Identification and antimicrobial resistance profile of bacteria isolated from the
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/33715 |
Resumo: | The main cause of infertility in mares is endometritis, characterized by acute or chronic inflammation of the endometrium. One of the main causes of the occurrence of inflammation in the endometrium is the response to bacterial infection. When the infection overcomes the defense capacity of the host it leads to the development of bacterial endometritis, often caused by Streptococcus equi subsp. zooepidemicus, an opportunistic pathogen. The present study aimed to evaluate the presence of bacteria in the uterus of mares before insemination. For this purpose, uterine washings were performed with sterile saline solution. Samples were centrifuged at 8000 g for 10min at 4oC, and the pellet was streaked onto Blood Agar and MacConkey plates. The obtained isolates were identified using biochemical (VITEK 2 Compac and API, Biomerieux) and molecular identification methodologies (16S rRNA gene sequencing). Moreover, antimicrobial susceptibility tests (AST) were performed with VITEK 2 Compac, for fast growing bacteria and disc diffusion method, for fastidious bacteria. A total of 62 uterine washings were analyzed. A positive culture was obtained in 66% of the specimens, resulting in 57 isolates, with 57% of Gram-positive bacteria isolated. Regarding prevalence, the most frequently isolated genera were Streptococcus (33%), Escherichia (25%) and Staphylococcus (18%), while the most frequent species was Escherichia coli, followed by S. equi subsp. zooepidemicus. Most Gram-positive bacteria were sensitive to the following three antimicrobials, namely tetracycline, ceftiofur, and enrofloxacin. Regarding Gram-negative bacteria, over 90% of the isolates were sensitive to ceftiofur and gentamycin, while over 60% of the isolates were sensitive to enrofloxacin. |
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Identification and antimicrobial resistance profile of bacteria isolated from theantimicrobial resistancemaresThe main cause of infertility in mares is endometritis, characterized by acute or chronic inflammation of the endometrium. One of the main causes of the occurrence of inflammation in the endometrium is the response to bacterial infection. When the infection overcomes the defense capacity of the host it leads to the development of bacterial endometritis, often caused by Streptococcus equi subsp. zooepidemicus, an opportunistic pathogen. The present study aimed to evaluate the presence of bacteria in the uterus of mares before insemination. For this purpose, uterine washings were performed with sterile saline solution. Samples were centrifuged at 8000 g for 10min at 4oC, and the pellet was streaked onto Blood Agar and MacConkey plates. The obtained isolates were identified using biochemical (VITEK 2 Compac and API, Biomerieux) and molecular identification methodologies (16S rRNA gene sequencing). Moreover, antimicrobial susceptibility tests (AST) were performed with VITEK 2 Compac, for fast growing bacteria and disc diffusion method, for fastidious bacteria. A total of 62 uterine washings were analyzed. A positive culture was obtained in 66% of the specimens, resulting in 57 isolates, with 57% of Gram-positive bacteria isolated. Regarding prevalence, the most frequently isolated genera were Streptococcus (33%), Escherichia (25%) and Staphylococcus (18%), while the most frequent species was Escherichia coli, followed by S. equi subsp. zooepidemicus. Most Gram-positive bacteria were sensitive to the following three antimicrobials, namely tetracycline, ceftiofur, and enrofloxacin. Regarding Gram-negative bacteria, over 90% of the isolates were sensitive to ceftiofur and gentamycin, while over 60% of the isolates were sensitive to enrofloxacin.UE – Universidade de Évora2023-01-30T16:04:50Z2023-01-302022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/33715http://hdl.handle.net/10174/33715porConceição, S., Bessa de Carvalho, I., Guimarães, H., Queiroga, C., Laranjo, M., Bettencourt, E. (2022) “Identification and antimicrobial resistance profile of bacteria isolated from the uterus of mares”. VII Encontro de Estudantes de Doutoramento em Ambiente e Agricultura. Universidade de Évora, Évora, Portugal, 12 e 13 de Dezembro. Livro de Resumos p. 14.naonaosimsmpc@uevora.ptinesbessadecarvalho@gmail.comhguimaraes@sapo.ptcrique@uevora.ptmlaranjo@uevora.ptemvb@uevora.pt384Conceição, SBessa de Carvalho, IGuimarães, HQueiroga, MCLaranjo, MBettencourt, Einfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:35:44Zoai:dspace.uevora.pt:10174/33715Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:22:29.653772Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Identification and antimicrobial resistance profile of bacteria isolated from the |
