Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000500003 |
Resumo: | Epicoccum sp. showed in vitro antagonism to the rice pathogens Magnaporthe oryzae, Rhizoctonia solani, Sarocladium oryzae, Monographella albescens and Cochliobolus miyabeanus in dual culture. The colony growth of the rice blast fungus, M. oryzae, was reduced by 42.50%. The lethal doses (LD50) determined based on probit-log dosage response curves at 3 and 6 hours after germination were 393.0 and 326.6 ppm, respectively. The crude extract (600 ppm) reduced appressorial formation by 95.68%. A greenhouse experiment comparing the relative efficiency of conidial suspension and crude extract of Epicoccum sp. in the suppression of leaf blast showed no statistical difference between both application methods. However, the crude extract of Epicoccum sp. (4000 ppm) 48 hours before the application of M. oryzae induced resistance and suppressed leaf blast by 97.6%. Scanning electron microscopy of rice leaves inoculated with crude extract of Epicoccum sp. and challenged with M. oryzae showed appressorial deformation on penetration phase. Peroxidase and β-1,3-glucanase activities increased in plants sprayed with crude extract of Epicoccum sp., 24 hours after the application of the challenger. PAL as well as chitinase activities increased 72 hours after challenge inoculation. Epicoccum sp. was shown to be a potential antagonist and inducer of resistance against M. oryzae. |
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Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum spEpicoccum sp.Magnaporthe oryzaeOryza sativaantagonismISRPR proteinsEpicoccum sp. showed in vitro antagonism to the rice pathogens Magnaporthe oryzae, Rhizoctonia solani, Sarocladium oryzae, Monographella albescens and Cochliobolus miyabeanus in dual culture. The colony growth of the rice blast fungus, M. oryzae, was reduced by 42.50%. The lethal doses (LD50) determined based on probit-log dosage response curves at 3 and 6 hours after germination were 393.0 and 326.6 ppm, respectively. The crude extract (600 ppm) reduced appressorial formation by 95.68%. A greenhouse experiment comparing the relative efficiency of conidial suspension and crude extract of Epicoccum sp. in the suppression of leaf blast showed no statistical difference between both application methods. However, the crude extract of Epicoccum sp. (4000 ppm) 48 hours before the application of M. oryzae induced resistance and suppressed leaf blast by 97.6%. Scanning electron microscopy of rice leaves inoculated with crude extract of Epicoccum sp. and challenged with M. oryzae showed appressorial deformation on penetration phase. Peroxidase and β-1,3-glucanase activities increased in plants sprayed with crude extract of Epicoccum sp., 24 hours after the application of the challenger. PAL as well as chitinase activities increased 72 hours after challenge inoculation. Epicoccum sp. was shown to be a potential antagonist and inducer of resistance against M. oryzae.Sociedade Brasileira de Fitopatologia2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000500003Tropical Plant Pathology v.38 n.5 2013reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762013005000028info:eu-repo/semantics/openAccessSena,Ana P.A.Chaibub,Amanda A.Côrtes,Márcio V.C.B.Silva,Gisele B.Silva-Lobo,Valácia L.Prabhu,Anne S.Filippi,Marta C.C.Araújo,Leila G.eng2013-10-29T00:00:00Zoai:scielo:S1982-56762013000500003Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2013-10-29T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp |
title |
Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp |
spellingShingle |
Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp Sena,Ana P.A. Epicoccum sp. Magnaporthe oryzae Oryza sativa antagonism ISR PR proteins |
title_short |
Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp |
title_full |
Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp |
title_fullStr |
Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp |
title_full_unstemmed |
Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp |
title_sort |
Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp |
author |
Sena,Ana P.A. |
author_facet |
Sena,Ana P.A. Chaibub,Amanda A. Côrtes,Márcio V.C.B. Silva,Gisele B. Silva-Lobo,Valácia L. Prabhu,Anne S. Filippi,Marta C.C. Araújo,Leila G. |
author_role |
author |
author2 |
Chaibub,Amanda A. Côrtes,Márcio V.C.B. Silva,Gisele B. Silva-Lobo,Valácia L. Prabhu,Anne S. Filippi,Marta C.C. Araújo,Leila G. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Sena,Ana P.A. Chaibub,Amanda A. Côrtes,Márcio V.C.B. Silva,Gisele B. Silva-Lobo,Valácia L. Prabhu,Anne S. Filippi,Marta C.C. Araújo,Leila G. |
dc.subject.por.fl_str_mv |
Epicoccum sp. Magnaporthe oryzae Oryza sativa antagonism ISR PR proteins |
topic |
Epicoccum sp. Magnaporthe oryzae Oryza sativa antagonism ISR PR proteins |
description |
Epicoccum sp. showed in vitro antagonism to the rice pathogens Magnaporthe oryzae, Rhizoctonia solani, Sarocladium oryzae, Monographella albescens and Cochliobolus miyabeanus in dual culture. The colony growth of the rice blast fungus, M. oryzae, was reduced by 42.50%. The lethal doses (LD50) determined based on probit-log dosage response curves at 3 and 6 hours after germination were 393.0 and 326.6 ppm, respectively. The crude extract (600 ppm) reduced appressorial formation by 95.68%. A greenhouse experiment comparing the relative efficiency of conidial suspension and crude extract of Epicoccum sp. in the suppression of leaf blast showed no statistical difference between both application methods. However, the crude extract of Epicoccum sp. (4000 ppm) 48 hours before the application of M. oryzae induced resistance and suppressed leaf blast by 97.6%. Scanning electron microscopy of rice leaves inoculated with crude extract of Epicoccum sp. and challenged with M. oryzae showed appressorial deformation on penetration phase. Peroxidase and β-1,3-glucanase activities increased in plants sprayed with crude extract of Epicoccum sp., 24 hours after the application of the challenger. PAL as well as chitinase activities increased 72 hours after challenge inoculation. Epicoccum sp. was shown to be a potential antagonist and inducer of resistance against M. oryzae. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000500003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000500003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762013005000028 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.38 n.5 2013 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824586335092736 |