Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parameters

Detalhes bibliográficos
Autor(a) principal: Moreira, Helena R.
Data de Publicação: 2020
Outros Autores: Silva, Lucília P. da, Reis, R. L., Marques, Alexandra P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/64546
Resumo: The following are available online at http://www.mdpi.com/2073-4360/12/2/329/s1, Figure S1: Freezing profiles of 0.75% GG hydrogels, Figure S2: Effect of freezing conditions (standard method, NFD, and IFD at −20, −80, and −210 ◦C) over the properties of 0.75% GG dried polymeric structures and spongy-like hydrogels., Figure S3: Water uptake of 1.25% GG spongy-like hydrogels at −20, −80, and −210 ◦C using different freezing systems, Figure S4: Scaffolds reproducibility, Figure S5: Effect of freezing conditions (standard method, NFD, and IFD at −20, −80, and −210 ◦C) over hDFb behavior in 0.75% GG spongy-like hydrogels.
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spelling Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parametersspongy-like hydrogelsfreezing devicemicrostructureScience & TechnologyThe following are available online at http://www.mdpi.com/2073-4360/12/2/329/s1, Figure S1: Freezing profiles of 0.75% GG hydrogels, Figure S2: Effect of freezing conditions (standard method, NFD, and IFD at −20, −80, and −210 ◦C) over the properties of 0.75% GG dried polymeric structures and spongy-like hydrogels., Figure S3: Water uptake of 1.25% GG spongy-like hydrogels at −20, −80, and −210 ◦C using different freezing systems, Figure S4: Scaffolds reproducibility, Figure S5: Effect of freezing conditions (standard method, NFD, and IFD at −20, −80, and −210 ◦C) over hDFb behavior in 0.75% GG spongy-like hydrogels.Gellan gum (GG) spongy-like hydrogels have been explored for different tissue engineering (TE) applications owing to their highly attractive hydrogel-like features, and improved mechanical resilience and cell performance. Although the whole process for the preparation of these materials is well-defined, we hypothesized that variations occurring during the freezing step lead to batch-to-batch discrepancies. Aiming to address this issue, two freezing devices were tested, to prepare GG spongy-like hydrogels in a more reproducible way. The cooling and freezing rates, the nucleation time and temperature, and the end freezing time were determined at different freezing temperatures (−20, −80, and −210 °C). The efficacy of the devices was assessed by analyzing the physicochemical, mechanical, and biological properties of different formulations. The cooling rate and freezing rate varied between 0.1 and 128 °C/min, depending on the temperature used and the device. The properties of spongy-like hydrogels prepared with the tested devices showed lower standard deviation in comparison to those prepared with the standard process, due to the slower freezing rate of the hydrogels. However, with this method, mean pore size was significantly lower than that with the standard method. Cell entrapment, adhesion, and viability were not affected as demonstrated with human dermal fibroblasts. This work confirmed that batch-to-batch variations are mostly due to the freezing step and that the tested devices allow fine tuning of the scaffolds’ structure and properties.This research was funded by UIDB/50026/2020 Co- financiado pelo Orçamento do Estado do Ministério da Ciência, Tecnologia e Ensino Superior e, por verbas do Fundo Social Europeu através dos Programas Operacionais Regionais do Portugal 2020.The authors would like to acknowledge Daniel Rodrigues, Ângela Ferreira, and Joana Pires for the support on experimental assays, NORTE-08-5369-FSE-000037 (H.R.M.); The Discoveries Centre for Regenerative and Precision Medicine (H2020-WIDESPREAD-2014-1-739572).Multidisciplinary Digital Publishing InstituteUniversidade do MinhoMoreira, Helena R.Silva, Lucília P. daReis, R. L.Marques, Alexandra P.2020-02-052020-02-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64546engMoreira, H.R.; Silva, L.P.; Reis, R.L.; Marques, A.P. Tailoring Gellan Gum Spongy-Like Hydrogels’ Microstructure by Controlling Freezing Parameters. Polymers 2020, 12, 329.2073-436010.3390/polym12020329https://www.mdpi.com/2073-4360/12/2/329info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:29Zoai:repositorium.sdum.uminho.pt:1822/64546Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:40:57.918457Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parameters
title Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parameters
spellingShingle Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parameters
Moreira, Helena R.
spongy-like hydrogels
freezing device
microstructure
Science & Technology
title_short Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parameters
title_full Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parameters
title_fullStr Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parameters
title_full_unstemmed Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parameters
title_sort Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freezing parameters
author Moreira, Helena R.
author_facet Moreira, Helena R.
Silva, Lucília P. da
Reis, R. L.
Marques, Alexandra P.
author_role author
author2 Silva, Lucília P. da
Reis, R. L.
Marques, Alexandra P.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Moreira, Helena R.
Silva, Lucília P. da
Reis, R. L.
Marques, Alexandra P.
dc.subject.por.fl_str_mv spongy-like hydrogels
freezing device
microstructure
Science & Technology
topic spongy-like hydrogels
freezing device
microstructure
Science & Technology
description The following are available online at http://www.mdpi.com/2073-4360/12/2/329/s1, Figure S1: Freezing profiles of 0.75% GG hydrogels, Figure S2: Effect of freezing conditions (standard method, NFD, and IFD at −20, −80, and −210 ◦C) over the properties of 0.75% GG dried polymeric structures and spongy-like hydrogels., Figure S3: Water uptake of 1.25% GG spongy-like hydrogels at −20, −80, and −210 ◦C using different freezing systems, Figure S4: Scaffolds reproducibility, Figure S5: Effect of freezing conditions (standard method, NFD, and IFD at −20, −80, and −210 ◦C) over hDFb behavior in 0.75% GG spongy-like hydrogels.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-05
2020-02-05T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/64546
url http://hdl.handle.net/1822/64546
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Moreira, H.R.; Silva, L.P.; Reis, R.L.; Marques, A.P. Tailoring Gellan Gum Spongy-Like Hydrogels’ Microstructure by Controlling Freezing Parameters. Polymers 2020, 12, 329.
2073-4360
10.3390/polym12020329
https://www.mdpi.com/2073-4360/12/2/329
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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