Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2006000100005 |
Resumo: | The cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis (HEXC) were studied in rats using a combined in vivo and in vitro approach. In non-anesthetized rats, HEXC injections produced a significant and dose-dependent hypotension associated with an increase in heart rate. The hypotensive response was not attenuated after nitric oxide (NO) synthase blockade, L-NAME (20 mg/Kg, i.v.). In isolated rat superior aortic rings, HEXC was able to relax the tonus induced by phenylephrine (1 µM) and KCl (80 mM), (EC50 = 85±13 and 62±5 µg/mL, respectively). The smooth muscle-relaxant activity of HEXC was not inhibited by removal of vascular endothelium (EC50 = 58±6 µg/mL). HEXC antagonized CaCl2-induced contractions in depolarizing medium nominally without Ca2+. HEXC inhibited the intracellular calcium-dependent transient contractions induced by caffeine (20 mM) in Ca2+-free solution, but not those induced by norepinephrine (1 µM). In isolated rat atrial preparations, HEXC produced negative inotropic and chronotropic responses (IC50= 534±42 and 259±22 µg/mL, respectively). The results obtained suggest that the hypotensive effect of HEXC is probably due to a peripheral vasodilatation, at least, secondary to an interference with the Ca2+ mobilization as a consequence of voltage-dependent Ca2+ channel blockade and the inhibition of Ca2+ release from caffeine-sensitive intracellular stores. Finally, HEXC acts directly on the heart decreasing contractility and heart rate, these effects are of little importance to the expression of the hypotensive response induced by HEXC. |
id |
SBFGNOSIA-1_0800dee284f86a99684dcc404708941a |
---|---|
oai_identifier_str |
oai:scielo:S0102-695X2006000100005 |
network_acronym_str |
SBFGNOSIA-1 |
network_name_str |
Revista Brasileira de Farmacognosia (Online) |
repository_id_str |
|
spelling |
Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in ratsXylopia cayennensisAnnonaceaecardiovascular effectsisolated atriaisolated rat aortic ringsCa2+ influxThe cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis (HEXC) were studied in rats using a combined in vivo and in vitro approach. In non-anesthetized rats, HEXC injections produced a significant and dose-dependent hypotension associated with an increase in heart rate. The hypotensive response was not attenuated after nitric oxide (NO) synthase blockade, L-NAME (20 mg/Kg, i.v.). In isolated rat superior aortic rings, HEXC was able to relax the tonus induced by phenylephrine (1 µM) and KCl (80 mM), (EC50 = 85±13 and 62±5 µg/mL, respectively). The smooth muscle-relaxant activity of HEXC was not inhibited by removal of vascular endothelium (EC50 = 58±6 µg/mL). HEXC antagonized CaCl2-induced contractions in depolarizing medium nominally without Ca2+. HEXC inhibited the intracellular calcium-dependent transient contractions induced by caffeine (20 mM) in Ca2+-free solution, but not those induced by norepinephrine (1 µM). In isolated rat atrial preparations, HEXC produced negative inotropic and chronotropic responses (IC50= 534±42 and 259±22 µg/mL, respectively). The results obtained suggest that the hypotensive effect of HEXC is probably due to a peripheral vasodilatation, at least, secondary to an interference with the Ca2+ mobilization as a consequence of voltage-dependent Ca2+ channel blockade and the inhibition of Ca2+ release from caffeine-sensitive intracellular stores. Finally, HEXC acts directly on the heart decreasing contractility and heart rate, these effects are of little importance to the expression of the hypotensive response induced by HEXC.Sociedade Brasileira de Farmacognosia2006-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2006000100005Revista Brasileira de Farmacognosia v.16 n.1 2006reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2006000100005info:eu-repo/semantics/openAccessNascimento,Alessandra A.Ribeiro,Êurica A. NogueiraOliveira,Joelisia M.Medeiros,Fernando A.Silva,Marcelo S.Medeiros,Isac A.eng2008-02-26T00:00:00Zoai:scielo:S0102-695X2006000100005Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2008-02-26T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in rats |
title |
Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in rats |
spellingShingle |
Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in rats Nascimento,Alessandra A. Xylopia cayennensis Annonaceae cardiovascular effects isolated atria isolated rat aortic rings Ca2+ influx |
title_short |
Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in rats |
title_full |
Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in rats |
title_fullStr |
Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in rats |
title_full_unstemmed |
Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in rats |
title_sort |
Cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis in rats |
author |
Nascimento,Alessandra A. |
author_facet |
Nascimento,Alessandra A. Ribeiro,Êurica A. Nogueira Oliveira,Joelisia M. Medeiros,Fernando A. Silva,Marcelo S. Medeiros,Isac A. |
author_role |
author |
author2 |
Ribeiro,Êurica A. Nogueira Oliveira,Joelisia M. Medeiros,Fernando A. Silva,Marcelo S. Medeiros,Isac A. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento,Alessandra A. Ribeiro,Êurica A. Nogueira Oliveira,Joelisia M. Medeiros,Fernando A. Silva,Marcelo S. Medeiros,Isac A. |
dc.subject.por.fl_str_mv |
Xylopia cayennensis Annonaceae cardiovascular effects isolated atria isolated rat aortic rings Ca2+ influx |
topic |
Xylopia cayennensis Annonaceae cardiovascular effects isolated atria isolated rat aortic rings Ca2+ influx |
description |
The cardiovascular effects induced by the hydroalcoholic extract of the stem of Xylopia cayennensis (HEXC) were studied in rats using a combined in vivo and in vitro approach. In non-anesthetized rats, HEXC injections produced a significant and dose-dependent hypotension associated with an increase in heart rate. The hypotensive response was not attenuated after nitric oxide (NO) synthase blockade, L-NAME (20 mg/Kg, i.v.). In isolated rat superior aortic rings, HEXC was able to relax the tonus induced by phenylephrine (1 µM) and KCl (80 mM), (EC50 = 85±13 and 62±5 µg/mL, respectively). The smooth muscle-relaxant activity of HEXC was not inhibited by removal of vascular endothelium (EC50 = 58±6 µg/mL). HEXC antagonized CaCl2-induced contractions in depolarizing medium nominally without Ca2+. HEXC inhibited the intracellular calcium-dependent transient contractions induced by caffeine (20 mM) in Ca2+-free solution, but not those induced by norepinephrine (1 µM). In isolated rat atrial preparations, HEXC produced negative inotropic and chronotropic responses (IC50= 534±42 and 259±22 µg/mL, respectively). The results obtained suggest that the hypotensive effect of HEXC is probably due to a peripheral vasodilatation, at least, secondary to an interference with the Ca2+ mobilization as a consequence of voltage-dependent Ca2+ channel blockade and the inhibition of Ca2+ release from caffeine-sensitive intracellular stores. Finally, HEXC acts directly on the heart decreasing contractility and heart rate, these effects are of little importance to the expression of the hypotensive response induced by HEXC. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2006000100005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2006000100005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-695X2006000100005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.16 n.1 2006 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122462091345920 |