Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700205 |
Resumo: | ABSTRACT Purpose To clarify the best protocol for performing remote ischemic conditioning and to minimize the consequences of ischemia and reperfusion syndrome in brain, the present study aimed to evaluate different time protocols and the relation of the organs and the antioxidant effects of this technique. Methods The rat’s left femoral artery was clamped with a microvascular clamp in times that ranged from 1 to 5 minutes, according to the corresponding group. After the cycles of remote ischemic conditioning and a reperfusion of 20 minutes, the brain and the left gastrocnemius were collected. The samples were used to measure glutathione peroxidase, glutathione reductase and catalase levels. Results In the gastrocnemius, the 4-minute protocol increased the catalase concentration compared to the 1-minute protocol, but the latter increased both glutathione peroxidase and glutathione reductase compared to the former. On the other hand, the brain demonstrated higher catalase and glutathione peroxidase in 5-minute group, and the 3-minute group reached higher values of glutathione reductase. Conclusions Remote ischemic conditioning increases brain antioxidant capacity in a time-dependent way, while muscle presents higher protection on 1-minute cycles and tends to decrease its defence with longer cycles of intermittent occlusions of the femoral artery. |
id |
SBDPC-1_2c86f059b223c4b4ab31554b93a42297 |
---|---|
oai_identifier_str |
oai:scielo:S0102-86502021000700205 |
network_acronym_str |
SBDPC-1 |
network_name_str |
Acta Cirúrgica Brasileira (Online) |
repository_id_str |
|
spelling |
Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanismIschemiaReperfusion InjuryIschemic PostconditioningStrokeAntioxidantsRatsABSTRACT Purpose To clarify the best protocol for performing remote ischemic conditioning and to minimize the consequences of ischemia and reperfusion syndrome in brain, the present study aimed to evaluate different time protocols and the relation of the organs and the antioxidant effects of this technique. Methods The rat’s left femoral artery was clamped with a microvascular clamp in times that ranged from 1 to 5 minutes, according to the corresponding group. After the cycles of remote ischemic conditioning and a reperfusion of 20 minutes, the brain and the left gastrocnemius were collected. The samples were used to measure glutathione peroxidase, glutathione reductase and catalase levels. Results In the gastrocnemius, the 4-minute protocol increased the catalase concentration compared to the 1-minute protocol, but the latter increased both glutathione peroxidase and glutathione reductase compared to the former. On the other hand, the brain demonstrated higher catalase and glutathione peroxidase in 5-minute group, and the 3-minute group reached higher values of glutathione reductase. Conclusions Remote ischemic conditioning increases brain antioxidant capacity in a time-dependent way, while muscle presents higher protection on 1-minute cycles and tends to decrease its defence with longer cycles of intermittent occlusions of the femoral artery.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700205Acta Cirúrgica Brasileira v.36 n.7 2021reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/acb360707info:eu-repo/semantics/openAccessMonteiro,Andrew MoraesCouteiro,Rodrigo ParacampoSilva,Dora Fonseca daTrindade Júnior,Sérgio CunhaSilva,Renata CunhaSousa,Luís Fernando Freitas deSantos,Deivid Ramos dosFreitas,Jofre Jacob da SilvaBrito,Marcus Vinícius Henriqueseng2021-09-01T00:00:00Zoai:scielo:S0102-86502021000700205Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2021-09-01T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism |
title |
Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism |
spellingShingle |
Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism Monteiro,Andrew Moraes Ischemia Reperfusion Injury Ischemic Postconditioning Stroke Antioxidants Rats |
title_short |
Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism |
title_full |
Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism |
title_fullStr |
Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism |
title_full_unstemmed |
Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism |
title_sort |
Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism |
author |
Monteiro,Andrew Moraes |
author_facet |
Monteiro,Andrew Moraes Couteiro,Rodrigo Paracampo Silva,Dora Fonseca da Trindade Júnior,Sérgio Cunha Silva,Renata Cunha Sousa,Luís Fernando Freitas de Santos,Deivid Ramos dos Freitas,Jofre Jacob da Silva Brito,Marcus Vinícius Henriques |
author_role |
author |
author2 |
Couteiro,Rodrigo Paracampo Silva,Dora Fonseca da Trindade Júnior,Sérgio Cunha Silva,Renata Cunha Sousa,Luís Fernando Freitas de Santos,Deivid Ramos dos Freitas,Jofre Jacob da Silva Brito,Marcus Vinícius Henriques |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Monteiro,Andrew Moraes Couteiro,Rodrigo Paracampo Silva,Dora Fonseca da Trindade Júnior,Sérgio Cunha Silva,Renata Cunha Sousa,Luís Fernando Freitas de Santos,Deivid Ramos dos Freitas,Jofre Jacob da Silva Brito,Marcus Vinícius Henriques |
dc.subject.por.fl_str_mv |
Ischemia Reperfusion Injury Ischemic Postconditioning Stroke Antioxidants Rats |
topic |
Ischemia Reperfusion Injury Ischemic Postconditioning Stroke Antioxidants Rats |
description |
ABSTRACT Purpose To clarify the best protocol for performing remote ischemic conditioning and to minimize the consequences of ischemia and reperfusion syndrome in brain, the present study aimed to evaluate different time protocols and the relation of the organs and the antioxidant effects of this technique. Methods The rat’s left femoral artery was clamped with a microvascular clamp in times that ranged from 1 to 5 minutes, according to the corresponding group. After the cycles of remote ischemic conditioning and a reperfusion of 20 minutes, the brain and the left gastrocnemius were collected. The samples were used to measure glutathione peroxidase, glutathione reductase and catalase levels. Results In the gastrocnemius, the 4-minute protocol increased the catalase concentration compared to the 1-minute protocol, but the latter increased both glutathione peroxidase and glutathione reductase compared to the former. On the other hand, the brain demonstrated higher catalase and glutathione peroxidase in 5-minute group, and the 3-minute group reached higher values of glutathione reductase. Conclusions Remote ischemic conditioning increases brain antioxidant capacity in a time-dependent way, while muscle presents higher protection on 1-minute cycles and tends to decrease its defence with longer cycles of intermittent occlusions of the femoral artery. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700205 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700205 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/acb360707 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.36 n.7 2021 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126446268055552 |