Biomolecular Fishing

Detalhes bibliográficos
Autor(a) principal: Furtado, Ana I.
Data de Publicação: 2023
Outros Autores: Viveiros, R., Bonifácio, Vasco D. B., Melo, André, Casimiro, T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/150940
Resumo: R.V. would like to acknowledge Individual Scientific Employment Stimulus (CEEC-IND), reference 2020.00377.CEECIND from the FCT/MCTES, Portugal. The Associate Laboratory Research Unit for Green Chemistry–Clean Technologies and Processes–LAQV-REQUIMTE is financed by national funds from FCT/MCTES UID/QUI/50006/2020 and cofinanced by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER–007265).
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spelling Biomolecular FishingDesign, Green Synthesis and Performance of L-Leucine-Molecularly Imprinted PolymersR.V. would like to acknowledge Individual Scientific Employment Stimulus (CEEC-IND), reference 2020.00377.CEECIND from the FCT/MCTES, Portugal. The Associate Laboratory Research Unit for Green Chemistry–Clean Technologies and Processes–LAQV-REQUIMTE is financed by national funds from FCT/MCTES UID/QUI/50006/2020 and cofinanced by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER–007265).Biopurification is a challenging and growing market. Despite great efforts in the past years, current purification strategies still lack specificity, efficiency, and cost-effectiveness. The development of more sustainable functional materials and processes needs to address pressing environmental goals, efficiency, scale-up, and cost. Herein, l-leucine (LEU)-molecularly imprinted polymers (MIPs), LEU-MIPs, are presented as novel biomolecular fishing polymers for affinity sustainable biopurification. Rational design was performed using quantum mechanics calculations and molecular modeling for selecting the most appropriate monomers. LEU-MIPs were synthesized for the first time by two different green approaches, supercritical carbon dioxide (scCO2) technology and mechanochemistry. A significant imprinting factor of 12 and a binding capacity of 27 mg LEU/g polymer were obtained for the LEU-MIP synthesized in scCO2 using 2-vinylpyridine as a functional monomer, while the LEU-MIP using acrylamide as a functional monomer synthesized by mechanochemistry showed an imprinting factor of 1.4 and a binding capacity of 18 mg LEU/g polymer, both systems operating at a low binding concentration (0.5 mg LEU/mL) under physiological conditions. As expected, at a higher concentration (1.5 mg LEU/mL), the binding capacity was considerably increased. Both green technologies show high potential in obtaining ready-to-use, stable, and low-cost polymers with a molecular recognition ability for target biomolecules, being promising materials for biopurification processes.DQ - Departamento de QuímicaLAQV@REQUIMTERUNFurtado, Ana I.Viveiros, R.Bonifácio, Vasco D. B.Melo, AndréCasimiro, T.2023-03-20T22:20:56Z2023-03-012023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://hdl.handle.net/10362/150940eng2637-6105PURE: 44935243https://doi.org/10.1021/acsomega.2c05714info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:33:20Zoai:run.unl.pt:10362/150940Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:22.246767Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biomolecular Fishing
Design, Green Synthesis and Performance of L-Leucine-Molecularly Imprinted Polymers
title Biomolecular Fishing
spellingShingle Biomolecular Fishing
Furtado, Ana I.
title_short Biomolecular Fishing
title_full Biomolecular Fishing
title_fullStr Biomolecular Fishing
title_full_unstemmed Biomolecular Fishing
title_sort Biomolecular Fishing
author Furtado, Ana I.
author_facet Furtado, Ana I.
Viveiros, R.
Bonifácio, Vasco D. B.
Melo, André
Casimiro, T.
author_role author
author2 Viveiros, R.
Bonifácio, Vasco D. B.
Melo, André
Casimiro, T.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
LAQV@REQUIMTE
RUN
dc.contributor.author.fl_str_mv Furtado, Ana I.
Viveiros, R.
Bonifácio, Vasco D. B.
Melo, André
Casimiro, T.
description R.V. would like to acknowledge Individual Scientific Employment Stimulus (CEEC-IND), reference 2020.00377.CEECIND from the FCT/MCTES, Portugal. The Associate Laboratory Research Unit for Green Chemistry–Clean Technologies and Processes–LAQV-REQUIMTE is financed by national funds from FCT/MCTES UID/QUI/50006/2020 and cofinanced by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER–007265).
publishDate 2023
dc.date.none.fl_str_mv 2023-03-20T22:20:56Z
2023-03-01
2023-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/150940
url http://hdl.handle.net/10362/150940
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2637-6105
PURE: 44935243
https://doi.org/10.1021/acsomega.2c05714
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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