title |
Identification and antimicrobial resistance profile of bacteria isolated from the |
spellingShingle |
Identification and antimicrobial resistance profile of bacteria isolated from the Conceição, S antimicrobial resistance mares |
title_short |
Identification and antimicrobial resistance profile of bacteria isolated from the |
title_full |
Identification and antimicrobial resistance profile of bacteria isolated from the |
title_fullStr |
Identification and antimicrobial resistance profile of bacteria isolated from the |
title_full_unstemmed |
Identification and antimicrobial resistance profile of bacteria isolated from the |
title_sort |
Identification and antimicrobial resistance profile of bacteria isolated from the |
author |
Conceição, S |
author_facet |
Conceição, S Bessa de Carvalho, I Guimarães, H Queiroga, MC Laranjo, M Bettencourt, E |
author_role |
author |
author2 |
Bessa de Carvalho, I Guimarães, H Queiroga, MC Laranjo, M Bettencourt, E |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Conceição, S Bessa de Carvalho, I Guimarães, H Queiroga, MC Laranjo, M Bettencourt, E |
dc.subject.por.fl_str_mv |
antimicrobial resistance mares |
topic |
antimicrobial resistance mares |
description |
The main cause of infertility in mares is endometritis, characterized by acute or chronic inflammation of the endometrium. One of the main causes of the occurrence of inflammation in the endometrium is the response to bacterial infection. When the infection overcomes the defense capacity of the host it leads to the development of bacterial endometritis, often caused by Streptococcus equi subsp. zooepidemicus, an opportunistic pathogen. The present study aimed to evaluate the presence of bacteria in the uterus of mares before insemination. For this purpose, uterine washings were performed with sterile saline solution. Samples were centrifuged at 8000 g for 10min at 4oC, and the pellet was streaked onto Blood Agar and MacConkey plates. The obtained isolates were identified using biochemical (VITEK 2 Compac and API, Biomerieux) and molecular identification methodologies (16S rRNA gene sequencing). Moreover, antimicrobial susceptibility tests (AST) were performed with VITEK 2 Compac, for fast growing bacteria and disc diffusion method, for fastidious bacteria. A total of 62 uterine washings were analyzed. A positive culture was obtained in 66% of the specimens, resulting in 57 isolates, with 57% of Gram-positive bacteria isolated. Regarding prevalence, the most frequently isolated genera were Streptococcus (33%), Escherichia (25%) and Staphylococcus (18%), while the most frequent species was Escherichia coli, followed by S. equi subsp. zooepidemicus. Most Gram-positive bacteria were sensitive to the following three antimicrobials, namely tetracycline, ceftiofur, and enrofloxacin. Regarding Gram-negative bacteria, over 90% of the isolates were sensitive to ceftiofur and gentamycin, while over 60% of the isolates were sensitive to enrofloxacin. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01T00:00:00Z 2023-01-30T16:04:50Z 2023-01-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/33715 http://hdl.handle.net/10174/33715 |
url |
http://hdl.handle.net/10174/33715 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Conceição, S., Bessa de Carvalho, I., Guimarães, H., Queiroga, C., Laranjo, M., Bettencourt, E. (2022) “Identification and antimicrobial resistance profile of bacteria isolated from the uterus of mares”. VII Encontro de Estudantes de Doutoramento em Ambiente e Agricultura. Universidade de Évora, Évora, Portugal, 12 e 13 de Dezembro. Livro de Resumos p. 14. nao nao sim smpc@uevora.pt inesbessadecarvalho@gmail.com hguimaraes@sapo.pt crique@uevora.pt mlaranjo@uevora.pt emvb@uevora.pt 384 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
UE – Universidade de Évora |
publisher.none.fl_str_mv |
UE – Universidade de Évora |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136706436268032 |