Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-018X2018000100022 |
Resumo: | Plant-parasitic nematodes are highly damaging pests in many crops of great economic importance. A substantial part of this damage is caused by root-knot nematodes (RKN), Meloidogyne spp. represents losses of millions of euros, due to their wide geographical distribution and range of host plants. Therefore, an accurate and reliable identification is needed to establish effective, sustainable and environmentally safe control measures. The main goal of this study was to characterise morphological, biochemical and molecularly one Portuguese isolate of Meloidogyne found associated with tomato crop. Morphometric studies were based on second-stage juveniles (body length, stylet length, tail length, hyaline terminus length and DGO) and female perineal patterns. Biochemical assays using esterases (EST isozymes) were performed on females and the PAGE enzymatic patterns compared to previous descriptions. Molecular analysis was based on PCR using the species-specific SCAR primers Fjav/Rjav. Results indicated the presence of M. javanica. This study confirms the need of using the three approaches for an accurate and effective RKN diagnosis. |
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Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomatoesterase phenotypeMeloidogyne spp.molecular identificationperineal patternstomatoPlant-parasitic nematodes are highly damaging pests in many crops of great economic importance. A substantial part of this damage is caused by root-knot nematodes (RKN), Meloidogyne spp. represents losses of millions of euros, due to their wide geographical distribution and range of host plants. Therefore, an accurate and reliable identification is needed to establish effective, sustainable and environmentally safe control measures. The main goal of this study was to characterise morphological, biochemical and molecularly one Portuguese isolate of Meloidogyne found associated with tomato crop. Morphometric studies were based on second-stage juveniles (body length, stylet length, tail length, hyaline terminus length and DGO) and female perineal patterns. Biochemical assays using esterases (EST isozymes) were performed on females and the PAGE enzymatic patterns compared to previous descriptions. Molecular analysis was based on PCR using the species-specific SCAR primers Fjav/Rjav. Results indicated the presence of M. javanica. This study confirms the need of using the three approaches for an accurate and effective RKN diagnosis.Sociedade de Ciências Agrárias de Portugal2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-018X2018000100022Revista de Ciências Agrárias v.41 n.1 2018reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-018X2018000100022Rusinque,LeidyInácio,M. LurdesMota,MarianaNóbrega,Filomenainfo:eu-repo/semantics/openAccess2024-02-06T17:02:30Zoai:scielo:S0871-018X2018000100022Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:17:32.072322Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato |
title |
Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato |
spellingShingle |
Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato Rusinque,Leidy esterase phenotype Meloidogyne spp. molecular identification perineal patterns tomato |
title_short |
Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato |
title_full |
Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato |
title_fullStr |
Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato |
title_full_unstemmed |
Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato |
title_sort |
Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato |
author |
Rusinque,Leidy |
author_facet |
Rusinque,Leidy Inácio,M. Lurdes Mota,Mariana Nóbrega,Filomena |
author_role |
author |
author2 |
Inácio,M. Lurdes Mota,Mariana Nóbrega,Filomena |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Rusinque,Leidy Inácio,M. Lurdes Mota,Mariana Nóbrega,Filomena |
dc.subject.por.fl_str_mv |
esterase phenotype Meloidogyne spp. molecular identification perineal patterns tomato |
topic |
esterase phenotype Meloidogyne spp. molecular identification perineal patterns tomato |
description |
Plant-parasitic nematodes are highly damaging pests in many crops of great economic importance. A substantial part of this damage is caused by root-knot nematodes (RKN), Meloidogyne spp. represents losses of millions of euros, due to their wide geographical distribution and range of host plants. Therefore, an accurate and reliable identification is needed to establish effective, sustainable and environmentally safe control measures. The main goal of this study was to characterise morphological, biochemical and molecularly one Portuguese isolate of Meloidogyne found associated with tomato crop. Morphometric studies were based on second-stage juveniles (body length, stylet length, tail length, hyaline terminus length and DGO) and female perineal patterns. Biochemical assays using esterases (EST isozymes) were performed on females and the PAGE enzymatic patterns compared to previous descriptions. Molecular analysis was based on PCR using the species-specific SCAR primers Fjav/Rjav. Results indicated the presence of M. javanica. This study confirms the need of using the three approaches for an accurate and effective RKN diagnosis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-018X2018000100022 |
url |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-018X2018000100022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-018X2018000100022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
dc.source.none.fl_str_mv |
Revista de Ciências Agrárias v.41 n.1 2018 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799137269087469568 |