Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

• Autor(a) principal: Dornelas,Conceição Aparecida
• Data de Publicação: 2012
• Outros Autores: Fechine-Jamacaru,Francisco Vagnaldo, Albuquerque,Irineu Lima, Magalhães,Hemerson Iury Ferreira, Dias,Thiago Alves, Faria,Mário Henrique Girão, Alves,Markênia Kely Santos, Rabenhorst,Silvia Helena Barem, Almeida,Paulo Roberto Carvalho de, Lemos,Telma Leda Gomes de, Castro,José Daniel Vieira de, Moraes,Maria Elisabete Amaral, Moraes,Manoel Odorico
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000800003
• topic: Lysine; Propolis; Angiogenesis Inhibitors; Urogenital Neoplasms; Rats

PURPOSE: To determine the effects of water-soluble derivative of green propolis in bladder cancer angiogenesis in rats given N-butyl-(-4-hydroxybutyl) nitrosamine (BBN). METHODS: Nine groups were established, where six of them (Groups 1 to 6), the animals received 0.05% BBN in their drinking water for 14 weeks. From the 32nd to the 40th week, Groups 1, 2, 3 and 4 were treated respectively with water, L-lysine (300 mg/kg/day), celecoxib (30 mg/kg/day) and propolis (300 mg/kg/day). Groups 5 and 6 were given propolis and L-lysine from the 1st to the 40th week (150 mg/kg/day). Microvascular density was determined by histological sections stained for the marker CD-31 and analyzed with specific software. RESULTS: The microvascular density in bladder carcinomas was lower (p<0.01) in rats receiving propolis than in controls given carcinogen only. On the other hand, the microvascular density of tumors in rats receiving carcinogen and L-lysine for 40 weeks from the beginning of carcinogen treatment was significantly higher (p<0.01) than in the corresponding controls. CONCLUSION: Water-soluble derivative of propolis inhibits angiogenesis in BBN-induced rat bladder cancer, while L-lysine treatment stimulates angiogenesis if initiated concurrently with BBN.