Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ornamental Horticulture (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000400341 |
Resumo: | ABSTRACT An alternative for the conservation of orchid species threatened with extinction is the seeds preservation for long periods by cryopreservation. This work aimed to evaluate the influence of cryogenic solutions on the orchids Epidendrum radicans and Arundina bambusifolia seeds cryopreservation in liquid nitrogen. Seven treatments were performed: 0.4 M sucrose; 2 M glycerol; PVS1; PVS2; PVS3; PVS2 + 1% phloroglucinol and the control. After liquid nitrogen removal, half of the seeds were subjected to tetrazolium test, and another half was in vitro cultured. After 30 days seed germination was evaluated. There was no significant difference between A. bambusifolia and E. radicans in tetrazolium test and seeds germination. On the other hand, it was observed that in A. bambusifolia tetrazolium and seed germination tests the solutions of PVS1, PVS2, PVS2 + 1% phloroglucinol showed the best results. E. radicans seeds with 4% water content cryopreservation does not require the use of cryoprotectant. On the other hand, cryoprotectant's use resulted in positive effect in maintenance and viability preservation of A. bambusifolia seeds. PVS1, PVS2 and PVS2 + 1% phloroglucinol showed best seeds survival rates after cryopreservation in liquid nitrogen. |
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Ornamental Horticulture (Campinas) |
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Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogenEpidendrum radicansArundina bambusifoliaOrchidaceaetetrazoliumseed bankABSTRACT An alternative for the conservation of orchid species threatened with extinction is the seeds preservation for long periods by cryopreservation. This work aimed to evaluate the influence of cryogenic solutions on the orchids Epidendrum radicans and Arundina bambusifolia seeds cryopreservation in liquid nitrogen. Seven treatments were performed: 0.4 M sucrose; 2 M glycerol; PVS1; PVS2; PVS3; PVS2 + 1% phloroglucinol and the control. After liquid nitrogen removal, half of the seeds were subjected to tetrazolium test, and another half was in vitro cultured. After 30 days seed germination was evaluated. There was no significant difference between A. bambusifolia and E. radicans in tetrazolium test and seeds germination. On the other hand, it was observed that in A. bambusifolia tetrazolium and seed germination tests the solutions of PVS1, PVS2, PVS2 + 1% phloroglucinol showed the best results. E. radicans seeds with 4% water content cryopreservation does not require the use of cryoprotectant. On the other hand, cryoprotectant's use resulted in positive effect in maintenance and viability preservation of A. bambusifolia seeds. PVS1, PVS2 and PVS2 + 1% phloroglucinol showed best seeds survival rates after cryopreservation in liquid nitrogen.Sociedade Brasileira de Floricultura e Plantas Ornamentais2018-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000400341Ornamental Horticulture v.24 n.4 2018reponame:Ornamental Horticulture (Campinas)instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)instacron:SBFPO10.14295/oh.v24i4.1180info:eu-repo/semantics/openAccessPaula,Jean Carlo Baudraz deBertoncelli,Douglas JuniorAlves,Guilherme Augusto CitoMen,Gabriel BarracaMathias,Tatiana FattoriFaria,Ricardo Tadeu deeng2019-02-20T00:00:00Zoai:scielo:S2447-536X2018000400341Revistahttp://ornamentalhorticulture.emnuvens.com.br/rbho/indexhttps://old.scielo.br/oai/scielo-oai.php||revista.ornamentalhorticulture@gmail.com2447-536X2447-536Xopendoar:2019-02-20T00:00Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)false |
dc.title.none.fl_str_mv |
Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen |
title |
Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen |
spellingShingle |
Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen Paula,Jean Carlo Baudraz de Epidendrum radicans Arundina bambusifolia Orchidaceae tetrazolium seed bank |
title_short |
Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen |
title_full |
Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen |
title_fullStr |
Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen |
title_full_unstemmed |
Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen |
title_sort |
Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen |
author |
Paula,Jean Carlo Baudraz de |
author_facet |
Paula,Jean Carlo Baudraz de Bertoncelli,Douglas Junior Alves,Guilherme Augusto Cito Men,Gabriel Barraca Mathias,Tatiana Fattori Faria,Ricardo Tadeu de |
author_role |
author |
author2 |
Bertoncelli,Douglas Junior Alves,Guilherme Augusto Cito Men,Gabriel Barraca Mathias,Tatiana Fattori Faria,Ricardo Tadeu de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Paula,Jean Carlo Baudraz de Bertoncelli,Douglas Junior Alves,Guilherme Augusto Cito Men,Gabriel Barraca Mathias,Tatiana Fattori Faria,Ricardo Tadeu de |
dc.subject.por.fl_str_mv |
Epidendrum radicans Arundina bambusifolia Orchidaceae tetrazolium seed bank |
topic |
Epidendrum radicans Arundina bambusifolia Orchidaceae tetrazolium seed bank |
description |
ABSTRACT An alternative for the conservation of orchid species threatened with extinction is the seeds preservation for long periods by cryopreservation. This work aimed to evaluate the influence of cryogenic solutions on the orchids Epidendrum radicans and Arundina bambusifolia seeds cryopreservation in liquid nitrogen. Seven treatments were performed: 0.4 M sucrose; 2 M glycerol; PVS1; PVS2; PVS3; PVS2 + 1% phloroglucinol and the control. After liquid nitrogen removal, half of the seeds were subjected to tetrazolium test, and another half was in vitro cultured. After 30 days seed germination was evaluated. There was no significant difference between A. bambusifolia and E. radicans in tetrazolium test and seeds germination. On the other hand, it was observed that in A. bambusifolia tetrazolium and seed germination tests the solutions of PVS1, PVS2, PVS2 + 1% phloroglucinol showed the best results. E. radicans seeds with 4% water content cryopreservation does not require the use of cryoprotectant. On the other hand, cryoprotectant's use resulted in positive effect in maintenance and viability preservation of A. bambusifolia seeds. PVS1, PVS2 and PVS2 + 1% phloroglucinol showed best seeds survival rates after cryopreservation in liquid nitrogen. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000400341 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000400341 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.14295/oh.v24i4.1180 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Floricultura e Plantas Ornamentais |
publisher.none.fl_str_mv |
Sociedade Brasileira de Floricultura e Plantas Ornamentais |
dc.source.none.fl_str_mv |
Ornamental Horticulture v.24 n.4 2018 reponame:Ornamental Horticulture (Campinas) instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) instacron:SBFPO |
instname_str |
Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) |
instacron_str |
SBFPO |
institution |
SBFPO |
reponame_str |
Ornamental Horticulture (Campinas) |
collection |
Ornamental Horticulture (Campinas) |
repository.name.fl_str_mv |
Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) |
repository.mail.fl_str_mv |
||revista.ornamentalhorticulture@gmail.com |
_version_ |
1754734900242546688 |