The first crystal structure of class III superoxide reductase from Treponema pallidum

Detalhes bibliográficos
Autor(a) principal: Santos-Silva, Teresa
Data de Publicação: 2006
Outros Autores: Trincão, José, Carvalho, Ana Luísa, Bonifácio, Cecília, Auchère, Françoise, Raleiras, Patrícia, Moura, Isabel, Moura, José J. G., Romão, Maria J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8707
Resumo: J Biol Inorg Chem (2006) 11: 548–558 DOI 10.1007/s00775-006-0104-y
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spelling The first crystal structure of class III superoxide reductase from Treponema pallidumSuperoxide reductaseTreponema pallidumOxidative stressIron–proteinSyphilisJ Biol Inorg Chem (2006) 11: 548–558 DOI 10.1007/s00775-006-0104-ySuperoxide reductase (SOR) is a metalloprotein containing a non-heme iron centre, responsible for the scavenging of superoxide radicals in the cell. The crystal structure of Treponema pallidum (Tp) SOR was determined using soft X-rays and synchrotron radiation. Crystals of the oxidized form were obtained using poly(ethylene glycol) and MgCl2 and diffracted beyond 1.55 A resolution. The overall architecture is very similar to that of other known SORs but TpSOR contains an N-terminal domain in which the desulforedoxin-type Fe centre, found in other SORs, is absent. This domain conserves the beta-barrel topology with an overall arrangement very similar to that of other SOR proteins where the centre is present. The absence of the iron ion and its ligands, however, causes a decrease in the cohesion of the domain and some disorder is observed, particularly in the region where the metal would be harboured. The C-terminal domain exhibits the characteristic immunoglobulin-like fold and harbours the Fe(His)4(Cys) active site. The five ligands of the iron centre are well conserved despite some disorder observed for one of the four molecules in the asymmetric unit. The participation of a glutamate as the sixth ligand of some of the iron centres in Pyrococcus furiosus SOR was not observed in TpSOR. A possible explanation is that either X-ray photoreduction occurred or there was a mixture of redox states at the start of data collection. In agreement with earlier proposals, details in the TpSOR structure also suggest that Lys49 might be involved in attraction of superoxide to the active site.SpringerRUNSantos-Silva, TeresaTrincão, JoséCarvalho, Ana LuísaBonifácio, CecíliaAuchère, FrançoiseRaleiras, PatríciaMoura, IsabelMoura, José J. G.Romão, Maria J.2013-02-06T11:00:54Z20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8707eng0949-8257info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:35Zoai:run.unl.pt:10362/8707Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:23.421471Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The first crystal structure of class III superoxide reductase from Treponema pallidum
title The first crystal structure of class III superoxide reductase from Treponema pallidum
spellingShingle The first crystal structure of class III superoxide reductase from Treponema pallidum
Santos-Silva, Teresa
Superoxide reductase
Treponema pallidum
Oxidative stress
Iron–protein
Syphilis
title_short The first crystal structure of class III superoxide reductase from Treponema pallidum
title_full The first crystal structure of class III superoxide reductase from Treponema pallidum
title_fullStr The first crystal structure of class III superoxide reductase from Treponema pallidum
title_full_unstemmed The first crystal structure of class III superoxide reductase from Treponema pallidum
title_sort The first crystal structure of class III superoxide reductase from Treponema pallidum
author Santos-Silva, Teresa
author_facet Santos-Silva, Teresa
Trincão, José
Carvalho, Ana Luísa
Bonifácio, Cecília
Auchère, Françoise
Raleiras, Patrícia
Moura, Isabel
Moura, José J. G.
Romão, Maria J.
author_role author
author2 Trincão, José
Carvalho, Ana Luísa
Bonifácio, Cecília
Auchère, Françoise
Raleiras, Patrícia
Moura, Isabel
Moura, José J. G.
Romão, Maria J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Santos-Silva, Teresa
Trincão, José
Carvalho, Ana Luísa
Bonifácio, Cecília
Auchère, Françoise
Raleiras, Patrícia
Moura, Isabel
Moura, José J. G.
Romão, Maria J.
dc.subject.por.fl_str_mv Superoxide reductase
Treponema pallidum
Oxidative stress
Iron–protein
Syphilis
topic Superoxide reductase
Treponema pallidum
Oxidative stress
Iron–protein
Syphilis
description J Biol Inorg Chem (2006) 11: 548–558 DOI 10.1007/s00775-006-0104-y
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
2013-02-06T11:00:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8707
url http://hdl.handle.net/10362/8707
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0949-8257
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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