Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction

Detalhes bibliográficos
Autor(a) principal: Majid, Hafiz
Data de Publicação: 2021
Outros Autores: Silva, Filipa V.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23792
Resumo: Alzheimer’s disease (AD) is a neurodegenerative disease leading to irreversible neuronaldamage. Kanuka or Kunzea ericoides (A. Richard) revealed a remarkable inhibition of B-secretase activity, an important enzyme for AD. This study presents optimization of theultrasound assisted extraction (UAE) of inhibitors of B-secretase and antioxidants from K.ericoides leaves, and yield by response surface methodology (RSM). Experimental validationof optimized conditions and 50% inhibitory extract concentrations (IC50) determinationswere performed. The extraction time and temperature were significant for enzyme inhibition, RSA (DPPH radical scavenging activity) and yield, while acoustic power density had lesseffect on the 3 responses. The optimum conditions were 15.6 min extraction time, 69◦C and0.43 W/mL. The ultrasound extraction produced better extracts in term of enzyme inhibition and RSA (lower IC50: 14.25 ug/mL enzyme, 3.17 ug/mL RSA), in comparison to Soxhletand maceration combined with heat, and a more rapid extraction with increased yield ofextraction. Ultrasound kanuka extracts can potentially complement existing AD treatment
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spelling Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extractionKunzea ericoidesplant extractsoptimizationenzyme inhibitionBACE1ultrasound extractionAlzheimer’s disease (AD) is a neurodegenerative disease leading to irreversible neuronaldamage. Kanuka or Kunzea ericoides (A. Richard) revealed a remarkable inhibition of B-secretase activity, an important enzyme for AD. This study presents optimization of theultrasound assisted extraction (UAE) of inhibitors of B-secretase and antioxidants from K.ericoides leaves, and yield by response surface methodology (RSM). Experimental validationof optimized conditions and 50% inhibitory extract concentrations (IC50) determinationswere performed. The extraction time and temperature were significant for enzyme inhibition, RSA (DPPH radical scavenging activity) and yield, while acoustic power density had lesseffect on the 3 responses. The optimum conditions were 15.6 min extraction time, 69◦C and0.43 W/mL. The ultrasound extraction produced better extracts in term of enzyme inhibition and RSA (lower IC50: 14.25 ug/mL enzyme, 3.17 ug/mL RSA), in comparison to Soxhletand maceration combined with heat, and a more rapid extraction with increased yield ofextraction. Ultrasound kanuka extracts can potentially complement existing AD treatmentElsevierRepositório da Universidade de LisboaMajid, HafizSilva, Filipa V.M.2022-03-15T11:45:07Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23792engMajid H, Silva FVM (2021). Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction. Food and Bioproducts Processing, 128:109-120https://doi.org/10.1016/j.fbp.2021.04.018info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:25Zoai:www.repository.utl.pt:10400.5/23792Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:56.242883Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction
title Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction
spellingShingle Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction
Majid, Hafiz
Kunzea ericoides
plant extracts
optimization
enzyme inhibition
BACE1
ultrasound extraction
title_short Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction
title_full Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction
title_fullStr Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction
title_full_unstemmed Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction
title_sort Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction
author Majid, Hafiz
author_facet Majid, Hafiz
Silva, Filipa V.M.
author_role author
author2 Silva, Filipa V.M.
author2_role author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Majid, Hafiz
Silva, Filipa V.M.
dc.subject.por.fl_str_mv Kunzea ericoides
plant extracts
optimization
enzyme inhibition
BACE1
ultrasound extraction
topic Kunzea ericoides
plant extracts
optimization
enzyme inhibition
BACE1
ultrasound extraction
description Alzheimer’s disease (AD) is a neurodegenerative disease leading to irreversible neuronaldamage. Kanuka or Kunzea ericoides (A. Richard) revealed a remarkable inhibition of B-secretase activity, an important enzyme for AD. This study presents optimization of theultrasound assisted extraction (UAE) of inhibitors of B-secretase and antioxidants from K.ericoides leaves, and yield by response surface methodology (RSM). Experimental validationof optimized conditions and 50% inhibitory extract concentrations (IC50) determinationswere performed. The extraction time and temperature were significant for enzyme inhibition, RSA (DPPH radical scavenging activity) and yield, while acoustic power density had lesseffect on the 3 responses. The optimum conditions were 15.6 min extraction time, 69◦C and0.43 W/mL. The ultrasound extraction produced better extracts in term of enzyme inhibition and RSA (lower IC50: 14.25 ug/mL enzyme, 3.17 ug/mL RSA), in comparison to Soxhletand maceration combined with heat, and a more rapid extraction with increased yield ofextraction. Ultrasound kanuka extracts can potentially complement existing AD treatment
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2022-03-15T11:45:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23792
url http://hdl.handle.net/10400.5/23792
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Majid H, Silva FVM (2021). Kanuka bush leaves for Alzheimer's disease: Improved inhibition of β-secretase enzyme, antioxidant capacity and yield of extracts by ultrasound assisted extraction. Food and Bioproducts Processing, 128:109-120
https://doi.org/10.1016/j.fbp.2021.04.018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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