Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples

Detalhes bibliográficos
Autor(a) principal: Pina-Vaz, Cidália
Data de Publicação: 2004
Outros Autores: Costa-de-Oliveira, Sofia, Rodrigues, Acácio G., Salvador, Alexandre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/2457
Resumo: In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.
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spelling Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samplesTuberculosisMycobacteriaBacilliBacteriaAuraminePropidium iodideCytometryIn order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.American Society for MicrobiologyRCIPLPina-Vaz, CidáliaCosta-de-Oliveira, SofiaRodrigues, Acácio G.Salvador, Alexandre2013-04-23T14:10:53Z2004-022004-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/2457engPina-Vaz C, Costa-de-Oliveira S, Rodrigues AG, Salvador A. Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples. J Clin Microbiol. 2004;42(2):906-8.0095-1137info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:41:39Zoai:repositorio.ipl.pt:10400.21/2457Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:12:14.424324Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples
title Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples
spellingShingle Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples
Pina-Vaz, Cidália
Tuberculosis
Mycobacteria
Bacilli
Bacteria
Auramine
Propidium iodide
Cytometry
title_short Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples
title_full Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples
title_fullStr Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples
title_full_unstemmed Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples
title_sort Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples
author Pina-Vaz, Cidália
author_facet Pina-Vaz, Cidália
Costa-de-Oliveira, Sofia
Rodrigues, Acácio G.
Salvador, Alexandre
author_role author
author2 Costa-de-Oliveira, Sofia
Rodrigues, Acácio G.
Salvador, Alexandre
author2_role author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Pina-Vaz, Cidália
Costa-de-Oliveira, Sofia
Rodrigues, Acácio G.
Salvador, Alexandre
dc.subject.por.fl_str_mv Tuberculosis
Mycobacteria
Bacilli
Bacteria
Auramine
Propidium iodide
Cytometry
topic Tuberculosis
Mycobacteria
Bacilli
Bacteria
Auramine
Propidium iodide
Cytometry
description In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.
publishDate 2004
dc.date.none.fl_str_mv 2004-02
2004-02-01T00:00:00Z
2013-04-23T14:10:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/2457
url http://hdl.handle.net/10400.21/2457
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pina-Vaz C, Costa-de-Oliveira S, Rodrigues AG, Salvador A. Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples. J Clin Microbiol. 2004;42(2):906-8.
0095-1137
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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