Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin

Detalhes bibliográficos
Autor(a) principal: Canteiro, Beatriz
Data de Publicação: 2023
Outros Autores: Mendes, Maria, Jacques, Filipa, Delgadinho, Mariana, Oliveira, Ketlyn, Ginete, Catarina, Gomes, Mário, Ribeiro, Edna, Brito, Miguel, Gomes, Anita Q.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/16390
Resumo: IDI&CA-IPL/2022/miRCa/ESTeSL
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spelling Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobinSickle cell diseaseReactivation of fetal hemoglobinQuercetinFlavonoidHydroxyureaHbF regulatory/silencing genesIDI&CA-IPL/2022/miRCa/ESTeSLIDI&CA-IPL/2022/miRCa/ESTeSLSickle Cell anemia (SCA) is a hereditary hemoglobinopathy with the formation of hemoglobin S, associated with severe health outcomes. Currently, induction of fetal hemoglobin (HbF) is one of the most promising therapeutic strategies. Here we aimed to assess the potential of the natural compound Quercetin, in transcriptional expression of globin and HbF regulatory/silencing genes. In this study, the K562 cell line was used as an SCA model. Cells were exposed to Quercetin at final concentrations of 0.2 and 20 µM, and Hydroxyurea (25 µg/mL) was used as a positive control. Cell viability and proliferation were assessed through trypan blue exclusion assay. Transcriptional expression was performed by RT-qPCR using specific primers. Significant differences were analyzed using a t-test. No cytotoxic effects were observed following exposure to Quercetin. The transcriptional analysis demonstrated that Quercetin affects mRNA levels of HbF regulatory/silencing genes with associated downregulation of BCL11A, MYB, KLF1, and HBB and upregulation of HBG and BGLT3, as well as alterations in the expression of miRNAs involved in HbF post-transcriptional regulation. Our results sustain Quercetin's potential as an HbF inducer with associated upregulation of HbF-activators and decreased expression of HbF-inhibitors. These data support the need for further studies in order to confirm the potential of this compound as a new therapeutic option for β -hemoglobinopathies in the future.RCIPLCanteiro, BeatrizMendes, MariaJacques, FilipaDelgadinho, MarianaOliveira, KetlynGinete, CatarinaGomes, MárioRibeiro, EdnaBrito, MiguelGomes, Anita Q.2023-062023-06-01T00:00:00Z2025-08-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/16390engCanteiro B, Mendes M, Jacques F, Delgadinho M, Oliveira K, Ginete C, Gomes M, Ribeiro E, Brito M, Gomes AQ. Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin. In: ENBENG 2023 – 7th IEEE Portuguese Meeting on Bioengineering, Porto, June 22-23, 2023. p. 140-3.10.1109/ENBENG58165.2023.10175363info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-30T02:15:46Zoai:repositorio.ipl.pt:10400.21/16390Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:27:43.187932Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin
title Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin
spellingShingle Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin
Canteiro, Beatriz
Sickle cell disease
Reactivation of fetal hemoglobin
Quercetin
Flavonoid
Hydroxyurea
HbF regulatory/silencing genes
IDI&CA-IPL/2022/miRCa/ESTeSL
title_short Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin
title_full Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin
title_fullStr Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin
title_full_unstemmed Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin
title_sort Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin
author Canteiro, Beatriz
author_facet Canteiro, Beatriz
Mendes, Maria
Jacques, Filipa
Delgadinho, Mariana
Oliveira, Ketlyn
Ginete, Catarina
Gomes, Mário
Ribeiro, Edna
Brito, Miguel
Gomes, Anita Q.
author_role author
author2 Mendes, Maria
Jacques, Filipa
Delgadinho, Mariana
Oliveira, Ketlyn
Ginete, Catarina
Gomes, Mário
Ribeiro, Edna
Brito, Miguel
Gomes, Anita Q.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Canteiro, Beatriz
Mendes, Maria
Jacques, Filipa
Delgadinho, Mariana
Oliveira, Ketlyn
Ginete, Catarina
Gomes, Mário
Ribeiro, Edna
Brito, Miguel
Gomes, Anita Q.
dc.subject.por.fl_str_mv Sickle cell disease
Reactivation of fetal hemoglobin
Quercetin
Flavonoid
Hydroxyurea
HbF regulatory/silencing genes
IDI&CA-IPL/2022/miRCa/ESTeSL
topic Sickle cell disease
Reactivation of fetal hemoglobin
Quercetin
Flavonoid
Hydroxyurea
HbF regulatory/silencing genes
IDI&CA-IPL/2022/miRCa/ESTeSL
description IDI&CA-IPL/2022/miRCa/ESTeSL
publishDate 2023
dc.date.none.fl_str_mv 2023-06
2023-06-01T00:00:00Z
2025-08-23T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/16390
url http://hdl.handle.net/10400.21/16390
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Canteiro B, Mendes M, Jacques F, Delgadinho M, Oliveira K, Ginete C, Gomes M, Ribeiro E, Brito M, Gomes AQ. Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin. In: ENBENG 2023 – 7th IEEE Portuguese Meeting on Bioengineering, Porto, June 22-23, 2023. p. 140-3.
10.1109/ENBENG58165.2023.10175363
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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