardo
González-Machado, Camino
Capita, Rosa
Alonso-Calleja, Carlos
Igrejas, Gilberto
Poeta, Patrícia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Silva, Vanessa
Correia, Elisete
Pereira, José Eduardo
González-Machado, Camino
Capita, Rosa
Alonso-Calleja, Carlos
Igrejas, Gilberto
Poeta, Patrícia
dc.subject.por.fl_str_mv animals
antimicrobial resistance
biofilms
genetic linages
MRSA
S. aureus
Microbiology
Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)
SDG 3 - Good Health and Well-being
topic animals
antimicrobial resistance
biofilms
genetic linages
MRSA
S. aureus
Microbiology
Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)
SDG 3 - Good Health and Well-being
description Funding: NORTE-01-0145-FEDER-030101 the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). The Ministerio de Ciencia, Innovación y Universidades (Spain, grant number RTI2018-098267-R-C33) and the Junta de Castilla y León (Consejería de Educación, Spain, grant number LE018P20). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-06T22:16:59Z
2022-06-04
2022-06-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/144511
url http://hdl.handle.net/10362/144511
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2079-6382
PURE: 46302471
https://doi.org/10.3390/antibiotics11060772
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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