Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous

Detalhes bibliográficos
Autor(a) principal: LIU,Chunhua
Data de Publicação: 2022
Outros Autores: LIU,Xianghong, YANG,Ningjuan, WANG,Qing
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101242
Resumo: Abstract To investigate the effects of quercetin on the expression of microRNA-155 (miR-155) and the functions of lipopolysaccharide (LPS)-treated extravillous trophoblast cells. Cell apoptosis significantly increased and invasion cell number and wound healing rate significantly decreased in the model group compared with the NC group (P < 0.001). Quercetin supplement significantly improved the cell apoptosis rate, invasion cell number and wound healing rate (P < 0.05, respectively) compared with the model group. However, after miR-155 transfection, the cell apoptosis rate, invasion cell number and wound healing rate were similar to those in the model group. RT-qPCR and WB assays showed that quercetin decreased miR-155 gene expression and p-JunB and p-FosB protein expression levels. Quercetin pretreatment can protect extravillous trophoblast cells from apoptosis and loss of migratory and invasive abilities through down-regulating the expression of miR-155, which may be a mechanism that inhibits the development of preeclampsia by in vitro study.
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spelling Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillousQuercetinPreeclampsiatrophoblastmicroRNAscell movementAbstract To investigate the effects of quercetin on the expression of microRNA-155 (miR-155) and the functions of lipopolysaccharide (LPS)-treated extravillous trophoblast cells. Cell apoptosis significantly increased and invasion cell number and wound healing rate significantly decreased in the model group compared with the NC group (P < 0.001). Quercetin supplement significantly improved the cell apoptosis rate, invasion cell number and wound healing rate (P < 0.05, respectively) compared with the model group. However, after miR-155 transfection, the cell apoptosis rate, invasion cell number and wound healing rate were similar to those in the model group. RT-qPCR and WB assays showed that quercetin decreased miR-155 gene expression and p-JunB and p-FosB protein expression levels. Quercetin pretreatment can protect extravillous trophoblast cells from apoptosis and loss of migratory and invasive abilities through down-regulating the expression of miR-155, which may be a mechanism that inhibits the development of preeclampsia by in vitro study.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101242Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.92221info:eu-repo/semantics/openAccessLIU,ChunhuaLIU,XianghongYANG,NingjuanWANG,Qingeng2022-07-11T00:00:00Zoai:scielo:S0101-20612022000101242Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-07-11T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous
title Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous
spellingShingle Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous
LIU,Chunhua
Quercetin
Preeclampsia
trophoblast
microRNAs
cell movement
title_short Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous
title_full Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous
title_fullStr Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous
title_full_unstemmed Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous
title_sort Quercetin inhibits the expression of miRNA-155 and improves the functions of lipopolysaccharide-induced human extravillous
author LIU,Chunhua
author_facet LIU,Chunhua
LIU,Xianghong
YANG,Ningjuan
WANG,Qing
author_role author
author2 LIU,Xianghong
YANG,Ningjuan
WANG,Qing
author2_role author
author
author
dc.contributor.author.fl_str_mv LIU,Chunhua
LIU,Xianghong
YANG,Ningjuan
WANG,Qing
dc.subject.por.fl_str_mv Quercetin
Preeclampsia
trophoblast
microRNAs
cell movement
topic Quercetin
Preeclampsia
trophoblast
microRNAs
cell movement
description Abstract To investigate the effects of quercetin on the expression of microRNA-155 (miR-155) and the functions of lipopolysaccharide (LPS)-treated extravillous trophoblast cells. Cell apoptosis significantly increased and invasion cell number and wound healing rate significantly decreased in the model group compared with the NC group (P < 0.001). Quercetin supplement significantly improved the cell apoptosis rate, invasion cell number and wound healing rate (P < 0.05, respectively) compared with the model group. However, after miR-155 transfection, the cell apoptosis rate, invasion cell number and wound healing rate were similar to those in the model group. RT-qPCR and WB assays showed that quercetin decreased miR-155 gene expression and p-JunB and p-FosB protein expression levels. Quercetin pretreatment can protect extravillous trophoblast cells from apoptosis and loss of migratory and invasive abilities through down-regulating the expression of miR-155, which may be a mechanism that inhibits the development of preeclampsia by in vitro study.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101242
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101242
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.92221
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.42 2022
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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