The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ornamental Horticulture (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2019000400450 |
Resumo: | Abstract T. kaufmanniana is a threatened and endemic species of the Tien Shan Mountains with a complex of valuable ornamental features. However, the commercial usage of the plant is limited due to the restricted availability of the bulbs in nature, difficulties in overcoming seed dormancy and low efficiency of the species reproduction. Results of the investigation of the reproductive biology of this species in the culture conditions allowed us to characterize T. kaufmanniana as viable with successful seedage. In the work reported here the effect of low temperature on the proper development of embryos is addressed, and an attempt is made to clarify the effect of cytokinins (TDZ, BAP) and auxin (NAA) on the regeneration capacity of isolated T. kaufmanniana embryos for the adventitious shoots and bulblets formation. Direct shoot organogenesis was induced by the combined action of chilling and BAP or TDZ treatment. Among the variants tested, TDZ promoted the formation of adventitious bulbs in 48 % of the chilled embryos, compared to non-chilled embryos (0.0%) and embryos exposed to the same concentration of BAP (39%). The best culture condition for T. kaufmanniana embryos growth and bulblets formation consisted of chilling for 10 wk in the dark at 4 °C, 4 wk at 20 °C under 16 h light photoperiod and 10 wk at 4 °C in the dark. Sufficiently effective protocol for clonal micropropagation was developed for the first time for T. kaufmanniana by the use of immature zygotic embryos as primary explants. |
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Ornamental Horticulture (Campinas) |
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The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditionsTulipa speciesseed productionembryo cultureimmature zygotic embryoschillingbulblet formationgrowth regulatorsAbstract T. kaufmanniana is a threatened and endemic species of the Tien Shan Mountains with a complex of valuable ornamental features. However, the commercial usage of the plant is limited due to the restricted availability of the bulbs in nature, difficulties in overcoming seed dormancy and low efficiency of the species reproduction. Results of the investigation of the reproductive biology of this species in the culture conditions allowed us to characterize T. kaufmanniana as viable with successful seedage. In the work reported here the effect of low temperature on the proper development of embryos is addressed, and an attempt is made to clarify the effect of cytokinins (TDZ, BAP) and auxin (NAA) on the regeneration capacity of isolated T. kaufmanniana embryos for the adventitious shoots and bulblets formation. Direct shoot organogenesis was induced by the combined action of chilling and BAP or TDZ treatment. Among the variants tested, TDZ promoted the formation of adventitious bulbs in 48 % of the chilled embryos, compared to non-chilled embryos (0.0%) and embryos exposed to the same concentration of BAP (39%). The best culture condition for T. kaufmanniana embryos growth and bulblets formation consisted of chilling for 10 wk in the dark at 4 °C, 4 wk at 20 °C under 16 h light photoperiod and 10 wk at 4 °C in the dark. Sufficiently effective protocol for clonal micropropagation was developed for the first time for T. kaufmanniana by the use of immature zygotic embryos as primary explants.Sociedade Brasileira de Floricultura e Plantas Ornamentais2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2019000400450Ornamental Horticulture v.25 n.4 2019reponame:Ornamental Horticulture (Campinas)instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)instacron:SBFPO10.1590/2447-536x.v25i4.2035info:eu-repo/semantics/openAccessYurievna,Nabieva AlexandraVladimirovna,Gerasimovich Lyudmilaeng2020-01-21T00:00:00Zoai:scielo:S2447-536X2019000400450Revistahttp://ornamentalhorticulture.emnuvens.com.br/rbho/indexhttps://old.scielo.br/oai/scielo-oai.php||revista.ornamentalhorticulture@gmail.com2447-536X2447-536Xopendoar:2020-01-21T00:00Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)false |
dc.title.none.fl_str_mv |
The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions |
title |
The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions |
spellingShingle |
The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions Yurievna,Nabieva Alexandra Tulipa species seed production embryo culture immature zygotic embryos chilling bulblet formation growth regulators |
title_short |
The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions |
title_full |
The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions |
title_fullStr |
The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions |
title_full_unstemmed |
The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions |
title_sort |
The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions |
author |
Yurievna,Nabieva Alexandra |
author_facet |
Yurievna,Nabieva Alexandra Vladimirovna,Gerasimovich Lyudmila |
author_role |
author |
author2 |
Vladimirovna,Gerasimovich Lyudmila |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Yurievna,Nabieva Alexandra Vladimirovna,Gerasimovich Lyudmila |
dc.subject.por.fl_str_mv |
Tulipa species seed production embryo culture immature zygotic embryos chilling bulblet formation growth regulators |
topic |
Tulipa species seed production embryo culture immature zygotic embryos chilling bulblet formation growth regulators |
description |
Abstract T. kaufmanniana is a threatened and endemic species of the Tien Shan Mountains with a complex of valuable ornamental features. However, the commercial usage of the plant is limited due to the restricted availability of the bulbs in nature, difficulties in overcoming seed dormancy and low efficiency of the species reproduction. Results of the investigation of the reproductive biology of this species in the culture conditions allowed us to characterize T. kaufmanniana as viable with successful seedage. In the work reported here the effect of low temperature on the proper development of embryos is addressed, and an attempt is made to clarify the effect of cytokinins (TDZ, BAP) and auxin (NAA) on the regeneration capacity of isolated T. kaufmanniana embryos for the adventitious shoots and bulblets formation. Direct shoot organogenesis was induced by the combined action of chilling and BAP or TDZ treatment. Among the variants tested, TDZ promoted the formation of adventitious bulbs in 48 % of the chilled embryos, compared to non-chilled embryos (0.0%) and embryos exposed to the same concentration of BAP (39%). The best culture condition for T. kaufmanniana embryos growth and bulblets formation consisted of chilling for 10 wk in the dark at 4 °C, 4 wk at 20 °C under 16 h light photoperiod and 10 wk at 4 °C in the dark. Sufficiently effective protocol for clonal micropropagation was developed for the first time for T. kaufmanniana by the use of immature zygotic embryos as primary explants. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2019000400450 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2019000400450 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2447-536x.v25i4.2035 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Floricultura e Plantas Ornamentais |
publisher.none.fl_str_mv |
Sociedade Brasileira de Floricultura e Plantas Ornamentais |
dc.source.none.fl_str_mv |
Ornamental Horticulture v.25 n.4 2019 reponame:Ornamental Horticulture (Campinas) instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) instacron:SBFPO |
instname_str |
Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) |
instacron_str |
SBFPO |
institution |
SBFPO |
reponame_str |
Ornamental Horticulture (Campinas) |
collection |
Ornamental Horticulture (Campinas) |
repository.name.fl_str_mv |
Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) |
repository.mail.fl_str_mv |
||revista.ornamentalhorticulture@gmail.com |
_version_ |
1754734900617936896 |