Analysis of the nucleotide sequence of the coat protein and 3'-untranslated region of two Brazilian Potato virus Y isolates

• Autor(a) principal: INOUE-NAGATA,ALICE K.
• Data de Publicação: 2001
• Outros Autores: FONSECA,M. ESTHER N., LOBO,TATIANA O.T.A., ÁVILA,ANTÔNIO C. DE, MONTE,DAMARES C.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Fitopatologia Brasileira
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000100008
• topic: PVY; Potyvirus; phylogenetic analysis; taxonomy; coat protein

Two Brazilian Potato virus Y (PVY) isolates were biologically characterized as necrotic (PVY-NBR) and common (PVY-OBR) based upon symptoms on test plants. Additional characterization was performed by sequencing a cDNA corresponding to the 3' terminal region of the viral genome. The sequence consisted of 195 nucleotides (nt) coding part of the nuclear inclusion body b (NIb) gene, 804 nt of the coat protein (CP) gene, and 328 nt (PVY-OBR) or 326 nt (PVY-NBR) of the 3'-untranslated region (UTR). Translation of the sequence resulted in one single open reading frame with part of the NIb and a CP of 267 amino acids. The two isolates shared 95.1% similarity in the CP amino acid sequence. The CP and the 3'-UTR sequence of the Brazilian isolates were compared to those of other PVY isolates previously reported and unrooted phylogenetic trees were constructed. The trees revealed a separation of two distinct clusters, one comprising most of the common strains and the other comprising the necrotic strains. PVY-OBR was clustered in the common group and PVY-NBR in the necrotic one.