Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistance
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de entomologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262021000100206 |
Resumo: | Abstract Londrina is the fourth most populous city in southern Brazil. Its subtropical weather with rain in all seasons, as well as its high population density, make the city perfect for the Aedes aegypti (Linnaeus, 1762) life cycle. Over the last few years, Londrina presented high infestation indexes and was one of the cities with the most reported cases of dengue. Uncontrolled use of synthetic insecticides may influence the mosquito’s genetic composition. In this paper, we studied mitochondrial DNA and kdr mutations in Aedes aegypti. The analysis of the ND4 gene in 330 specimens showed the presence of 27 haplotypes. The pyrethroid resistance alleles (kdr) evaluated are present in the collected populations, with a 50% frequency of the Val1016Ile and 48% of the Phe1534Cys mutations. Such analysis of the mutations in the populations collected at the State University of Londrina’s campus – a microenvironment that differs from the rest of the city – showed frequencies of 57% and 62%, respectively. The low gene flow observed, Nm = 0.11 and Nm = 0.10, along with the elevated differentiation, Fst = 0.19 and Fst = 0.18, among populations suggest an influence of genetic drift. The strong presence of resistance alleles kdr in the city is evident, which demonstrates that even with the interruption of the use of pyrethroids by the National Dengue Control Program, resistance may be maintained due to domestic use. Thus, the results have shown the need for genetic monitoring, alongside other entomological surveillance monitoring tools, to create strategies of mosquito control. |
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Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistanceallele-specific PCRknockdown resistancemtDNAND4 genevoltage-gated sodium channelAbstract Londrina is the fourth most populous city in southern Brazil. Its subtropical weather with rain in all seasons, as well as its high population density, make the city perfect for the Aedes aegypti (Linnaeus, 1762) life cycle. Over the last few years, Londrina presented high infestation indexes and was one of the cities with the most reported cases of dengue. Uncontrolled use of synthetic insecticides may influence the mosquito’s genetic composition. In this paper, we studied mitochondrial DNA and kdr mutations in Aedes aegypti. The analysis of the ND4 gene in 330 specimens showed the presence of 27 haplotypes. The pyrethroid resistance alleles (kdr) evaluated are present in the collected populations, with a 50% frequency of the Val1016Ile and 48% of the Phe1534Cys mutations. Such analysis of the mutations in the populations collected at the State University of Londrina’s campus – a microenvironment that differs from the rest of the city – showed frequencies of 57% and 62%, respectively. The low gene flow observed, Nm = 0.11 and Nm = 0.10, along with the elevated differentiation, Fst = 0.19 and Fst = 0.18, among populations suggest an influence of genetic drift. The strong presence of resistance alleles kdr in the city is evident, which demonstrates that even with the interruption of the use of pyrethroids by the National Dengue Control Program, resistance may be maintained due to domestic use. Thus, the results have shown the need for genetic monitoring, alongside other entomological surveillance monitoring tools, to create strategies of mosquito control.Sociedade Brasileira De Entomologia2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262021000100206Revista Brasileira de Entomologia v.65 n.1 2021reponame:Revista brasileira de entomologia (Online)instname:Sociedade Brasileira De Entomologia (SBE)instacron:SBE10.1590/1806-9665-rbent-2020-0088info:eu-repo/semantics/openAccessLopes,Thayná Bisson FerrazAmaro,Tafarel RibeiroSilva,Bianca PiracciniZequi,João Antonio CyrinoVilas-Bôas,Gislayne TrindadeSilva,Mario Antonio Navarro daVilas-Boas,Beatriz Trindadeda Rosa,Renataeng2021-03-02T00:00:00Zoai:scielo:S0085-56262021000100206Revistahttp://www.rbentomologia.com/https://old.scielo.br/oai/scielo-oai.php||sbe@ufpr.br1806-96650085-5626opendoar:2021-03-02T00:00Revista brasileira de entomologia (Online) - Sociedade Brasileira De Entomologia (SBE)false |
