Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway

Detalhes bibliográficos
Autor(a) principal: LIU,Qian
Data de Publicação: 2022
Outros Autores: LIU,Guangxuan, ZHU,Ying, CHAO,Yingyan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100594
Resumo: Abstract Objective The paper investigated the effect and molecular mechanism of Houttuynin on breast cancer in vitro, so as to provide clinical basis for the treatment of breast cancer with Houttuynin. Methods: Cell survival rate was measured by MTT assay. Cell apoptosis was detected by flow cytometry and AO/EB double staining method. The expressions of PI3K, AKT, and mTOR proteins and their phosphorylation levels were determined by WB experiment. Results: Houttuynin can significantly inhibit the growth of breast cancer cells and arrest cell cycle in the G2/M phase. Houttuynin induced apoptosis of breast cancer cells through two pathways, namely, mitochondria and death receptors. It was verified by WB experiment that Houttuynin could induce autophagy in breast cancer cells and reduce the expressions of PI3K, AKT, mTOR and other proteins. Conclusion: Houttuynin induced breast cancer cell apoptosis by inhibiting PI3K/AKT signaling pathway, thereby inhibiting the proliferation of breast cancer cells.
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spelling Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathwayhouttuyninPI3K/AKTapoptosisAbstract Objective The paper investigated the effect and molecular mechanism of Houttuynin on breast cancer in vitro, so as to provide clinical basis for the treatment of breast cancer with Houttuynin. Methods: Cell survival rate was measured by MTT assay. Cell apoptosis was detected by flow cytometry and AO/EB double staining method. The expressions of PI3K, AKT, and mTOR proteins and their phosphorylation levels were determined by WB experiment. Results: Houttuynin can significantly inhibit the growth of breast cancer cells and arrest cell cycle in the G2/M phase. Houttuynin induced apoptosis of breast cancer cells through two pathways, namely, mitochondria and death receptors. It was verified by WB experiment that Houttuynin could induce autophagy in breast cancer cells and reduce the expressions of PI3K, AKT, mTOR and other proteins. Conclusion: Houttuynin induced breast cancer cell apoptosis by inhibiting PI3K/AKT signaling pathway, thereby inhibiting the proliferation of breast cancer cells.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100594Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.18721info:eu-repo/semantics/openAccessLIU,QianLIU,GuangxuanZHU,YingCHAO,Yingyaneng2022-02-22T00:00:00Zoai:scielo:S0101-20612022000100594Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-02-22T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway
title Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway
spellingShingle Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway
LIU,Qian
houttuynin
PI3K/AKT
apoptosis
title_short Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway
title_full Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway
title_fullStr Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway
title_full_unstemmed Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway
title_sort Study on the mechanism of houttuynin inducing apoptosis of MCF-7 cells by inhibiting PI3K/AKT signaling pathway
author LIU,Qian
author_facet LIU,Qian
LIU,Guangxuan
ZHU,Ying
CHAO,Yingyan
author_role author
author2 LIU,Guangxuan
ZHU,Ying
CHAO,Yingyan
author2_role author
author
author
dc.contributor.author.fl_str_mv LIU,Qian
LIU,Guangxuan
ZHU,Ying
CHAO,Yingyan
dc.subject.por.fl_str_mv houttuynin
PI3K/AKT
apoptosis
topic houttuynin
PI3K/AKT
apoptosis
description Abstract Objective The paper investigated the effect and molecular mechanism of Houttuynin on breast cancer in vitro, so as to provide clinical basis for the treatment of breast cancer with Houttuynin. Methods: Cell survival rate was measured by MTT assay. Cell apoptosis was detected by flow cytometry and AO/EB double staining method. The expressions of PI3K, AKT, and mTOR proteins and their phosphorylation levels were determined by WB experiment. Results: Houttuynin can significantly inhibit the growth of breast cancer cells and arrest cell cycle in the G2/M phase. Houttuynin induced apoptosis of breast cancer cells through two pathways, namely, mitochondria and death receptors. It was verified by WB experiment that Houttuynin could induce autophagy in breast cancer cells and reduce the expressions of PI3K, AKT, mTOR and other proteins. Conclusion: Houttuynin induced breast cancer cell apoptosis by inhibiting PI3K/AKT signaling pathway, thereby inhibiting the proliferation of breast cancer cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100594
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100594
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.18721
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.42 2022
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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