Silk-based anisotropical 3D biotextiles for bone regeneration
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/23052 |
Resumo: | Bone loss in the craniofacial complex can been treated using several conventional therapeutic strategies that face many obstacles and limitations. In this work, novel three-dimensional (3D) biotextile architectures were developed as a possible strategy for flat bone regeneration applications. As a fully automated processing route, this strategy as potential to be easily industrialized. Silk fibroin (SF) yarns were processed into weft-knitted fabrics spaced by a monofilament of polyethylene terephthalate (PET). A comparative study with a similar 3D structure made entirely of PET was established. Highly porous scaffolds with homogeneous pore distribution were observed using micro-computed tomography analysis. The wet state dynamic mechanical analysis revealed a storage modulus In the frequency range tested, the storage modulus values obtained for SF-PET scaffolds were higher than for the PET scaffolds. Human adipose-derived stem cells (hASCs) cultured on the SF-PET spacer structures showed the typical pattern for ALP activity under osteogenic culture conditions. Osteogenic differentiation of hASCs on SF PET and PET constructs was also observed by extracellular matrix mineralization and expression of osteogenic-related markers (osteocalcin, osteopontin and collagen type I) after 28 days of osteogenic culture, in comparison to the control basal medium. The quantification of convergent macroscopic blood vessels toward the scaffolds by a chick chorioallantoic membrane assay, showed higher angiogenic response induced by the SF-PET textile scaffolds than PET structures and gelatin sponge controls. Subcutaneous implantation in CD-1 mice revealed tissue ingrowth's accompanied by blood vessels infiltration in both spacer constructs. The structural adaptability of textile structures combined to the structural similarities of the 3D knitted spacer fabrics to craniofacial bone tissue and achieved biological performance, make these scaffolds a possible solution for tissue engineering approaches in this area. |
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Silk-based anisotropical 3D biotextiles for bone regenerationBiotextileTextileSilk FibroinKnitted Spacer FabricsHuman Adipose-Derived Stem CellsCraniofacial Bone Tissue EngineeringBone loss in the craniofacial complex can been treated using several conventional therapeutic strategies that face many obstacles and limitations. In this work, novel three-dimensional (3D) biotextile architectures were developed as a possible strategy for flat bone regeneration applications. As a fully automated processing route, this strategy as potential to be easily industrialized. Silk fibroin (SF) yarns were processed into weft-knitted fabrics spaced by a monofilament of polyethylene terephthalate (PET). A comparative study with a similar 3D structure made entirely of PET was established. Highly porous scaffolds with homogeneous pore distribution were observed using micro-computed tomography analysis. The wet state dynamic mechanical analysis revealed a storage modulus In the frequency range tested, the storage modulus values obtained for SF-PET scaffolds were higher than for the PET scaffolds. Human adipose-derived stem cells (hASCs) cultured on the SF-PET spacer structures showed the typical pattern for ALP activity under osteogenic culture conditions. Osteogenic differentiation of hASCs on SF PET and PET constructs was also observed by extracellular matrix mineralization and expression of osteogenic-related markers (osteocalcin, osteopontin and collagen type I) after 28 days of osteogenic culture, in comparison to the control basal medium. The quantification of convergent macroscopic blood vessels toward the scaffolds by a chick chorioallantoic membrane assay, showed higher angiogenic response induced by the SF-PET textile scaffolds than PET structures and gelatin sponge controls. Subcutaneous implantation in CD-1 mice revealed tissue ingrowth's accompanied by blood vessels infiltration in both spacer constructs. The structural adaptability of textile structures combined to the structural similarities of the 3D knitted spacer fabrics to craniofacial bone tissue and achieved biological performance, make these scaffolds a possible solution for tissue engineering approaches in this area.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaRibeiro, Viviana P.Silva-Correia, JoanaNascimento, Ana I.Morais, Alain da SilvaMarques, Alexandra P.Ribeiro, Ana S.Silva, Carla J.Bonifácio, GrataSousa, Rui A.Oliveira, Joaquim M.Oliveira, Ana L.Reis, Rui L.2020-01-15T01:30:53Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/23052engRIBEIRO, V.P.; SILVA-CORREIA, J.; NASCIMENTO, A.I.; MORAIS, A. DA SILVA; MARQUES, A.P.; RIBEIRO, A.S.; SILVA, C.J.; BONIFÁCIO, G.; SOUSA, R.A.; OLIVEIRA, J. M.; OLIVEIRA, Ana L.; REIS, R.L. (2017). Silk-Based Anisotropical 3D Biotextiles for Bone Regeneration. Biomaterials, 123, 92-1060142-961210.1016/j.biomaterials.2017.01.0271878-59058501227360628161684000395597800008info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-12T01:38:03Zoai:repositorio.ucp.pt:10400.14/23052Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:17:46.104865Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Silk-based anisotropical 3D biotextiles for bone regeneration |
