Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/52554 |
Resumo: | © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited |
| id |
RCAP_e2660302ee83500eeeadae619cc1e341 |
|---|---|
| oai_identifier_str |
oai:repositorio.ul.pt:10451/52554 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax pereziPelophylax pereziAmphibiaAntioxidantBioactive peptideNeuroprotectionTryptophyllin© 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly citedTryptophyllins constitute a heterogeneous group of peptides that are one of the first classes of peptides identified from amphibian's skin secretions. Here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, isolated from the Iberian green frog Pelophylax perezi. The skin secretion of P. perezi was obtained by electrical stimulation and fractionated using RP-HPLC. De novo peptide sequencing was conducted using MALDI MS/MS. The primary structure of PpT-2 (FPWLLS-NH2 ) was confirmed by Edman degradation and subsequently investigated using in silico tools. PpT-2 shared physicochemical properties with other well-known antioxidants. To test PpT-2 for antioxidant activity in vitro, the peptide was synthesized by solid phase and assessed in the chemical-based ABTS and DPPH scavenging assays. Then, a flow cytometry experiment was conducted to assess PpT-2 antioxidant activity in oxidatively challenged murine microglial cells. As predicted by the in silico analyses, PpT-2 scavenged free radicals in vitro and suppressed the generation of reactive species in PMA-stimulated BV-2 microglia cells. We further explored possible bioactivities of PpT-2 against prostate cancer cells and bacteria, against which the peptide exerted a moderate antiproliferative effect and negligible antimicrobial activity. The biocompatibility of PpT-2 was evaluated in cytotoxicity assays and in vivo toxicity with Galleria mellonella. No toxicity was detected in cells treated with up to 512 µg/ml and in G. mellonella treated with up to 40 mg/kg PpT-2. This novel peptide, PpT-2, stands as a promising peptide with potential therapeutic and biotechnological applications, mainly for the treatment/prevention of neurodegenerative disorders.This work was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalization (POCI), and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia in the framework of the project POCI-01-0145-FEDER-031158 – PTDC/BII-BIO/31158/2017. The authors would like to thank the participation and scientific support of the Unit projects UIDB/50006/2020 | UIDP/50006/2020, and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Universal Faixa ‘B’ (grant number 32103/2018-0). A.P. is a recipient of a post-doctoral grant from the project PTDC/BII-BIO/31158/2017. The authors would like to thank the researcher Roberto Resendes (CiBio, University of the Azores, Ponta Delgada, São Miguel, Azores, Portugal) for the logistical support in the collection of samples. C.P.A acknowledges FCT-MCTES fellowship PD/BD/136860/2018. A.B.-N. and F.C.D.A.L. acknowledge CNPq (grants 420449/2018-3 and 428211/2018-6) for financial support.WileyRepositório da Universidade de LisboaPlácido, AlexandraAmaral, Constança Pais DoTeixeira, CátiaNogueira, ArianeBrango‐Vanegas, JoséAlves Barbosa, EderC. Moreira, DanielSilva‐Carvalho, Amandda E.da Silva, Maria da Gloriado Nascimento Dias, JhonesAlbuquerque, PatríciaSaldanha‐Araújo, FelipeC. D. A. Lima, FilipeBatagin‐Neto, AugustoKuckelhaus, SelmaBessa, Lucinda J.Freitas, JaimeDotto Brand, GuilhermeSantos, Nuno C.B. Relvas, JoãoGomes, PaulaS. A. Leite, José RobertoEaton, Peter2022-04-26T16:25:14Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/52554engJ Cell Mol Med. 2022 Apr 231582-183810.1111/jcmm.172921582-4934info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:57:48Zoai:repositorio.ul.pt:10451/52554Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:03:37.958246Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi |
| title |
Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi |
| spellingShingle |
Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi Plácido, Alexandra Pelophylax perezi Amphibia Antioxidant Bioactive peptide Neuroprotection Tryptophyllin |
| title_short |
Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi |
| title_full |
Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi |
| title_fullStr |
Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi |
| title_full_unstemmed |
Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi |
| title_sort |
Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi |
| author |
Plácido, Alexandra |
| author_facet |
Plácido, Alexandra Amaral, Constança Pais Do Teixeira, Cátia Nogueira, Ariane Brango‐Vanegas, José Alves Barbosa, Eder C. Moreira, Daniel Silva‐Carvalho, Amandda E. da Silva, Maria da Gloria do Nascimento Dias, Jhones Albuquerque, Patrícia Saldanha‐Araújo, Felipe C. D. A. Lima, Filipe Batagin‐Neto, Augusto Kuckelhaus, Selma Bessa, Lucinda J. Freitas, Jaime Dotto Brand, Guilherme Santos, Nuno C. B. Relvas, João Gomes, Paula S. A. Leite, José Roberto Eaton, Peter |
| author_role |
author |
| author2 |
Amaral, Constança Pais Do Teixeira, Cátia Nogueira, Ariane Brango‐Vanegas, José Alves Barbosa, Eder C. Moreira, Daniel Silva‐Carvalho, Amandda E. da Silva, Maria da Gloria do Nascimento Dias, Jhones Albuquerque, Patrícia Saldanha‐Araújo, Felipe C. D. A. Lima, Filipe Batagin‐Neto, Augusto Kuckelhaus, Selma Bessa, Lucinda J. Freitas, Jaime Dotto Brand, Guilherme Santos, Nuno C. B. Relvas, João Gomes, Paula S. A. Leite, José Roberto Eaton, Peter |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Plácido, Alexandra Amaral, Constança Pais Do Teixeira, Cátia Nogueira, Ariane Brango‐Vanegas, José Alves Barbosa, Eder C. Moreira, Daniel Silva‐Carvalho, Amandda E. da Silva, Maria da Gloria do Nascimento Dias, Jhones Albuquerque, Patrícia Saldanha‐Araújo, Felipe C. D. A. Lima, Filipe Batagin‐Neto, Augusto Kuckelhaus, Selma Bessa, Lucinda J. Freitas, Jaime Dotto Brand, Guilherme Santos, Nuno C. B. Relvas, João Gomes, Paula S. A. Leite, José Roberto Eaton, Peter |
| dc.subject.por.fl_str_mv |
Pelophylax perezi Amphibia Antioxidant Bioactive peptide Neuroprotection Tryptophyllin |
| topic |
Pelophylax perezi Amphibia Antioxidant Bioactive peptide Neuroprotection Tryptophyllin |
| description |
© 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-04-26T16:25:14Z 2022 2022-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/52554 |
| url |
http://hdl.handle.net/10451/52554 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
J Cell Mol Med. 2022 Apr 23 1582-1838 10.1111/jcmm.17292 1582-4934 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley |
| publisher.none.fl_str_mv |
Wiley |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799134586973716480 |