Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400019 |
Resumo: | The enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 10² CFU.mL-1. |
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Food Science and Technology (Campinas) |
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Standardization of a multiplex PCR for the identification of coagulase-positive StaphylococcusS. aureusS. hyicusS. intermediusPCRnuc geneThe enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 10² CFU.mL-1.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400019Food Science and Technology v.31 n.4 2011reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/S0101-20612011000400019info:eu-repo/semantics/openAccessGandra,Eliezer AvilaFernandez,Maria AparecidaSilva,Jorge AdolfoSilva,Wladimir Padilha daeng2012-02-06T00:00:00Zoai:scielo:S0101-20612011000400019Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2012-02-06T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus |
title |
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus |
spellingShingle |
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus Gandra,Eliezer Avila S. aureus S. hyicus S. intermedius PCR nuc gene |
title_short |
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus |
title_full |
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus |
title_fullStr |
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus |
title_full_unstemmed |
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus |
title_sort |
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus |
author |
Gandra,Eliezer Avila |
author_facet |
Gandra,Eliezer Avila Fernandez,Maria Aparecida Silva,Jorge Adolfo Silva,Wladimir Padilha da |
author_role |
author |
author2 |
Fernandez,Maria Aparecida Silva,Jorge Adolfo Silva,Wladimir Padilha da |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Gandra,Eliezer Avila Fernandez,Maria Aparecida Silva,Jorge Adolfo Silva,Wladimir Padilha da |
dc.subject.por.fl_str_mv |
S. aureus S. hyicus S. intermedius PCR nuc gene |
topic |
S. aureus S. hyicus S. intermedius PCR nuc gene |
description |
The enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 10² CFU.mL-1. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0101-20612011000400019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.31 n.4 2011 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126316568641536 |