title |
Silk-based anisotropical 3D biotextiles for bone regeneration |
spellingShingle |
Silk-based anisotropical 3D biotextiles for bone regeneration Ribeiro, Viviana P. Biotextile Textile Silk Fibroin Knitted Spacer Fabrics Human Adipose-Derived Stem Cells Craniofacial Bone Tissue Engineering |
title_short |
Silk-based anisotropical 3D biotextiles for bone regeneration |
title_full |
Silk-based anisotropical 3D biotextiles for bone regeneration |
title_fullStr |
Silk-based anisotropical 3D biotextiles for bone regeneration |
title_full_unstemmed |
Silk-based anisotropical 3D biotextiles for bone regeneration |
title_sort |
Silk-based anisotropical 3D biotextiles for bone regeneration |
author |
Ribeiro, Viviana P. |
author_facet |
Ribeiro, Viviana P. Silva-Correia, Joana Nascimento, Ana I. Morais, Alain da Silva Marques, Alexandra P. Ribeiro, Ana S. Silva, Carla J. Bonifácio, Grata Sousa, Rui A. Oliveira, Joaquim M. Oliveira, Ana L. Reis, Rui L. |
author_role |
author |
author2 |
Silva-Correia, Joana Nascimento, Ana I. Morais, Alain da Silva Marques, Alexandra P. Ribeiro, Ana S. Silva, Carla J. Bonifácio, Grata Sousa, Rui A. Oliveira, Joaquim M. Oliveira, Ana L. Reis, Rui L. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Ribeiro, Viviana P. Silva-Correia, Joana Nascimento, Ana I. Morais, Alain da Silva Marques, Alexandra P. Ribeiro, Ana S. Silva, Carla J. Bonifácio, Grata Sousa, Rui A. Oliveira, Joaquim M. Oliveira, Ana L. Reis, Rui L. |
dc.subject.por.fl_str_mv |
Biotextile Textile Silk Fibroin Knitted Spacer Fabrics Human Adipose-Derived Stem Cells Craniofacial Bone Tissue Engineering |
topic |
Biotextile Textile Silk Fibroin Knitted Spacer Fabrics Human Adipose-Derived Stem Cells Craniofacial Bone Tissue Engineering |
description |
Bone loss in the craniofacial complex can been treated using several conventional therapeutic strategies that face many obstacles and limitations. In this work, novel three-dimensional (3D) biotextile architectures were developed as a possible strategy for flat bone regeneration applications. As a fully automated processing route, this strategy as potential to be easily industrialized. Silk fibroin (SF) yarns were processed into weft-knitted fabrics spaced by a monofilament of polyethylene terephthalate (PET). A comparative study with a similar 3D structure made entirely of PET was established. Highly porous scaffolds with homogeneous pore distribution were observed using micro-computed tomography analysis. The wet state dynamic mechanical analysis revealed a storage modulus In the frequency range tested, the storage modulus values obtained for SF-PET scaffolds were higher than for the PET scaffolds. Human adipose-derived stem cells (hASCs) cultured on the SF-PET spacer structures showed the typical pattern for ALP activity under osteogenic culture conditions. Osteogenic differentiation of hASCs on SF PET and PET constructs was also observed by extracellular matrix mineralization and expression of osteogenic-related markers (osteocalcin, osteopontin and collagen type I) after 28 days of osteogenic culture, in comparison to the control basal medium. The quantification of convergent macroscopic blood vessels toward the scaffolds by a chick chorioallantoic membrane assay, showed higher angiogenic response induced by the SF-PET textile scaffolds than PET structures and gelatin sponge controls. Subcutaneous implantation in CD-1 mice revealed tissue ingrowth's accompanied by blood vessels infiltration in both spacer constructs. The structural adaptability of textile structures combined to the structural similarities of the 3D knitted spacer fabrics to craniofacial bone tissue and achieved biological performance, make these scaffolds a possible solution for tissue engineering approaches in this area. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z 2020-01-15T01:30:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/23052 |
url |
http://hdl.handle.net/10400.14/23052 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
RIBEIRO, V.P.; SILVA-CORREIA, J.; NASCIMENTO, A.I.; MORAIS, A. DA SILVA; MARQUES, A.P.; RIBEIRO, A.S.; SILVA, C.J.; BONIFÁCIO, G.; SOUSA, R.A.; OLIVEIRA, J. M.; OLIVEIRA, Ana L.; REIS, R.L. (2017). Silk-Based Anisotropical 3D Biotextiles for Bone Regeneration. Biomaterials, 123, 92-106 0142-9612 10.1016/j.biomaterials.2017.01.027 1878-5905 85012273606 28161684 000395597800008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131866191626240 |