Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/20366 |
Resumo: | A critical aspect in the development of biomaterials is the optimization of their surface properties to achieve an adequate cell response. In the present work, electrospun polycaprolactone nanofiber meshes (NFMs) are treated by radio-frequency (RF) plasma using different gases (Ar or O2), power (20 or 30 W), and exposure time (5 or 10 min). Morphological and roughness analysis show topographical changes on the plasma-treated NFMs. X-ray photoelectron spectroscopy (XPS) results indicate an increment of the oxygen-containing groups, mainly –OH and –C––O, at the plasma-treated surfaces. Accordingly, the glycerol contact angle results demonstrate a decrease in the hydrophobicity of plasma-treated meshes, particularly in the O2-treated ones. Three model cell lines (fibroblasts, chondrocytes, and osteoblasts) are used to study the effect of plasma treatments over the morphology, cell adhesion, and proliferation. A plasma treatment with O2 and one with Ar are found to be the most successful for all the studied cell types. The influence of hydrophilicity and roughness of those NFMs on their biological performance is discussed. Despite the often claimed morphological similarity of NFMs to natural extracellular matrixes, their surface properties contribute substantially to the cellular performance and therefore those should be optimized. |
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Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performancebDegradable materialsBiological activityNanofibersSurface analysisWettingbiodegradable materialsScience & TechnologyA critical aspect in the development of biomaterials is the optimization of their surface properties to achieve an adequate cell response. In the present work, electrospun polycaprolactone nanofiber meshes (NFMs) are treated by radio-frequency (RF) plasma using different gases (Ar or O2), power (20 or 30 W), and exposure time (5 or 10 min). Morphological and roughness analysis show topographical changes on the plasma-treated NFMs. X-ray photoelectron spectroscopy (XPS) results indicate an increment of the oxygen-containing groups, mainly –OH and –C––O, at the plasma-treated surfaces. Accordingly, the glycerol contact angle results demonstrate a decrease in the hydrophobicity of plasma-treated meshes, particularly in the O2-treated ones. Three model cell lines (fibroblasts, chondrocytes, and osteoblasts) are used to study the effect of plasma treatments over the morphology, cell adhesion, and proliferation. A plasma treatment with O2 and one with Ar are found to be the most successful for all the studied cell types. The influence of hydrophilicity and roughness of those NFMs on their biological performance is discussed. Despite the often claimed morphological similarity of NFMs to natural extracellular matrixes, their surface properties contribute substantially to the cellular performance and therefore those should be optimized.This work was partially supported by the European Integrated Project GENOSTEM (LSH-STREP-CT-2003-503161) and the European Network of Excellence EXPERTISSUES (NMP3-CT2004-500283). The Portuguese Foundation for Science and Technology for the project Naturally Nano (POCI/EME/589821 2004) and the Ph.D. grant of A. Martins (SFRH/BD/24382/2005) is also acknowledged.WileyUniversidade do MinhoMartins, AlbinoPinho, Elisabete D.Faria, SusanaPashkuleva, I.Marques, A. P.Reis, R. L.Neves, N. M.20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20366eng1613-682910.1002/smll.20080164819242938info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:33:02Zoai:repositorium.sdum.uminho.pt:1822/20366Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:28:28.659754Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance |
title |
Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance |
spellingShingle |
Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance Martins, Albino bDegradable materials Biological activity Nanofibers Surface analysis Wetting biodegradable materials Science & Technology |
title_short |
Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance |
title_full |
Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance |
title_fullStr |
Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance |
title_full_unstemmed |
Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance |
title_sort |
Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance |
author |
Martins, Albino |
author_facet |
Martins, Albino Pinho, Elisabete D. Faria, Susana Pashkuleva, I. Marques, A. P. Reis, R. L. Neves, N. M. |
author_role |
author |
author2 |
Pinho, Elisabete D. Faria, Susana Pashkuleva, I. Marques, A. P. Reis, R. L. Neves, N. M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Martins, Albino Pinho, Elisabete D. Faria, Susana Pashkuleva, I. Marques, A. P. Reis, R. L. Neves, N. M. |
dc.subject.por.fl_str_mv |
bDegradable materials Biological activity Nanofibers Surface analysis Wetting biodegradable materials Science & Technology |
topic |
bDegradable materials Biological activity Nanofibers Surface analysis Wetting biodegradable materials Science & Technology |
description |
A critical aspect in the development of biomaterials is the optimization of their surface properties to achieve an adequate cell response. In the present work, electrospun polycaprolactone nanofiber meshes (NFMs) are treated by radio-frequency (RF) plasma using different gases (Ar or O2), power (20 or 30 W), and exposure time (5 or 10 min). Morphological and roughness analysis show topographical changes on the plasma-treated NFMs. X-ray photoelectron spectroscopy (XPS) results indicate an increment of the oxygen-containing groups, mainly –OH and –C––O, at the plasma-treated surfaces. Accordingly, the glycerol contact angle results demonstrate a decrease in the hydrophobicity of plasma-treated meshes, particularly in the O2-treated ones. Three model cell lines (fibroblasts, chondrocytes, and osteoblasts) are used to study the effect of plasma treatments over the morphology, cell adhesion, and proliferation. A plasma treatment with O2 and one with Ar are found to be the most successful for all the studied cell types. The influence of hydrophilicity and roughness of those NFMs on their biological performance is discussed. Despite the often claimed morphological similarity of NFMs to natural extracellular matrixes, their surface properties contribute substantially to the cellular performance and therefore those should be optimized. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/20366 |
url |
http://hdl.handle.net/1822/20366 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1613-6829 10.1002/smll.200801648 19242938 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132779950112768 |