Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/102840 https://doi.org/10.3390/app12073625 |
Resumo: | This paper presents a potentiometric biosensor for the detection of amyloid -42 (A -42) in point-of-care analysis. This approach is based on the molecular imprint polymer (MIP) technique, which uses covalently immobilised A -42 to create specific detection cavities on the surface of singlewalled carbon nanotubes (SWCNTs). The biosensor was prepared by binding A -42 to the SWCNT surface and then imprinting it by adding acrylamide (monomer), N,N0-methylene-bis-acrylamide (crosslinker) and ammonium persulphate (initiator). The target peptide was removed from the polymer matrix by the proteolytic action of an enzyme (proteinase K). The presence of imprinting sites was confirmed by comparing a MIP-modified surface with a negative control (NIP) consisting of a similar material where the target molecule had been removed from the process. The ability of the sensing material to rebind A -42 was demonstrated by incorporating the MIP material as an electroactive compound in a PVC/plasticiser mixture applied to a solid conductive support of graphite. All steps of the synthesis of the imprinted materials were followed by Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR). The analytical performance was evaluated by potentiometric transduction, and the MIP material showed cationic slopes of 75 mV-decade1 in buffer pH 8.0 and a detection limit of 0.72 g/mL. Overall, potentiometric transduction confirmed that the sensor can discriminate A -42 in the presence of other biomolecules in the same solution. |
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Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detectionamyloid-42molecularly imprinted polymerspotentiometric sensorAlzheimer biomarkerThis paper presents a potentiometric biosensor for the detection of amyloid -42 (A -42) in point-of-care analysis. This approach is based on the molecular imprint polymer (MIP) technique, which uses covalently immobilised A -42 to create specific detection cavities on the surface of singlewalled carbon nanotubes (SWCNTs). The biosensor was prepared by binding A -42 to the SWCNT surface and then imprinting it by adding acrylamide (monomer), N,N0-methylene-bis-acrylamide (crosslinker) and ammonium persulphate (initiator). The target peptide was removed from the polymer matrix by the proteolytic action of an enzyme (proteinase K). The presence of imprinting sites was confirmed by comparing a MIP-modified surface with a negative control (NIP) consisting of a similar material where the target molecule had been removed from the process. The ability of the sensing material to rebind A -42 was demonstrated by incorporating the MIP material as an electroactive compound in a PVC/plasticiser mixture applied to a solid conductive support of graphite. All steps of the synthesis of the imprinted materials were followed by Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR). The analytical performance was evaluated by potentiometric transduction, and the MIP material showed cationic slopes of 75 mV-decade1 in buffer pH 8.0 and a detection limit of 0.72 g/mL. Overall, potentiometric transduction confirmed that the sensor can discriminate A -42 in the presence of other biomolecules in the same solution.2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/102840http://hdl.handle.net/10316/102840https://doi.org/10.3390/app12073625eng2076-3417Ribeiro, Sónia ClaúdiaFernandes, RúbenMoreira, Felismina T. C.Sales, Maria Goreti Ferreirainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-16T09:55:36Zoai:estudogeral.uc.pt:10316/102840Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:19:45.155673Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
spellingShingle |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection Ribeiro, Sónia Claúdia amyloid-42 molecularly imprinted polymers potentiometric sensor Alzheimer biomarker |
title_short |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title_full |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title_fullStr |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title_full_unstemmed |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title_sort |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
author |
Ribeiro, Sónia Claúdia |
author_facet |
Ribeiro, Sónia Claúdia Fernandes, Rúben Moreira, Felismina T. C. Sales, Maria Goreti Ferreira |
author_role |
author |
author2 |
Fernandes, Rúben Moreira, Felismina T. C. Sales, Maria Goreti Ferreira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Sónia Claúdia Fernandes, Rúben Moreira, Felismina T. C. Sales, Maria Goreti Ferreira |
dc.subject.por.fl_str_mv |
amyloid-42 molecularly imprinted polymers potentiometric sensor Alzheimer biomarker |
topic |
amyloid-42 molecularly imprinted polymers potentiometric sensor Alzheimer biomarker |
description |
This paper presents a potentiometric biosensor for the detection of amyloid -42 (A -42) in point-of-care analysis. This approach is based on the molecular imprint polymer (MIP) technique, which uses covalently immobilised A -42 to create specific detection cavities on the surface of singlewalled carbon nanotubes (SWCNTs). The biosensor was prepared by binding A -42 to the SWCNT surface and then imprinting it by adding acrylamide (monomer), N,N0-methylene-bis-acrylamide (crosslinker) and ammonium persulphate (initiator). The target peptide was removed from the polymer matrix by the proteolytic action of an enzyme (proteinase K). The presence of imprinting sites was confirmed by comparing a MIP-modified surface with a negative control (NIP) consisting of a similar material where the target molecule had been removed from the process. The ability of the sensing material to rebind A -42 was demonstrated by incorporating the MIP material as an electroactive compound in a PVC/plasticiser mixture applied to a solid conductive support of graphite. All steps of the synthesis of the imprinted materials were followed by Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR). The analytical performance was evaluated by potentiometric transduction, and the MIP material showed cationic slopes of 75 mV-decade1 in buffer pH 8.0 and a detection limit of 0.72 g/mL. Overall, potentiometric transduction confirmed that the sensor can discriminate A -42 in the presence of other biomolecules in the same solution. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/102840 http://hdl.handle.net/10316/102840 https://doi.org/10.3390/app12073625 |
url |
http://hdl.handle.net/10316/102840 https://doi.org/10.3390/app12073625 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2076-3417 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799134091406213120 |