A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic Therapy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2024 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/162426 |
Resumo: | Funding Information: This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (NanoMOFdeli), ERC-2016-COG 726380, and the EPSRC (EP/S009000/1). This work was supported by the FCT PhD Scholarship (2020.06638.BD, Diana P. Sousa) and the European Research Council Starting Grant ERC-StG-2019-848325 (2019–2024, João Conde, João Conniot, and Bárbara Mendes). The Talos F200X G2 TEM was supported through an EPSRC Underpinning Multi-User Equipment Grant (EP/P030467/1). The authors thank the staff of PROXIMA-2A (Soleil Synchrotron, France) for assistance and acknowledge the beamtime under the in-house research proposal 99220022 and standard proposal 20220874. They also thank Dr. Karin Mueller and Dr. Filomena Gallo from the Cambridge Advanced Imaging Centre, Department of Physiology and Neuroscience, University of Cambridge, for assistance in performing TEM imaging on the stained cells. The authors thank Histopathology Unit at Gulbenkian Science Institute for quantitative pathology evaluation and Histological Facility at Nova Medical School for technical assistance in sample preparation. TOC and a,b were created with BioRender.com. Publisher Copyright: © 2024 The Authors. Published by American Chemical Society |
| id |
RCAP_e6e9fc170246508eb0a081e5b0956713 |
|---|---|
| oai_identifier_str |
oai:run.unl.pt:10362/162426 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic TherapyCatalysisChemistry(all)BiochemistryColloid and Surface ChemistrySDG 3 - Good Health and Well-beingFunding Information: This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (NanoMOFdeli), ERC-2016-COG 726380, and the EPSRC (EP/S009000/1). This work was supported by the FCT PhD Scholarship (2020.06638.BD, Diana P. Sousa) and the European Research Council Starting Grant ERC-StG-2019-848325 (2019–2024, João Conde, João Conniot, and Bárbara Mendes). The Talos F200X G2 TEM was supported through an EPSRC Underpinning Multi-User Equipment Grant (EP/P030467/1). The authors thank the staff of PROXIMA-2A (Soleil Synchrotron, France) for assistance and acknowledge the beamtime under the in-house research proposal 99220022 and standard proposal 20220874. They also thank Dr. Karin Mueller and Dr. Filomena Gallo from the Cambridge Advanced Imaging Centre, Department of Physiology and Neuroscience, University of Cambridge, for assistance in performing TEM imaging on the stained cells. The authors thank Histopathology Unit at Gulbenkian Science Institute for quantitative pathology evaluation and Histological Facility at Nova Medical School for technical assistance in sample preparation. TOC and a,b were created with BioRender.com. Publisher Copyright: © 2024 The Authors. Published by American Chemical SocietyPhotodynamic therapy (PDT), an emergent noninvasive cancer treatment, is largely dependent on the presence of efficient photosensitizers (PSs) and a sufficient oxygen supply. However, the therapeutic efficacy of PSs is greatly compromised by poor solubility, aggregation tendency, and oxygen depletion within solid tumors during PDT in hypoxic microenvironments. Despite the potential of PS-based metal-organic frameworks (MOFs), addressing hypoxia remains challenging. Boron dipyrromethene (BODIPY) chromophores, with excellent photostability, have exhibited great potential in PDT and bioimaging. However, their practical application suffers from limited chemical stability under harsh MOF synthesis conditions. Herein, we report the synthesis of the first example of a Zr-based MOF, namely, 69-L2, exclusively constructed from the BODIPY-derived ligands via a single-crystal to single-crystal post-synthetic exchange, where a direct solvothermal method is not applicable. To increase the PDT performance in hypoxia, we modify 69-L2 with fluorinated phosphate-functionalized methoxy poly(ethylene glycol). The resulting 69-L2@F is an oxygen carrier, enabling tumor oxygenation and simultaneously acting as a PS for reactive oxygen species (ROS) generation under LED irradiation. We demonstrate that 69-L2@F has an enhanced PDT effect in triple-negative breast cancer MDA-MB-231 cells under both normoxia and hypoxia. Following positive results, we evaluated the in vivo activity of 69-L2@F with a hydrogel, enabling local therapy in a triple-negative breast cancer mice model and achieving exceptional antitumor efficacy in only 2 days. We envision BODIPY-based Zr-MOFs to provide a solution for hypoxia relief and maximize efficacy during in vivo PDT, offering new insights into the design of promising MOF-based PSs for hypoxic tumors.