Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Fitopatologia Brasileira |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007 |
Resumo: | Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were compared with related viruses reported in different geographical regions of the world. The analysis showed closed relations considering the Brazilian isolates of OYDV-G and GCLV, and large divergence considering LYSV isolate. The detection of these virus species was confirmed by specific reactions observed when coat protein genes of the Brazilian isolates were used as probes in dot-blot and Southern blot hybridization assays. In field natural viral re-infection of virus-free garlic was evaluated. |
id |
SBF-3_42602aabc8aa4e6325dfb88382f841b3 |
---|---|
oai_identifier_str |
oai:scielo:S0100-41582001000300007 |
network_acronym_str |
SBF-3 |
network_name_str |
Fitopatologia Brasileira |
repository_id_str |
|
spelling |
Garlic viral complex: identification of Potyviruses and Carlavirus in Central BrazilOYDV-GLYSVGCLVdetectioncoat proteinPCRprobesepidemiologyGarlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were compared with related viruses reported in different geographical regions of the world. The analysis showed closed relations considering the Brazilian isolates of OYDV-G and GCLV, and large divergence considering LYSV isolate. The detection of these virus species was confirmed by specific reactions observed when coat protein genes of the Brazilian isolates were used as probes in dot-blot and Southern blot hybridization assays. In field natural viral re-infection of virus-free garlic was evaluated.Sociedade Brasileira de Fitopatologia2001-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007Fitopatologia Brasileira v.26 n.3 2001reponame:Fitopatologia Brasileirainstname:Sociedade Brasileira de Fitopatologia (SBF)instacron:SBF10.1590/S0100-41582001000300007info:eu-repo/semantics/openAccessFAJARDO,THOR V. M.NISHIJIMA,MARTABUSO,JOSÉ A.TORRES,ANTÔNIO C.ÁVILA,ANTÔNIO C.RESENDE,RENATO O.eng2001-11-28T00:00:00Zoai:scielo:S0100-41582001000300007Revistahttp://www.scielo.br/fbONGhttps://old.scielo.br/oai/scielo-oai.php||sbf-revista@ufla.br1678-46770100-4158opendoar:2001-11-28T00:00Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)false |
dc.title.none.fl_str_mv |
Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil |
title |
Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil |
spellingShingle |
Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil FAJARDO,THOR V. M. OYDV-G LYSV GCLV detection coat protein PCR probes epidemiology |
title_short |
Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil |
title_full |
Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil |
title_fullStr |
Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil |
title_full_unstemmed |
Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil |
title_sort |
Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil |
author |
FAJARDO,THOR V. M. |
author_facet |
FAJARDO,THOR V. M. NISHIJIMA,MARTA BUSO,JOSÉ A. TORRES,ANTÔNIO C. ÁVILA,ANTÔNIO C. RESENDE,RENATO O. |
author_role |
author |
author2 |
NISHIJIMA,MARTA BUSO,JOSÉ A. TORRES,ANTÔNIO C. ÁVILA,ANTÔNIO C. RESENDE,RENATO O. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
FAJARDO,THOR V. M. NISHIJIMA,MARTA BUSO,JOSÉ A. TORRES,ANTÔNIO C. ÁVILA,ANTÔNIO C. RESENDE,RENATO O. |
dc.subject.por.fl_str_mv |
OYDV-G LYSV GCLV detection coat protein PCR probes epidemiology |
topic |
OYDV-G LYSV GCLV detection coat protein PCR probes epidemiology |
description |
Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were compared with related viruses reported in different geographical regions of the world. The analysis showed closed relations considering the Brazilian isolates of OYDV-G and GCLV, and large divergence considering LYSV isolate. The detection of these virus species was confirmed by specific reactions observed when coat protein genes of the Brazilian isolates were used as probes in dot-blot and Southern blot hybridization assays. In field natural viral re-infection of virus-free garlic was evaluated. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-41582001000300007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Fitopatologia Brasileira v.26 n.3 2001 reponame:Fitopatologia Brasileira instname:Sociedade Brasileira de Fitopatologia (SBF) instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia (SBF) |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Fitopatologia Brasileira |
collection |
Fitopatologia Brasileira |
repository.name.fl_str_mv |
Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF) |
repository.mail.fl_str_mv |
||sbf-revista@ufla.br |
_version_ |
1754734648567529472 |