Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins)
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000500018 |
Resumo: | Considering the traditional use of Casearia sylvestris Sw., Salicaceae, to threat gastric injuries and the pre-clinical studies showing its efficacy we aimed to screen other species to explore the biological activity of some species of this family. For this, we used a protease inhibition assay as a model for searching gastric anti-ulcer plant extracts. The ethanolic and aqueous extracts from branches and leafs of C. gossypiosperma, C. decandra and C. rupestris showed high percentage inhibition of pepsin, approximately 50%, with 1 μg/mL concentration. Curiously, C. obliquoa and Flacourtia ramontchi did not inhibit pepsin, but its most apolar extract showed inhibitory activity in the subtilisin assay. The enriched fraction of clerodane diterpenes inhibited the activity (42.75%) of pepsin with 1 ug/mL, but it did not inhibit subtilisin (23.76%). The results obtained with apolar and polar extracts from branches and leaves of some species of Salicaceae showed a different pattern of inhibition of two proteases, the aspartic pepsin and the serinic subtilisin, related with different biological activities. The results with the enriched fraction of clerodane diterpenes suggests that the activity observed with the C. sylvestris may be related with the presence of these substances in the crude extract. |
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Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins)SalicaceaeCaseariaclerodane diterpenesgastric anti-ulcer activityprotease inhibitionfluorescence assayConsidering the traditional use of Casearia sylvestris Sw., Salicaceae, to threat gastric injuries and the pre-clinical studies showing its efficacy we aimed to screen other species to explore the biological activity of some species of this family. For this, we used a protease inhibition assay as a model for searching gastric anti-ulcer plant extracts. The ethanolic and aqueous extracts from branches and leafs of C. gossypiosperma, C. decandra and C. rupestris showed high percentage inhibition of pepsin, approximately 50%, with 1 μg/mL concentration. Curiously, C. obliquoa and Flacourtia ramontchi did not inhibit pepsin, but its most apolar extract showed inhibitory activity in the subtilisin assay. The enriched fraction of clerodane diterpenes inhibited the activity (42.75%) of pepsin with 1 ug/mL, but it did not inhibit subtilisin (23.76%). The results obtained with apolar and polar extracts from branches and leaves of some species of Salicaceae showed a different pattern of inhibition of two proteases, the aspartic pepsin and the serinic subtilisin, related with different biological activities. The results with the enriched fraction of clerodane diterpenes suggests that the activity observed with the C. sylvestris may be related with the presence of these substances in the crude extract.Sociedade Brasileira de Farmacognosia2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000500018Revista Brasileira de Farmacognosia v.19 n.3 2009reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2009000500018info:eu-repo/semantics/openAccessFlausino Jr,OtavioAbissi,Barbara M.Vieira Jr,Gerardo MagelaSantos,André Gonzaga dosSilva,Dulce Helena daCavalheiro,AlbertoBolzani,Vanderlan da Silvaeng2010-01-22T00:00:00Zoai:scielo:S0102-695X2009000500018Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2010-01-22T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins) |
title |
Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins) |
spellingShingle |
Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins) Flausino Jr,Otavio Salicaceae Casearia clerodane diterpenes gastric anti-ulcer activity protease inhibition fluorescence assay |
title_short |
Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins) |
title_full |
Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins) |
title_fullStr |
Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins) |
title_full_unstemmed |
Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins) |
title_sort |
Protease inhibition activity of extracts from Salicaceae species from Brazilian Cerrado and Atlantic Rain Forest and of an enriched fraction of clerodane diterpenes (casearins) |
author |
Flausino Jr,Otavio |
author_facet |
Flausino Jr,Otavio Abissi,Barbara M. Vieira Jr,Gerardo Magela Santos,André Gonzaga dos Silva,Dulce Helena da Cavalheiro,Alberto Bolzani,Vanderlan da Silva |
author_role |
author |
author2 |
Abissi,Barbara M. Vieira Jr,Gerardo Magela Santos,André Gonzaga dos Silva,Dulce Helena da Cavalheiro,Alberto Bolzani,Vanderlan da Silva |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Flausino Jr,Otavio Abissi,Barbara M. Vieira Jr,Gerardo Magela Santos,André Gonzaga dos Silva,Dulce Helena da Cavalheiro,Alberto Bolzani,Vanderlan da Silva |
dc.subject.por.fl_str_mv |
Salicaceae Casearia clerodane diterpenes gastric anti-ulcer activity protease inhibition fluorescence assay |
topic |
Salicaceae Casearia clerodane diterpenes gastric anti-ulcer activity protease inhibition fluorescence assay |
description |
Considering the traditional use of Casearia sylvestris Sw., Salicaceae, to threat gastric injuries and the pre-clinical studies showing its efficacy we aimed to screen other species to explore the biological activity of some species of this family. For this, we used a protease inhibition assay as a model for searching gastric anti-ulcer plant extracts. The ethanolic and aqueous extracts from branches and leafs of C. gossypiosperma, C. decandra and C. rupestris showed high percentage inhibition of pepsin, approximately 50%, with 1 μg/mL concentration. Curiously, C. obliquoa and Flacourtia ramontchi did not inhibit pepsin, but its most apolar extract showed inhibitory activity in the subtilisin assay. The enriched fraction of clerodane diterpenes inhibited the activity (42.75%) of pepsin with 1 ug/mL, but it did not inhibit subtilisin (23.76%). The results obtained with apolar and polar extracts from branches and leaves of some species of Salicaceae showed a different pattern of inhibition of two proteases, the aspartic pepsin and the serinic subtilisin, related with different biological activities. The results with the enriched fraction of clerodane diterpenes suggests that the activity observed with the C. sylvestris may be related with the presence of these substances in the crude extract. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000500018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000500018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-695X2009000500018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.19 n.3 2009 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122463840370688 |