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centre for Toxicogenomics and Human Health (ToxOmics)RUNChen, XuMendes, Bárbara B.Zhuang, YunhuiConniot, JoãoMercado Argandona, SergioMelle, FrancescaSousa, Diana P.Perl, DavidChivu, AlexandruPatra, Hirak K.Shepard, WilliamConde, JoãoFairen-Jimenez, David2024-01-17T22:37:33Z2024-012024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/162426eng0002-7863PURE: 81713768https://doi.org/10.1021/jacs.3c12416info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:45:21Zoai:run.unl.pt:10362/162426Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:54.292491Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic Therapy |
| title |
A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic Therapy |
| spellingShingle |
A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic Therapy Chen, Xu Catalysis Chemistry(all) Biochemistry Colloid and Surface Chemistry SDG 3 - Good Health and Well-being |
| title_short |
A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic Therapy |
| title_full |
A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic Therapy |
| title_fullStr |
A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic Therapy |
| title_full_unstemmed |
A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic Therapy |
| title_sort |
A Fluorinated BODIPY-Based Zirconium Metal-Organic Framework for In Vivo Enhanced Photodynamic Therapy |
| author |
Chen, Xu |
| author_facet |
Chen, Xu Mendes, Bárbara B. Zhuang, Yunhui Conniot, João Mercado Argandona, Sergio Melle, Francesca Sousa, Diana P. Perl, David Chivu, Alexandru Patra, Hirak K. Shepard, William Conde, João Fairen-Jimenez, David |
| author_role |
author |
| author2 |
Mendes, Bárbara B. Zhuang, Yunhui Conniot, João Mercado Argandona, Sergio Melle, Francesca Sousa, Diana P. Perl, David Chivu, Alexandru Patra, Hirak K. Shepard, William Conde, João Fairen-Jimenez, David |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centre for Toxicogenomics and Human Health (ToxOmics) RUN |
| dc.contributor.author.fl_str_mv |
Chen, Xu Mendes, Bárbara B. Zhuang, Yunhui Conniot, João Mercado Argandona, Sergio Melle, Francesca Sousa, Diana P. Perl, David Chivu, Alexandru Patra, Hirak K. Shepard, William Conde, João Fairen-Jimenez, David |
| dc.subject.por.fl_str_mv |
Catalysis Chemistry(all) Biochemistry Colloid and Surface Chemistry SDG 3 - Good Health and Well-being |
| topic |
Catalysis Chemistry(all) Biochemistry Colloid and Surface Chemistry SDG 3 - Good Health and Well-being |
| description |
Funding Information: This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (NanoMOFdeli), ERC-2016-COG 726380, and the EPSRC (EP/S009000/1). This work was supported by the FCT PhD Scholarship (2020.06638.BD, Diana P. Sousa) and the European Research Council Starting Grant ERC-StG-2019-848325 (2019–2024, João Conde, João Conniot, and Bárbara Mendes). The Talos F200X G2 TEM was supported through an EPSRC Underpinning Multi-User Equipment Grant (EP/P030467/1). The authors thank the staff of PROXIMA-2A (Soleil Synchrotron, France) for assistance and acknowledge the beamtime under the in-house research proposal 99220022 and standard proposal 20220874. They also thank Dr. Karin Mueller and Dr. Filomena Gallo from the Cambridge Advanced Imaging Centre, Department of Physiology and Neuroscience, University of Cambridge, for assistance in performing TEM imaging on the stained cells. The authors thank Histopathology Unit at Gulbenkian Science Institute for quantitative pathology evaluation and Histological Facility at Nova Medical School for technical assistance in sample preparation. TOC and a,b were created with BioRender.com. Publisher Copyright: © 2024 The Authors. Published by American Chemical Society |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-01-17T22:37:33Z 2024-01 2024-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/162426 |
| url |
http://hdl.handle.net/10362/162426 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0002-7863 PURE: 81713768 https://doi.org/10.1021/jacs.3c12416 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799138169727221760 |