Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101242 |
Resumo: | Abstract To investigate the effects of quercetin on the expression of microRNA-155 (miR-155) and the functions of lipopolysaccharide (LPS)-treated extravillous trophoblast cells. Cell apoptosis significantly increased and invasion cell number and wound healing rate significantly decreased in the model group compared with the NC group (P < 0.001). Quercetin supplement significantly improved the cell apoptosis rate, invasion cell number and wound healing rate (P < 0.05, respectively) compared with the model group. However, after miR-155 transfection, the cell apoptosis rate, invasion cell number and wound healing rate were similar to those in the model group. RT-qPCR and WB assays showed that quercetin decreased miR-155 gene expression and p-JunB and p-FosB protein expression levels. Quercetin pretreatment can protect extravillous trophoblast cells from apoptosis and loss of migratory and invasive abilities through down-regulating the expression of miR-155, which may be a mechanism that inhibits the development of preeclampsia by in vitro study. |
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Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillousQuercetinPreeclampsiatrophoblastmicroRNAscell movementAbstract To investigate the effects of quercetin on the expression of microRNA-155 (miR-155) and the functions of lipopolysaccharide (LPS)-treated extravillous trophoblast cells. Cell apoptosis significantly increased and invasion cell number and wound healing rate significantly decreased in the model group compared with the NC group (P < 0.001). Quercetin supplement significantly improved the cell apoptosis rate, invasion cell number and wound healing rate (P < 0.05, respectively) compared with the model group. However, after miR-155 transfection, the cell apoptosis rate, invasion cell number and wound healing rate were similar to those in the model group. RT-qPCR and WB assays showed that quercetin decreased miR-155 gene expression and p-JunB and p-FosB protein expression levels. Quercetin pretreatment can protect extravillous trophoblast cells from apoptosis and loss of migratory and invasive abilities through down-regulating the expression of miR-155, which may be a mechanism that inhibits the development of preeclampsia by in vitro study.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101242Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.92221info:eu-repo/semantics/openAccessLIU,ChunhuaLIU,XianghongYANG,NingjuanWANG,Qingeng2022-07-11T00:00:00Zoai:scielo:S0101-20612022000101242Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-07-11T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous |
title |
Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous |
spellingShingle |
Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous LIU,Chunhua Quercetin Preeclampsia trophoblast microRNAs cell movement |
title_short |
Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous |
title_full |
Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous |
title_fullStr |
Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous |
title_full_unstemmed |
Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous |
title_sort |
Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous |
author |
LIU,Chunhua |
author_facet |
LIU,Chunhua LIU,Xianghong YANG,Ningjuan WANG,Qing |
author_role |
author |
author2 |
LIU,Xianghong YANG,Ningjuan WANG,Qing |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
LIU,Chunhua LIU,Xianghong YANG,Ningjuan WANG,Qing |
dc.subject.por.fl_str_mv |
Quercetin Preeclampsia trophoblast microRNAs cell movement |
topic |
Quercetin Preeclampsia trophoblast microRNAs cell movement |
description |
Abstract To investigate the effects of quercetin on the expression of microRNA-155 (miR-155) and the functions of lipopolysaccharide (LPS)-treated extravillous trophoblast cells. Cell apoptosis significantly increased and invasion cell number and wound healing rate significantly decreased in the model group compared with the NC group (P < 0.001). Quercetin supplement significantly improved the cell apoptosis rate, invasion cell number and wound healing rate (P < 0.05, respectively) compared with the model group. However, after miR-155 transfection, the cell apoptosis rate, invasion cell number and wound healing rate were similar to those in the model group. RT-qPCR and WB assays showed that quercetin decreased miR-155 gene expression and p-JunB and p-FosB protein expression levels. Quercetin pretreatment can protect extravillous trophoblast cells from apoptosis and loss of migratory and invasive abilities through down-regulating the expression of miR-155, which may be a mechanism that inhibits the development of preeclampsia by in vitro study. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101242 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101242 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.92221 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.42 2022 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126334997364736 |