dc.title.none.fl_str_mv |
Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistance |
title |
Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistance |
spellingShingle |
Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistance Lopes,Thayná Bisson Ferraz allele-specific PCR knockdown resistance mtDNA ND4 gene voltage-gated sodium channel |
title_short |
Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistance |
title_full |
Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistance |
title_fullStr |
Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistance |
title_full_unstemmed |
Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistance |
title_sort |
Genetic study in Aedes (Stegomyia) aegypti (Linnaeus, 1762) from Londrina (Paraná State, Brazil): an approach to population structure and pyrethroid resistance |
author |
Lopes,Thayná Bisson Ferraz |
author_facet |
Lopes,Thayná Bisson Ferraz Amaro,Tafarel Ribeiro Silva,Bianca Piraccini Zequi,João Antonio Cyrino Vilas-Bôas,Gislayne Trindade Silva,Mario Antonio Navarro da Vilas-Boas,Beatriz Trindade da Rosa,Renata |
author_role |
author |
author2 |
Amaro,Tafarel Ribeiro Silva,Bianca Piraccini Zequi,João Antonio Cyrino Vilas-Bôas,Gislayne Trindade Silva,Mario Antonio Navarro da Vilas-Boas,Beatriz Trindade da Rosa,Renata |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Lopes,Thayná Bisson Ferraz Amaro,Tafarel Ribeiro Silva,Bianca Piraccini Zequi,João Antonio Cyrino Vilas-Bôas,Gislayne Trindade Silva,Mario Antonio Navarro da Vilas-Boas,Beatriz Trindade da Rosa,Renata |
dc.subject.por.fl_str_mv |
allele-specific PCR knockdown resistance mtDNA ND4 gene voltage-gated sodium channel |
topic |
allele-specific PCR knockdown resistance mtDNA ND4 gene voltage-gated sodium channel |
description |
Abstract Londrina is the fourth most populous city in southern Brazil. Its subtropical weather with rain in all seasons, as well as its high population density, make the city perfect for the Aedes aegypti (Linnaeus, 1762) life cycle. Over the last few years, Londrina presented high infestation indexes and was one of the cities with the most reported cases of dengue. Uncontrolled use of synthetic insecticides may influence the mosquito’s genetic composition. In this paper, we studied mitochondrial DNA and kdr mutations in Aedes aegypti. The analysis of the ND4 gene in 330 specimens showed the presence of 27 haplotypes. The pyrethroid resistance alleles (kdr) evaluated are present in the collected populations, with a 50% frequency of the Val1016Ile and 48% of the Phe1534Cys mutations. Such analysis of the mutations in the populations collected at the State University of Londrina’s campus – a microenvironment that differs from the rest of the city – showed frequencies of 57% and 62%, respectively. The low gene flow observed, Nm = 0.11 and Nm = 0.10, along with the elevated differentiation, Fst = 0.19 and Fst = 0.18, among populations suggest an influence of genetic drift. The strong presence of resistance alleles kdr in the city is evident, which demonstrates that even with the interruption of the use of pyrethroids by the National Dengue Control Program, resistance may be maintained due to domestic use. Thus, the results have shown the need for genetic monitoring, alongside other entomological surveillance monitoring tools, to create strategies of mosquito control. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262021000100206 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262021000100206 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1806-9665-rbent-2020-0088 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira De Entomologia |
publisher.none.fl_str_mv |
Sociedade Brasileira De Entomologia |
dc.source.none.fl_str_mv |
Revista Brasileira de Entomologia v.65 n.1 2021 reponame:Revista brasileira de entomologia (Online) instname:Sociedade Brasileira De Entomologia (SBE) instacron:SBE |
instname_str |
Sociedade Brasileira De Entomologia (SBE) |
instacron_str |
SBE |
institution |
SBE |
reponame_str |
Revista brasileira de entomologia (Online) |
collection |
Revista brasileira de entomologia (Online) |
repository.name.fl_str_mv |
Revista brasileira de entomologia (Online) - Sociedade Brasileira De Entomologia (SBE) |
repository.mail.fl_str_mv |
||sbe@ufpr.br |
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1752126461484990464 |