Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.

Detalhes bibliográficos
Autor(a) principal: Sá-Nogueira, Isabel de
Data de Publicação: 2007
Outros Autores: Franco, Irina Saraiva, Mota, Luís Jaime, Soares, Cláudio Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/4204
Resumo: Nucleic Acid Research (2007) Vol.37 N. 14 4755-4766
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spelling Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.Nucleic Acid Research (2007) Vol.37 N. 14 4755-4766In the absence of arabinose, the AraR transcription factor represses the expression of genes involved in the utilization of arabinose, xylose and galactose in Bacillus subtilis. AraR exhibits a chimeric organization: the N-terminal DNA-binding region belongs to the GntR family and the C-terminal effector-binding domain is homologous to the GalR/LacI family. Here, the AraR-DNA-binding interactions were characterized in vivo and in vitro. The effect of residue substitutions in the AraR N-terminal domain and of base-pair exchanges into an AraR-DNA-binding operator site were examined by assaying for AraR-mediated regulatory activity in vivo and DNA-binding activity in vitro. The results showed that residues K4, R45 and Q61, located in or near the winged-helix DNA-binding motif, were the most critical amino acids required for AraR function. In addition, the analysis of the various mutations in an AraR palindromic operator sequence indicated that bases G9, A11 and T16 are crucial for AraR binding. Moreover, an AraR mutant M34T was isolated that partially suppressed the effect of mutations in the regulatory cis-elements. Together, these findings extend the knowledge on the nature of AraR nucleoprotein complexes and provide insight into the mechanism that underlies the mode of action of AraR and its orthologues.This work was partially supported by grants POCTI/BME/36164/00 and POCI/BIA-MIC/61140/04 from Fundacao para a Ciencia e Tecnologia (FCT) and FEDER to I.S.-N. I.S.F. is the holder of Ph.D. fellowship SFRH/BD/5233/01 from FCT. Funding to pay the Open Access publication charges for this article was provided by Fundacao para a Ciencia e Tecnologia.Oxford University PressRUNSá-Nogueira, Isabel deFranco, Irina SaraivaMota, Luís JaimeSoares, Cláudio Manuel2010-10-25T11:52:09Z2007-07-052007-07-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/4204engFranco. I.S. Mota. L.J. Soares. C.M. and de Sá-Nogueira I. (2007) Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon. Nucleic Acids Research 35: 4755–4766.0305-1048 (Print)info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:33:53Zoai:run.unl.pt:10362/4204Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:15:35.763204Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
title Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
spellingShingle Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
Sá-Nogueira, Isabel de
title_short Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
title_full Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
title_fullStr Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
title_full_unstemmed Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
title_sort Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
author Sá-Nogueira, Isabel de
author_facet Sá-Nogueira, Isabel de
Franco, Irina Saraiva
Mota, Luís Jaime
Soares, Cláudio Manuel
author_role author
author2 Franco, Irina Saraiva
Mota, Luís Jaime
Soares, Cláudio Manuel
author2_role author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Sá-Nogueira, Isabel de
Franco, Irina Saraiva
Mota, Luís Jaime
Soares, Cláudio Manuel
description Nucleic Acid Research (2007) Vol.37 N. 14 4755-4766
publishDate 2007
dc.date.none.fl_str_mv 2007-07-05
2007-07-05T00:00:00Z
2010-10-25T11:52:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/4204
url http://hdl.handle.net/10362/4204
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Franco. I.S. Mota. L.J. Soares. C.M. and de Sá-Nogueira I. (2007) Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon. Nucleic Acids Research 35: 4755–4766.
0305-1048 (Print)
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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