Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.9/2925 |
Resumo: | Mannosylerythritol lipids (MEL) are glycolipid biosurfactants, produced by Pseudozyma spp., with increasing commercial interest. While MEL can be produced from d-glucose and d-xylose, the direct conversion of the respective lignocellulosic polysaccharides, cellulose and xylan, was not reported yet. The ability of Pseudozyma antarctica PYCC 5048T and Pseudozyma aphidis PYCC 5535T to use cellulose (Avicel®) and xylan (beechwood) as carbon and energy source has been assessed along with their capacity of producing cellulolytic and hemicellulolytic enzymes, toward a consolidated bioprocess (CBP) for MEL production. The yeasts assessed were neither able to grow in medium containing Avicel® nor produce cellulolytic enzymes under the conditions tested. On contrary, both yeasts were able to efficiently grow in xylan, but MEL production was only detected in P. antarctica PYCC 5048T cultures. MEL titers reached 1.3 g/l after 10 days in batch cultures with 40 g/l xylan, and 2.0 g/l in fed-batch cultures with xylan feeding (additional 40 g/l) at day 4. High levels of xylanase activities were detected in xylan cultures, reaching 47–62 U/ml (31–32 U/mg) at 50 °C, and still exhibiting more than 10 U/ml under physiological temperature (28 °C). Total ß-xylosidase activities, displayed mainly as wall-bounded and extracellular activity, accounted for 0.154 and 0.176 U/ml in P. antarctica PYCC 5048T and P. aphidis PYCC 5535T cultures, respectively. The present results demonstrate the potential of Pseudozyma spp. for using directly a fraction of lignocellulosic biomass, xylan, and combining in the same bioprocess the production of xylanolytic enzymes with MEL production. |
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Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048TConsolidated bioprocessingLipidsXylanCellulosePseudozyma spp.Cellulosic materialsMannosylerythritol lipids (MEL) are glycolipid biosurfactants, produced by Pseudozyma spp., with increasing commercial interest. While MEL can be produced from d-glucose and d-xylose, the direct conversion of the respective lignocellulosic polysaccharides, cellulose and xylan, was not reported yet. The ability of Pseudozyma antarctica PYCC 5048T and Pseudozyma aphidis PYCC 5535T to use cellulose (Avicel®) and xylan (beechwood) as carbon and energy source has been assessed along with their capacity of producing cellulolytic and hemicellulolytic enzymes, toward a consolidated bioprocess (CBP) for MEL production. The yeasts assessed were neither able to grow in medium containing Avicel® nor produce cellulolytic enzymes under the conditions tested. On contrary, both yeasts were able to efficiently grow in xylan, but MEL production was only detected in P. antarctica PYCC 5048T cultures. MEL titers reached 1.3 g/l after 10 days in batch cultures with 40 g/l xylan, and 2.0 g/l in fed-batch cultures with xylan feeding (additional 40 g/l) at day 4. High levels of xylanase activities were detected in xylan cultures, reaching 47–62 U/ml (31–32 U/mg) at 50 °C, and still exhibiting more than 10 U/ml under physiological temperature (28 °C). Total ß-xylosidase activities, displayed mainly as wall-bounded and extracellular activity, accounted for 0.154 and 0.176 U/ml in P. antarctica PYCC 5048T and P. aphidis PYCC 5535T cultures, respectively. The present results demonstrate the potential of Pseudozyma spp. for using directly a fraction of lignocellulosic biomass, xylan, and combining in the same bioprocess the production of xylanolytic enzymes with MEL production.ElsevierRepositório do LNEGFaria, Nuno TorresMarques, SusanaFonseca, CésarFerreira, Frederico Castelo2016-04-11T10:16:12Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/2925engFaria, N.T.; Marques, S.; Fonseca, C.; Ferreira, F.C. - Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T. In: Enzyme and Microbial Technology, 2015, Vol. 71, p. 58-650141-022910.1016/j.enzmictec.2014.10.008info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-11T04:55:17Zoai:repositorio.lneg.pt:10400.9/2925Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:35:58.030996Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
spellingShingle |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T Faria, Nuno Torres Consolidated bioprocessing Lipids Xylan Cellulose Pseudozyma spp. Cellulosic materials |
title_short |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title_full |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title_fullStr |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title_full_unstemmed |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title_sort |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
author |
Faria, Nuno Torres |
author_facet |
Faria, Nuno Torres Marques, Susana Fonseca, César Ferreira, Frederico Castelo |
author_role |
author |
author2 |
Marques, Susana Fonseca, César Ferreira, Frederico Castelo |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório do LNEG |
dc.contributor.author.fl_str_mv |
Faria, Nuno Torres Marques, Susana Fonseca, César Ferreira, Frederico Castelo |
dc.subject.por.fl_str_mv |
Consolidated bioprocessing Lipids Xylan Cellulose Pseudozyma spp. Cellulosic materials |
topic |
Consolidated bioprocessing Lipids Xylan Cellulose Pseudozyma spp. Cellulosic materials |
description |
Mannosylerythritol lipids (MEL) are glycolipid biosurfactants, produced by Pseudozyma spp., with increasing commercial interest. While MEL can be produced from d-glucose and d-xylose, the direct conversion of the respective lignocellulosic polysaccharides, cellulose and xylan, was not reported yet. The ability of Pseudozyma antarctica PYCC 5048T and Pseudozyma aphidis PYCC 5535T to use cellulose (Avicel®) and xylan (beechwood) as carbon and energy source has been assessed along with their capacity of producing cellulolytic and hemicellulolytic enzymes, toward a consolidated bioprocess (CBP) for MEL production. The yeasts assessed were neither able to grow in medium containing Avicel® nor produce cellulolytic enzymes under the conditions tested. On contrary, both yeasts were able to efficiently grow in xylan, but MEL production was only detected in P. antarctica PYCC 5048T cultures. MEL titers reached 1.3 g/l after 10 days in batch cultures with 40 g/l xylan, and 2.0 g/l in fed-batch cultures with xylan feeding (additional 40 g/l) at day 4. High levels of xylanase activities were detected in xylan cultures, reaching 47–62 U/ml (31–32 U/mg) at 50 °C, and still exhibiting more than 10 U/ml under physiological temperature (28 °C). Total ß-xylosidase activities, displayed mainly as wall-bounded and extracellular activity, accounted for 0.154 and 0.176 U/ml in P. antarctica PYCC 5048T and P. aphidis PYCC 5535T cultures, respectively. The present results demonstrate the potential of Pseudozyma spp. for using directly a fraction of lignocellulosic biomass, xylan, and combining in the same bioprocess the production of xylanolytic enzymes with MEL production. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2016-04-11T10:16:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.9/2925 |
url |
http://hdl.handle.net/10400.9/2925 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Faria, N.T.; Marques, S.; Fonseca, C.; Ferreira, F.C. - Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T. In: Enzyme and Microbial Technology, 2015, Vol. 71, p. 58-65 0141-0229 10.1016/j.enzmictec.2014.10.008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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