Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profile

Detalhes bibliográficos
Autor(a) principal: Santos,Vanessa N. C.
Data de Publicação: 2009
Outros Autores: Freitas,Rilton A. de, Deschamps,Francisco C., Biavatti,Maique W.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000300004
Resumo: Bromelia antiacantha (known as wild banana) is traditionally used to treat coughs, as an emollient and antitussive, and to treat mouth and skin ulcers. To investigate the radical scavenger properties, DPPH and phosphomolybdenium were used, with low antioxidant activity being observed for both (below 5 mg/ml of extract presented no activity for both techniques). An HPLC fingerprint of phenolic compounds was developed and total phenolics through Folin-Ciocalteau method was an average of 500 µg/g of fresh fruits. Any cytotoxicity was observed on L929 fibroblasts cells for aqueous, methanol and lipid extracts between 500 and 0.01 µg/ ml. The wild banana extract (1000 µg/mL) shown a lower genotoxicity when compared to the negative and positive (MMS) controls. Abundant calcium oxalate crystals and sucrose were found and characterized through NMR and X-ray diffraction respectively. Its centesimal composition indicated abundant carbohydrates (45%) and lipids (18%). The carbohydrate analysis showed acidic monosaccharides, and the lipid analysis shown palmitic and linoleic acids in similar quantities (30% each) and oleic acid 20%, among others, through GC-FID. The viscosity of the water-soluble polysaccharide sub-fraction (5 g/L) was similar to that of water.
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spelling Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profileBromelia antiacanthaantioxidantcytotoxicitycarbohydratefatty acidsgenotoxicityBromelia antiacantha (known as wild banana) is traditionally used to treat coughs, as an emollient and antitussive, and to treat mouth and skin ulcers. To investigate the radical scavenger properties, DPPH and phosphomolybdenium were used, with low antioxidant activity being observed for both (below 5 mg/ml of extract presented no activity for both techniques). An HPLC fingerprint of phenolic compounds was developed and total phenolics through Folin-Ciocalteau method was an average of 500 µg/g of fresh fruits. Any cytotoxicity was observed on L929 fibroblasts cells for aqueous, methanol and lipid extracts between 500 and 0.01 µg/ ml. The wild banana extract (1000 µg/mL) shown a lower genotoxicity when compared to the negative and positive (MMS) controls. Abundant calcium oxalate crystals and sucrose were found and characterized through NMR and X-ray diffraction respectively. Its centesimal composition indicated abundant carbohydrates (45%) and lipids (18%). The carbohydrate analysis showed acidic monosaccharides, and the lipid analysis shown palmitic and linoleic acids in similar quantities (30% each) and oleic acid 20%, among others, through GC-FID. The viscosity of the water-soluble polysaccharide sub-fraction (5 g/L) was similar to that of water.Sociedade Brasileira de Farmacognosia2009-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000300004Revista Brasileira de Farmacognosia v.19 n.2a 2009reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2009000300004info:eu-repo/semantics/openAccessSantos,Vanessa N. C.Freitas,Rilton A. deDeschamps,Francisco C.Biavatti,Maique W.eng2009-09-01T00:00:00Zoai:scielo:S0102-695X2009000300004Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2009-09-01T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profile
title Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profile
spellingShingle Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profile
Santos,Vanessa N. C.
Bromelia antiacantha
antioxidant
cytotoxicity
carbohydrate
fatty acids
genotoxicity
title_short Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profile
title_full Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profile
title_fullStr Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profile
title_full_unstemmed Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profile
title_sort Ripe fruits of Bromelia antiacantha: investigations on the chemical and bioactivity profile
author Santos,Vanessa N. C.
author_facet Santos,Vanessa N. C.
Freitas,Rilton A. de
Deschamps,Francisco C.
Biavatti,Maique W.
author_role author
author2 Freitas,Rilton A. de
Deschamps,Francisco C.
Biavatti,Maique W.
author2_role author
author
author
dc.contributor.author.fl_str_mv Santos,Vanessa N. C.
Freitas,Rilton A. de
Deschamps,Francisco C.
Biavatti,Maique W.
dc.subject.por.fl_str_mv Bromelia antiacantha
antioxidant
cytotoxicity
carbohydrate
fatty acids
genotoxicity
topic Bromelia antiacantha
antioxidant
cytotoxicity
carbohydrate
fatty acids
genotoxicity
description Bromelia antiacantha (known as wild banana) is traditionally used to treat coughs, as an emollient and antitussive, and to treat mouth and skin ulcers. To investigate the radical scavenger properties, DPPH and phosphomolybdenium were used, with low antioxidant activity being observed for both (below 5 mg/ml of extract presented no activity for both techniques). An HPLC fingerprint of phenolic compounds was developed and total phenolics through Folin-Ciocalteau method was an average of 500 µg/g of fresh fruits. Any cytotoxicity was observed on L929 fibroblasts cells for aqueous, methanol and lipid extracts between 500 and 0.01 µg/ ml. The wild banana extract (1000 µg/mL) shown a lower genotoxicity when compared to the negative and positive (MMS) controls. Abundant calcium oxalate crystals and sucrose were found and characterized through NMR and X-ray diffraction respectively. Its centesimal composition indicated abundant carbohydrates (45%) and lipids (18%). The carbohydrate analysis showed acidic monosaccharides, and the lipid analysis shown palmitic and linoleic acids in similar quantities (30% each) and oleic acid 20%, among others, through GC-FID. The viscosity of the water-soluble polysaccharide sub-fraction (5 g/L) was similar to that of water.
publishDate 2009
dc.date.none.fl_str_mv 2009-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000300004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-695X2009000300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.19 n.2a 2009
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
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reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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